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Acta Physiologiae Plantarum

, 41:189 | Cite as

Physiological and biochemical impacts of silicon against water deficit in sugarcane

  • Breno Kennedy Lima Bezerra
  • Giuseppina Pace Pereira Lima
  • André Rodrigues dos Reis
  • Marcelo de Almeida Silva
  • Mônica Sartori de CamargoEmail author
Original Article
  • 101 Downloads

Abstract

Silicon (Si) has been reported to minimize the impacts of water deficit, even though it is not considered an essential plant element. Sugarcane is highly impacted by water deficit and has a particular and complex mechanism to address this stressful condition. Although sugarcane is an Si-accumulating plant, there are few results on the association between Si and water deficit, and physiological and biochemical responses are unclear for this crop. This study investigated the physiological and antioxidant defense system responses in drought-tolerant (RB86-7515) and drought-sensitive (RB85-5536) sugarcane cultivars grown in soil with and without silicon fertilization and subjected to water deficit for 30 and 60 days during the tillering (first experiment) or grand growth (second experiment) phases. Four replications were evaluated in both experiments. Silicon was used at a rate equivalent to 600 kg ha−1 Si as calcium magnesium silicate (108.4 g kg−1 Si; 274 g kg−1 Ca; 481 g kg−1 Mg), which was applied in soil 11 weeks before sugarcane was transplanted. Silicon fertilization improved physiological responses by increasing the water potential and relative water content in the leaves during the tillering and grand growth phases. Additionally, Si increased proline concentrations and/or superoxide dismutase (SOD) and/or ascorbate peroxidase (APX) levels in drought-tolerant and drought-sensitive cultivars under water deficit. These results suggested that Si could play a role in the detoxification of excessive ROS production by increasing proline levels or APX activities in sugarcane grown under water deficit.

Keywords

Beneficial element Plant nutrition Drought Saccharum spp. Antioxidant enzymes 

Abbreviations

APX

Ascorbate peroxidase

CAT

Catalase

Chl a

Chlorophyll a

Chl b

Chlorophyll b

DW

Dry weight

EL

Electrolyte leakage

FW

Fresh weight

MDA

Malondialdehyde

NBT

Nitro blue tetrazolium

POD

Peroxidase

PVP

Polyvinylpyrrolidone

RWC

Relative water content

SOD

Superoxide dismutase

SPAD

Estimated chlorophyll content

SW

Saturated weight

TCA

Trichloroacetic acid

TVD

Top visible dewlap

WD

Water deficit

WW

Well watered

Ψw

Leaf water potential

Notes

Acknowledgements

The authors would like to thank Sao Paulo State Research Foundation (FAPESP) for financial support (Project 2013/04144-7) to the fifth author, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil) for the fellowships to the first (Grant 131385/2013-5), third (Grant 309380/2017-0), and fourth author (Grant 310416/2015-9).

