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Urine and serum NMR-based metabolomics in pre-procedural prediction of contrast-induced nephropathy

  • Nooshin Dalili
  • Saeed Chashmniam
  • Seyed Mojtaba Heydari Khoormizi
  • Lida Salehi
  • Seyed Ali Jamalian
  • Mohsen Nafar
  • Shiva KalantariEmail author
IM - ORIGINAL

Abstract

Contrast induced nephropathy (CIN) has been reported to be the third foremost cause of acute renal failure. Metabolomics is a robust technique that has been used to identify potential biomarkers for the prediction of renal damage. We aim to analyze the serum and urine metabolites changes, before and after using contrast for coronary angiography, to determine if metabolomics can predict early development of CIN. 66 patients undergoing elective coronary angiography were eligible for enrollment. Urine and serum samples were collected prior to administration of CM and 72 h post procedure and analyzed by nuclear magnetic resonance. The significant differential metabolites between patients who develop CIN and patients who have stable renal function after angiography were identified using U test and receiver operating characteristic analysis was performed for each metabolite candidate. Potential susceptible pathways to cytotoxic effect of CM were investigated by pathway analysis. A predictive panel composed of six urinary metabolites had the best area under the curve. Glutamic acid, uridine diphosphate, glutamine and tyrosine were the most important serum predictive biomarkers. Several pathways related to amino acid and nicotinamide metabolism were suggested as impaired pathways in CIN prone patients. Changes exist in urine and serum metabolomics patterns in patients who do and do not develop CIN after coronary angiography hence metabolites may be potential predictive identifiers of CIN.

Keywords

Contrast induced nephropathy Metabolomics Nuclear magnetic resonance Biomarker 

Notes

Acknowledgements

The authors like to thank the Chronic Kidney Disease Research Center (CKDRC) at Shahid Beheshti University of Medical Sciences for its financial support.

Funding

This work was supported by the Chronic Kidney Disease Research Center (CKDRC) and Urology and Nephrology Research Center (UNRC) at Shahid Beheshti University of Medical Sciences [462/27].

Compliance with ethical standards

Conflict of interest

There is nothing to disclose in relation to this manuscript.

Statement of human and animal rights

This study was approved by the ethical committee of the Shahid Beheshti University of Medical Sciences.

Informed consent

All patients signed an informed consent.

Supplementary material

11739_2019_2128_MOESM1_ESM.xlsx (185 kb)
Supplementary file1 (XLSX 185 kb)
11739_2019_2128_MOESM2_ESM.xlsx (194 kb)
Supplementary file2 (XLSX 193 kb)

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Copyright information

© Società Italiana di Medicina Interna (SIMI) 2019

Authors and Affiliations

  • Nooshin Dalili
    • 1
  • Saeed Chashmniam
    • 2
  • Seyed Mojtaba Heydari Khoormizi
    • 3
  • Lida Salehi
    • 3
  • Seyed Ali Jamalian
    • 4
  • Mohsen Nafar
    • 3
  • Shiva Kalantari
    • 3
    Email author
  1. 1.Urology and Nephrology Research CenterShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Department of ChemistrySharif University of TechnologyTehranIran
  3. 3.Chronic Kidney Disease Research CenterShahid Labbafinejad Medical Center, Shahid Beheshti University of Medical SciencesTehranIran
  4. 4.Department of CardiologyShahid Lavasani Medical CenterTehranIran

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