Advertisement

Comparison of catalase activity in different organs of the potato (Solanum tuberosum L.) cultivars grown under field condition and purification by three-phase partitioning

  • Gurpreet Kaur
  • Shweta Sharma
  • Niranjan DasEmail author
Original Article

Abstract

Catalase (CAT) (H2O2: H2O2 oxidoreductase; EC 1.11.1.6) is capable of directly dismutating moderately reactive H2O2 into H2O and O2, and is regarded as one of the major enzymatic antioxidants in plants. The CAT isozymes are known to be differentially expressed and regulated. In this study, micropropagated potato plantlets of seven cultivars were grown under field condition with uniform agricultural practices. CAT activities were measured in the crude extracts from different potato organs namely tubers, leaves and stems at different stages of their growth. Relatively higher CAT activity was noticed in the very small actively growing tubers as compared to the other tissues. Cultivar-dependent differences were noticed in terms of the CAT activities which clearly indicated variation with regard to their antioxidative capacities. pH profile, thermostability and storage stability of CAT were examined. A simple and rapid three-phase partitioning (TPP) method worked effectively with regard to purification of CAT from the crude extracts. Both denaturing and non-denaturing PAGE analyses suggested that both the tuber-type and leaf-type CAT are tetrameric in nature and varied in size, possibly referred to the distinct isoforms in potato.

Keywords

Potato (Solanum tuberosum L.) cultivars Field condition Catalase (CAT) Three-phase partitioning (TPP) PAGE analyses 

Notes

Acknowledgements

We gracefully thank UGC, Government of India, for providing UGC:MAN Fellowship to G. Kaur.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Human and animal rights statement

This study did not involve human participants and/or animals.

Supplementary material

11738_2019_3002_MOESM1_ESM.tif (2 mb)
Supplementary file 1 (TIF 2025 kb)

