Isolation, identification and characterization of cadmium-resistant Pseudomonas aeruginosa strain E1

  • Xiao-xi Zeng (曾晓希)
  • Jian-xin Tang (汤建新)
  • Xue-duan Liu (刘学端)Email author
  • Pei Jiang (蒋 佩)


Strain E1 with resistance to 18 mmol/L cadmium (Cd), isolated from Cd-contaminated soil was identified by morphological observation, biochemical and physiological characterization and 16S rDNA sequence analysis. The resistance to heavy metals Cd, Cu, Co, Mn, Pb, Zn and 12 antibiotics was examined. The ability of removing Cd from solution was studied. The characterizations show that strain E1 is affiliated to Pseudomonas aeruginosa (P. aeruginosa). Strain E1 has high resistance to heavy metals and the order is found to be Cd>Mn>Zn>Cu>Pb>Co in solid media. Strain E1 also exhibits the resistance to 12 antibiotics. Both living and non-living cells of strain E1 can remove Cd from solution, and living cell has better biosorption than non-living cell.

Key words

identification P. aeruginosa cadmium-resistance screening 


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  1. [1]
    DENG X, YI X E, LIU G. Cadmium removal from aqueous solution by gene-modified Escherichia coli JM109 [J]. Journal of Hazardous Materials, 2007, 139(2): 340–344.CrossRefGoogle Scholar
  2. [2]
    ABOU-SHANAB R A I, BERKUM P, ANGLE J S. Heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in Ni-rich serpentine soil and in the rhizosphere of Alyssum murale [J]. Chemosphere, 2007, 68(2): 360–367.CrossRefGoogle Scholar
  3. [3]
    BASTA N T, GRADWOHL R, SNETHEN K L, SCHRODER J L. Chemical immobilization of lead, zinc, and cadmium in smelter-contaminated soils using biosolids and rock phosphate [J]. Journal Environment Quality, 2001, 30(4): 1222–1230.CrossRefGoogle Scholar
  4. [4]
    FILIPIC M, FATUR T, VUDRAG M. Molecular mechanisms of cadmium induced mutagenicity [J]. Human and Experimental Toxicology, 2006, 25(2): 67–77.CrossRefGoogle Scholar
  5. [5]
    GARY G, SCHWARTZ, ISILDINHA M. Is cadmium a cause of human pancreatic cancer? [J]. Cancer Epidemiol Biomarkers Prev, 2000, 9(2): 139–145.Google Scholar
  6. [6]
    BABUK R, RAJMOHAN H R R, RAJANB K M, KARUNA K M. Plasma lipid peroxidation and erythrocyte antioxidant enzymes status in workers exposed to cadmium [J]. Toxicology and Industrial Health, 2006, 22(8): 329–335.CrossRefGoogle Scholar
  7. [7]
    YANG Z Q, YANG S F, QIAN S Y, HONG J S, KADIISKA M B, TENNANT R W, WAALKES M P, LIU J. Cadmium-induced toxicity in rat primary mid-brain neuroglia cultures: Role of oxidative stress from microglia [J]. Toxicol Sci, 2007, 98(2): 488–494.CrossRefGoogle Scholar
  8. [8]
    BRZÓSKA M M, MONIUSZKO-JAKONIUK J. Low-level exposure to cadmium during the lifetime increases the risk of osteoporosis and fractures of the lumbar spine in the elderly: Studies on a rat model of human environmental exposure [J]. Toxicol Sci, 2004, 82(2): 468–477.CrossRefGoogle Scholar
  9. [9]
    ANA N A, ELIZABETH S, SHARRETT A R, CALDERON E. Lead, cadmium, smoking, and increased risk of peripheral arterial disease [J]. Circulation, 2004, 109(6): 3196–3201.Google Scholar
  10. [10]
    SHIN H J, LEE B H, YEO M G, OH S H, PARK J D, PARK K K. Induction of orphan nuclear receptor Nur77 gene expression and its role in cadmium-induced apoptosis in lung [J]. Carcinogenesis, 2004, 25(8): 1467–1475.CrossRefGoogle Scholar
  11. [11]
    GRASSESCHI R M, RAMASWAMY R B, LEVINE D J, KLAASSEN C D, WESSELIUS L J. Cadmium accumulation and detoxification by alveolar macrophages of cigarette smokers [J]. Chest, 2003, 124(5): 1924–1928.CrossRefGoogle Scholar
  12. [12]
    NISHIJO M, MORIKAWA Y, NAKAGAWA H, TAWARA K, MIURA K, KIDO T, IKAWA A, KOBAYASHI E, NOGAWA K. Causes of death and renal tubular dysfunction in residents exposed to cadmium in the environment [J]. Occup Environ Med, 2006, 63(8): 545–550.CrossRefGoogle Scholar
  13. [13]
