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Lipids

, Volume 52, Issue 9, pp 781–787 | Cite as

Anti-Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF-κB and MAP-Kinase Pathways in Macrophages

  • Jeehye Sung
  • Heemang Jeon
  • In-Hwan Kim
  • Heon Sang Jeong
  • Junsoo LeeEmail author
Communication

Abstract

Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.

Keywords

Stearidonic acid Echium oil Anti-inflammatory NFκB (nuclear factor κB) MAPK (mitogen-activated protein kinases) pathway 

Abbreviations

ALA

α-Linolenic acid

BSA

Bovine serum albumin

DHA

Docosahexaenoic acid

DMEM

Dulbecco’s modified Eagle’s medium

FBS

Foetal bovine serum

EPA

Eicosapentaenoic acid

ERK

Extracellular signal regulated kinase

JNK

c-jun N terminal kinase

iNOS

Inducible nitric oxide synthase

IκB

Inhibitor of κB

LDH

Lactate dehydrogenase

LPS

Lipopolysaccharide

MAPK

Mitogen-activated protein kinases

MTT

3-(4,5-Dimethyl thiazol 2-yl)-2,5-diphenyltetrazolium

NO

Nitric oxide

NFκB

Nuclear factor κB

n-3 PUFA

Omega-3 polyunsaturated fatty acids

PBS

Dulbecco’s phosphate-buffered saline

SDA

Stearidonic acid

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Copyright information

© AOCS 2017

Authors and Affiliations

  • Jeehye Sung
    • 1
  • Heemang Jeon
    • 2
  • In-Hwan Kim
    • 3
  • Heon Sang Jeong
    • 1
  • Junsoo Lee
    • 1
    Email author
  1. 1.Division of Food and Animal Sciences, College of Agriculture, Life, and Environmental SciencesChungbuk National UniversityCheongjuKorea
  2. 2.Research and Innovation CenterCosmax Bio Inc.SeongnamKorea
  3. 3.Department of Food and NutritionKorea UniversitySeoulKorea

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