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Acta Physiologiae Plantarum

, 42:21 | Cite as

Different proline responses of two Algerian durum wheat cultivars to in vitro salt stress

  • Katia Ami
  • Séverine Planchais
  • Cécile Cabassa
  • Anne Guivarc’h
  • Anne-Aliénor Very
  • Majda Khelifi
  • Réda Djebbar
  • Ouzna Abrous-Belbachir
  • Pierre CarolEmail author
Original Article
  • 57 Downloads

Abstract

Durum wheat, Triticum turgidum subsp. durum (Desf.) Husn., is one of the most salt-sensitive cereal crops, but the physiological responses of different cultivars to salt stress vary. Cultivars that are suited to arid conditions like in Algeria may not necessarily be tolerant to increased salinity. When 10-day seedlings of Algerian durum wheat varieties Hedba 3 (HD3) and Mohamed Ben Bachir (MBB) were subjected to salt stress, they accumulated proline and expressed stress-related and proline metabolism genes in a classic salt-stress response. Expression of the selective sodium transporter genes HKT1;4-1 and -2 was found to be organ-specific and modulated by salt stress in both cultivars. Adding proline to the salt-containing growth medium alleviated some salt stress effects such as decrease in water content, ion leakage and expression oxidative stress markers while growth parameters were partially rescued to different extents in the two cultivars. Durum wheat seedlings accumulated sodium ions (Na+) at the expense of potassium ions (K+) under salt stress which lowered the in planta K+/Na+ ratio. The two durum wheat cultivars studied here respond differently to salt stress in terms of responsiveness to proline, HKT1;4 gene expression, and Na+ and K+ accumulation. Notably, salt stress can be partially alleviated by proline in the drought-resistant cultivar MBB, even though it is relatively salt sensitive.

Keywords

Durum wheat Triticum turgidum Salt stress Proline HKT In vitro 

Notes

Acknowledgements

KA was the recipient of a grant from the Algerian Ministry of Higher Education for PhD training (PNE program). OAB, RD, SP, CC, PC and KA were funded by the French–Algerian exchange program “Partenariat Hubert Curien” Tassili 12MDU854.

Supplementary material

11738_2019_3004_MOESM1_ESM.pdf (253 kb)
Supplementary file1 (PDF 253 kb)
11738_2019_3004_MOESM2_ESM.doc (54 kb)
Supplementary file2 (DOC 54 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2020

Authors and Affiliations

  • Katia Ami
    • 1
    • 2
    • 3
  • Séverine Planchais
    • 1
  • Cécile Cabassa
    • 1
  • Anne Guivarc’h
    • 1
  • Anne-Aliénor Very
    • 4
  • Majda Khelifi
    • 2
  • Réda Djebbar
    • 3
  • Ouzna Abrous-Belbachir
    • 3
  • Pierre Carol
    • 1
    Email author
  1. 1.Institute for Ecology and Environmental Sciences of Paris iEES, UMR 7618 Sorbonne Université, CNRS, INRA, IRD, UPECSorbonne Université, University Paris DiderotParisFrance
  2. 2.ENSAAlgiersAlgeria
  3. 3.Laboratory of Biology and Physiology of OrganismsUniversity of Sciences and Technology Houari BoumedieneAlgiersAlgeria
  4. 4.BPMP, Univ Montpellier, CNRS, INRA, SupAgroMontpellierFrance

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