References

  1. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126Google Scholar
  2. Agrawal L, Chakraborty S, Jaiswal DK, Gupta S, Datta A, Chakraborty N (2008) Comparative proteomics of tuber induction, development and maturation reveal the complexity of tuberization process in potato (Solanum tuberosum L.). J Proteome Res 7:3803–3817PubMedGoogle Scholar
  3. Beaumont F, Jouve H-M, Gagnon J, Gaillard J, Pelmont J (1990) Purification and properties of a catalase from potato tubers (Solanum tuberosum). Plant Sci 72:19–26Google Scholar
  4. Bienert GP, Møller ALB, Kristiansen KA, Schulz A, Møller IM, Schjoerring JK, Jahn TP (2007) Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes. J Biol Chem 282:1183–1192PubMedGoogle Scholar
  5. Boguszewska D, Grudkowska M, Zagdańska B (2010) Drought-responsive antioxidant enzymes in potato (Solanum tuberosum L.). Pot Res 53:373–382Google Scholar
  6. Bowler C, Slooten L, Vandenbranden S, Rycke RD, Botterman J, Sybesma C, Montagu MV, Inzé D (1991) Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. EMBO J 10:1723–1732PubMedPubMedCentralGoogle Scholar
  7. Cabiscol E, Tamarit J, Ros J (2000) Oxidative stress in bacteria and protein damage by reactive oxygen species. Int Microbiol 3:3–8PubMedGoogle Scholar
  8. Choudhury FK, Rivero RM, Blumwald E, Mittler R (2017) Reactive oxygen species, abiotic stress and stress combination. Plant J 90:856–867PubMedGoogle Scholar
  9. Das K, Roychoudhury A (2014) Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Front Environ Sci 2:53.  https://doi.org/10.3389/fenvs.2014.00053 CrossRefGoogle Scholar
  10. Demagante AL, Vander Zaag P (1988) The response of potato (Solanum spp.) to photoperiod and light intensity under high temperatures. Pot Res 31:73–83Google Scholar
  11. Dennison C, Lovrien R (1997) Three phase partitioning: concentration and purification of proteins. Protein Expres Purif 11:149–161Google Scholar
  12. Du Y-Y, Wang P-C, Chen J, Song C-P (2008) Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana. J Integr Plant Biol 50:1318–1326PubMedGoogle Scholar
  13. Duman YA, Kaya E (2013) Three-phase partitioning as a rapid and easy method for the purification and recovery of catalase from sweet potato tubers (Solanum tuberosum). Appl Biochem Biotechnol 170:1119–1126PubMedGoogle Scholar
  14. El-Soda M, Malosetti M, Zwaan BJ, Koornneef M, Aarts MGM (2014) Genotype x environment interaction QTL mapping in plants: lessons from Arabidopsis. Trends Plant Sci 19:390–398PubMedGoogle Scholar
  15. Fahnenstich H, Scarpeci TE, Valle EM, Flügge U-I, Maurino VG (2008) Generation of hydrogen peroxide in chloroplasts of Arabidopsis overexpressing glycolate oxidase as an inducible system to study oxidative stress. Plant Physiol 148:719–729PubMedPubMedCentralGoogle Scholar
  16. Foyer CH, Noctor G (2005) Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses. Plant Cell 17:1866–1875PubMedPubMedCentralGoogle Scholar
  17. Gapper C, Dolan L (2006) Control of plant development by reactive oxygen species. Plant Physiol 141:341–345PubMedPubMedCentralGoogle Scholar
  18. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930PubMedPubMedCentralGoogle Scholar
  19. Guan L, Scandalios JG (1996) Molecular evolution of maize catalases and their relationship to other eukaryotic and prokaryotic catalases. J Mol Evol 42:570–579PubMedGoogle Scholar
  20. Hammond-Kosack KE, Jones JDG (1996) Resistance gene-dependent plant defense responses. Plant Cell 8:1773–1791PubMedPubMedCentralGoogle Scholar
  21. Hu L, Yang Y, Jiang L, Liu S (2016) The catalase gene family in cucumber: genome-wide identification and organization. Genet Mol Biol 39:408–415PubMedPubMedCentralGoogle Scholar
  22. Kandukuri SS, Noor A, Ranjini SS, Vijayalakshmi MA (2012) Purification and characterization of catalase from sprouted black gram (Vigna mungo) seeds. J Chromatogr B 889–890:50–54Google Scholar
  23. Kawakami S, Mizuno M, Tsuchida H (2000) Comparison of antioxidant enzyme activities between Solanum tuberosum L. cultivars Danshaku and Kitaakari during low-temperature storage. J Agric Food Chem 48:2117–2121PubMedGoogle Scholar
  24. Kiss É, Szamos J, Tamás B, Borbás R (1998) Interfacial behavior of proteins in three-phase partitioning using salt-containing water/tert-butanol systems. Colloids Surf A Physicochem Eng Aspects 142:295–302Google Scholar
  25. Klotz MG, Loewen PC (2003) The molecular evolution of catalatic hydroperoxidases: evidence for multiple lateral transfer of genes between prokaryota and from bacteria into eukaryota. Mol Biol Evol 20:1098–1112PubMedGoogle Scholar