    ZIAGOVA M, DIMITRIADIS G, ASLANIDOU D, PAPAIOANNOU X, LITOPOULOU T E, LIAKOPOULOU K M. Comparative study of Cd(II) and Cr(VI) biosorption on Staphylococcus xylosus and Pseudomonas sp. in single and binary mixtures[J]. Biosensors Technol, 2007, 98(15): 2859–2865.CrossRefGoogle Scholar
  14. [14]
    QIN Wen-qing, LAN Zhuo-yue, LI Wei-zhong. Recovery of zinc from low-grade zinc oxide ores by solvent extraction [J]. Journal of Central South University of Technology, 2003, 10(2): 98–102.CrossRefGoogle Scholar
  15. [15]
    YU Jun-xia, TONG Mi, SUN Xiao-mei, LI Bu-hai. Biomass grafted with polyamic acid for enhancement of cadmium (II) and lead (II) biosorption [J]. Biochemical Engineering Journal, 2007, 33(2): 126–133.CrossRefGoogle Scholar
  16. [16]
    HINOJOSA M B, CARREIRA J A, ROBERTO G R, RICHARD P D. Microbial response to heavy metal-polluted soils: Community analysis from phospholipid-linked fatty acids and ester-linked fatty acids extracts [J]. J Environ Qual, 2005, 34(5): 1789–1800.CrossRefGoogle Scholar
  17. [17]
    ZOUBOULIS A I, LOUKIDOU M X, MATIS K A. Biosorption of toxic metals from aqueous solutions by bacteria strains isolated from metal-polluted soils [J]. Process Biochemistry, 2004, 39(8): 909–916.CrossRefGoogle Scholar
  18. [18]
    OZDEMIR G, CEYHAN N, OZTURK T, AKIRMAK F, COSAR T. Biosorption of chromium (VI), cadmium (II) and copper (II) by Pantoea sp. TEM18 [J]. Chemical Engineering Journal, 2004, 102(3): 249–253.CrossRefGoogle Scholar
  19. [19]
    LEBEAU T, BAGOT D, JÉZÉQUEL B, FABRE B. Cadmium biosorption by free and immobilised microorganisms cultivated in a liquid soil extract medium: Effects of Cd, pH and techniques of culture [J]. The Science of the Total Environment, 2002, 291(1/3): 73–83.CrossRefGoogle Scholar
  20. [20]
    YU Xia, CHAI Li-yuan, MIN Xiao-bo. Removal of lead in wastewater by immobilized inactivated cells of rhizopus oligosporus [J]. Journal of Central South University of Technology, 2003, 10(4): 313–317.CrossRefGoogle Scholar
  21. [21]
    DONG Xiu-zhu, CAI Miao-ying. Manual of common determinative bacteriology [M]. Beijing: Science Press, 2001. (in Chinese)Google Scholar
  22. [22]
    ZHOU Hong-bo, ZENG Xiao-xi, IU Fei-fei, HU Yue-hua. Screening, identification and desilication of a silicate bacteria [J]. Journal of Central South University of Technology, 2006, 13(4): 337–341.CrossRefGoogle Scholar
  23. [23]
    YILMAZ E I. Metal tolerance and biosorption capacity of Bacillus circulars strain EB1 [J]. Research in Microbiology, 2003, 154(6): 409–415.CrossRefGoogle Scholar
  24. [24]
    VERMA T, SRINATH T, GADPAYLE R U, RAMTEKE P W, HANS R K, GARG S K. Chromate tolerant bacteria isolated from tannery effluent [J]. Bioresource Technol, 2001, 78(1): 31–35.CrossRefGoogle Scholar
  25. [25]
    HU Qing, DOU Min-na, QI Hong-yan, XIE Xiang-ming, ZHUANG Guo-qiang, YANG Min. Detection isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE [J]. Journal of Environmental Sciences, 2007, 19(9): 1114–1119.CrossRefGoogle Scholar
  26. [26]
    GUPTA G, KEEGAN B. Bioaccumulation and biosorption of lead by poultry litter microorganisms [J]. Poultry Science, 1998, 77(3): 400–404.CrossRefGoogle Scholar
  27. [27]
    KEFALA M I, ZOUBOULIS A I, MATIS K A. Biosorption of cadmium ions by actinomycetes and separation by flotation [J]. Environmental Pollution, 1999, 104(2): 283–293.CrossRefGoogle Scholar
  28. [28]
    TANGAROMSUK J, POKETHITIYOOK P, KRUATRACHUE M, UPATHAM E S. Cadmium biosorption by sphingomonas paucimobilis biomass [J]. Bioresource Technology, 2002, 85(1): 103–105.CrossRefGoogle Scholar

Copyright information

© Central South University Press and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Xiao-xi Zeng (曾晓希)
    • 1
    • 2
  • Jian-xin Tang (汤建新)
    • 2
  • Xue-duan Liu (刘学端)
    • 1
    Email author
  • Pei Jiang (蒋 佩)
    • 2
  1. 1.School of Resources Processing and BioengineeringCentral South UniversityChangshaChina
  2. 2.College of Packaging and PrintingHunan University of TechnologyZhuzhouChina

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