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Basic fibroblast growth factor attenuates left-ventricular remodeling following surgical ventricular restoration in a rat ischemic cardiomyopathy model

  • Atsushi Nagasawa
  • Hidetoshi Masumoto
  • Shigeki Yanagi
  • Naoki Kanemitsu
  • Tadashi Ikeda
  • Yasuhiko Tabata
  • Kenji MinatoyaEmail author
Original Article
  • 29 Downloads

Abstract

Objective

Although surgical ventricular restoration for ischemic cardiomyopathy is expected as an alternative or bridge to heart transplantation, post-operative remodeling of left ventricle (LV) needs to be addressed. This study aimed to examine the effect of basic fibroblast growth factor (bFGF), which induces angiogenesis and tissue regeneration in ischemic myocardium, to prevent remodeling after surgical ventricular restoration (SVR) using a rat ischemic cardiomyopathy model.

Methods

Four weeks after coronary artery ligation, rats were divided into two groups: rats treated with SVR alone (SVR; n = 21), and rats treated with SVR and local sustained release of bFGF using gelatin hydrogel sheet (SVR + bFGF; n = 22). Cardiac function was assessed by serial echocardiography and cardiac catheterization. Cardiac tissue sections were histologically examined for vascular density and fibrosis.

Results

Higher systolic function and lower LV end-diastolic pressure (LVEDP) were observed in rats treated with SVR + bFGF (SVR vs SVR + bFGF; Ees: 0.22 ± 0.11 vs 0.33 ± 0.22 mmHg/μL, p = 0.0328; LVEDP: 12.7 ± 7.0 vs 8.5 ± 4.3 mmHg, p = 0.0230). LV area tended to be lower in rats treated with SVR + bFGF compared to rats treated with SVR alone (left-ventricular end-diastolic area: 0.66 ± 0.07 vs 0.62 ± 0.07 cm2, p = 0.071). Vascular density tended to be higher in rats treated with SVR + bFGF than those without bFGF (23.3 ± 8.1 vs 28.8 ± 9.5/mm2, p = 0.0509).

Conclusions

BFGF induced angiogenesis and attenuated remodeling after SVR which secured the efficacy of SVR in a rat ischemic cardiomyopathy model.

Keywords

Basic fibroblast growth factor Surgical ventricular restoration Ischemic cardiomyopathy Left-ventricular remodeling 

Notes

Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan (to T.I.) [Grant Number 22591540]. We are grateful to Mrs. F. Kataoka (Kyoto Univ.) for assistances in histological studies, and to Dr. A. Sugimoto (Kyoto Univ.) for assistance with surgical procedures and examinations such as echocardiography and cardiac catheterization. We thank Dr. Hemant Poudyal (Kyoto Univ.) for critical reading of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors have declared that no conflict of interest exists.

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Copyright information

© The Japanese Association for Thoracic Surgery 2019

Authors and Affiliations

  1. 1.Department of Cardiovascular Surgery, Graduate School of MedicineKyoto UniversityKyotoJapan
  2. 2.Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical SciencesKyoto UniversityKyotoJapan
  3. 3.Department of Cardiovascular SurgeryKokura Kinen HospitalKitakyushuJapan
  4. 4.Department of Cardiovascular SurgeryKumamoto Central HospitalKumamotoJapan
  5. 5.Department of Cardiovascular SurgeryJapanese Red Cross Wakayama Medical CenterWakayamaJapan

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