  26. Kumar V, Luthra SK, Bhardwaj V, Singh BP (2014) Indian potato varieties and their salient features. CPRI Technical Bulletin No. 78 (revised), ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh, IndiaGoogle Scholar
  27. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685PubMedGoogle Scholar
  28. Lawlor DW (2002) Limitation to photosynthesis in water-stressed leaves: stomata vs. metabolism and the role of ATP. Ann Bot 89:871–885PubMedPubMedCentralGoogle Scholar
  29. Lopez-Huertas E, Charlton WL, Johnson B, Graham IA, Baker A (2000) Stress induces peroxisome biogenesis genes. EMBO J 19:6770–6777PubMedPubMedCentralGoogle Scholar
  30. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  31. Mhamdi A, Queval G, Chaouch S, Vanderauwera S, Breusegem FV, Noctor G (2010) Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models. J Expt Bot 61:4197–4220Google Scholar
  32. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410PubMedPubMedCentralGoogle Scholar
  33. Mittler R (2017) ROS are good. Trends Plant Sci 22:11–19PubMedGoogle Scholar
  34. Miyagawa Y, Tamoi M, Shigeoka S (2000) Evaluation of the defense system in chloroplasts to photooxidative stress caused by paraquat using transgenic tobacco plants expressing catalase from Escherichia coli. Plant Cell Physiol 41:311–320PubMedGoogle Scholar
  35. Özer B, Akardere E, Çelem EB, Önal S (2010) Three-phase partitioning as a rapid and efficient method for purification of invertase from tomato. Biochem Eng J 50:110–115Google Scholar
  36. Rahnama H, Ebrahimzadeh H (2005) The effect of NaCl on antioxidant enzyme activities in potato seedlings. Biol Plantarum 49:93–97Google Scholar
  37. Rojas-Beltran JA, Dejaeghere F, Abd Alla Kotb M, Du Jardin P (2000) Expression and activity of antioxidant enzymes during potato tuber dormancy. Pot Res 43:383–393Google Scholar
  38. Romero AP, Alarcón A, Valbuena RI, Galeano CH (2017) Physiological assessment of water stress in potato using spectral information. Front Plant Sci 8:1608PubMedPubMedCentralGoogle Scholar
  39. Sarkar D (2008) The signal transduction pathways controlling in planta tuberization in potato: an emerging synthesis. Plant Cell Rep 27:1–8PubMedGoogle Scholar
  40. Saxena L, Iyer BK, Ananthanarayan L (2007) Three phase partitioning as a novel method for purification of ragi (Eleusine coracana) bifunctional amylase/protease inhibitor. Process Biochem 42:491–495Google Scholar
  41. Scandalios JG, Guan L, Polidoros AN (1997) Catalases in plants: gene structure, properties, regulation, and expression. In: Scandalios JG (ed), Oxidative stress and the molecular biology of antioxidant defenses. Cold Spring Harbor Liboratory Press pp 343–406Google Scholar
  42. Schägger H, Jagow GV (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem 199:223–231PubMedGoogle Scholar
  43. Spychalla JP, Desborough SL (1990) Superoxide dismutase, catalase, and α-tocopherol content of stored potato tubers. Plant Physiol 94:1214–1218PubMedPubMedCentralGoogle Scholar
  44. Su Y, Guo J, Ling H, Chen S, Wang S, Xu L, Allan AC, Que Y (2014) Isolation of a novel peroxisomal catalase gene from sugarcane, which is responsive to biotic and abiotic stresses. PLoS ONE 9:e84426.  https://doi.org/10.1371/journal.pone.0084426 CrossRefPubMedPubMedCentralGoogle Scholar
  45. Tan KH, Lovrien R (1972) Enzymology in aqueous-organic cosolvent binary mixtures. J Biol Chem 247:3278–3285PubMedGoogle Scholar
  46. Willekens H, Langebartels C, Tire C, Montagu MV, Inzé D, Camp WV (1994) Differential expression of catalase genes in Nicotiana plumbaginifolia (L.). Proc Natl Acad Sci USA 91:10450–10454PubMedGoogle Scholar
  47. Willekens H, Chamnongpol S, Davey M, Schraudner M, Langebartels C, Montagu MV, Inzé D, Camp WV (1997) Catalase is a sink for H2O2 and is indispensable for stress defence in C-3 plants. EMBO J 16:4806–4816PubMedPubMedCentralGoogle Scholar
  48. Yan J-K, Wang Y-Y, Qiu W-Y, Ma H, Wang Z-B, Wu J-Y (2017) Three-phase partitioning as an elegant and versatile platform applied to non-chromatographic bioseparation processes. Crit Rev Food Sci Nutri.  https://doi.org/10.1080/10408398.2017.1327418 CrossRefGoogle Scholar
  49. Zamocky M, Furtmüller PG, Obinger C (2008) Evolution of catalases from bacteria to humans. Antioxid Redox Signal 10:1527–1548PubMedPubMedCentralGoogle Scholar
  50. Zimmermann P, Heinlein C, Orendi G, Zentgraf U (2006) Senescence-specific regulation of catalases in Arabidopsis thaliana (L.) Heynh. Plant Cell Environ 29:1049–1060PubMedGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2020

Authors and Affiliations

  1. 1.Department of BiotechnologyThapar Institute of Engineering and TechnologyPatialaIndia

Personalised recommendations