References

  1. Agarie S, Uchida H, Agata W, Kubota F, Kaufman PT (1998) Effects of silicon on transpiration and leaf conductance in rice plants (Oryza sativa L.). Plant Prod Sci. 1:89–95Google Scholar
  2. Ahmed M, Qadeer U, Aslam MA (2014) Silicon application and drought tolerance mechanism of sorghum. Afr J Agr Res 6:594–607Google Scholar
  3. Ahmed M, Qadeer U, Ahmed ZI, Hassan F (2016) Improvement of wheat (Triticum aestivum) drought tolerance by seed priming with silicon. Arch Agron Soil Sci 62:299–335Google Scholar
  4. Alzahrani Y, Kuşvuran A, Alharby HF, Kuşvuran S, Rady MM (2018) The defensive role of silicon in wheat against stress conditions induced by drought, salinity or cadmium. Ecotoxicol Environ Saf 154:187–196PubMedGoogle Scholar
  5. Anderson DL, Bowen JE (1992) Sugarcane nutrition. Potafós, PiracicabaGoogle Scholar
  6. Ashraf M, Rahmatullah R, Afzal M, Ahmed R, Mujeeb F, Sarwar A, Ali L (2010) Alleviation of detrimental effects of NaCl by silicon nutrition in salt-sensitive and salt-tolerant genotypes of sugarcane (Saccharum officinarum L.). Plant Soil 326:381–391Google Scholar
  7. Azevedo RA, Carvalho RF, Cia MC, Gratão P (2011) Sugarcane under pressure: an overview of biochemical and physiological studies of abiotic stress. Trop Plant Biol 4:42–51Google Scholar
  8. Bajji M, Kinet JM, Lutts S (2002) The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant Growth Regul 36:61–70Google Scholar
  9. Barbosa FS, Coelho RD, Maschio R, Lima JGS, Silva EM (2014) Drought resistance of sugar-cane for different levels of water availability in the soil. J Braz Assoc Agric Eng 34:203–210 (Portuguese) Google Scholar
  10. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207Google Scholar
  11. Boaretto LF, Carvalho G, Borgo L, Creste L, Landell MGA, Mazzafera P, Azevedo RA (2014) Water stress reveals differential antioxidant responses of tolerant and non-tolerant sugarcane genotypes. Plant Physiol Biochem 74:165–175PubMedGoogle Scholar
  12. Camargo MS, Amorim L, Gomes Júnior AR (2013) Silicon fertilisation decreases brown rust incidence in sugarcane. Crop Prot 53:72–79Google Scholar
  13. Camargo MS, Korndörfer GH, Wyler P (2014) Silicate fertilization of sugarcane cultivated in tropical soils. Field Crops Res 167:64–75Google Scholar
  14. Camargo MS, Bezerra BLK, Vitti AC, Silva MA, Oliveira AL (2017) Silicon fertilization reduces the deleterious effects of water deficit in sugarcane. J Soil Sci Plant Nutr 17:99–111Google Scholar
  15. Campos PS, Thi ATP (1997) Effect of abscisic acid pretreatment on membrane leakage and lipid composition of Vigna unguiculata leaf discs subjected to osmotic stress. Plant Sci 130:11–18Google Scholar
  16. Chen W, Yao X, Cai K, Chem J (2011) Silicon alleviates drought stress of rice plants by improving plant water status, photosynthesis and mineral nutrient absorption. Biol Trace Elem Res 142:67–76PubMedGoogle Scholar
  17. Cia MC, Guimarães ACR, Medici LO, Chabregas SM, Azevedo RA (2012) Antioxidant responses to water deficit by drought-tolerant and sensitive sugarcane varieties. Ann Appl Biol 161:313–324Google Scholar
  18. Del Longo OT, Gonzáles CA, Pastori FM, Trippi VS (1993) Antioxidant defenses under hyperoxygenic and hyperosmotic conditions in leaves of two lines of maize with differential sensitivity to drought. Plant Cell Physiol 34:1023–1102Google Scholar
  19. Elliott CL, Snyder GH (1991) Autoclave-induced digestion for the colometric determination of silicon in rice straw. J Agric Food Chem 39:1118–1119Google Scholar
  20. Epstein E (2009) Silicon: its manifold roles in plants. Ann Appl Biol. 155:155–160Google Scholar
  21. Ferreira THS, Tsunada MS, Bassi D, Araújo P, Mattiello L, Guidelli GV, Righetto GL, Gonçalves VR, Lakshmanan P, Menossi M (2017) Sugarcane water stress tolerance mechanisms and its implications on developing biotechnology solutions. Front Plant Sci. 8:1–18Google Scholar
  22. Geng A, Wang X, Wu L, Wang F, Wu Z, Yang H, Chen Y, Wen D, Liu X (2018) Silicon improves growth and alleviates oxidative stress in rice seedlings (Oryza sativa L.) by strengthening antioxidant defense and enhancing protein metabolism under arsanilic acid exposure. Ecotoxicol Environ Saf 158:266–273PubMedGoogle Scholar
  23. Gill SS, Tujeja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930PubMedGoogle Scholar
  24. Gong H, Zhu X, Chen K, Wang S, Zhang C (2005) Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci 169:313–321Google Scholar
  25. Gong HJ, Chen KM, Zhao ZG, Chen GC, Zhou WJ (2008) Effects of silicon on defense of wheat against oxidative stress under drought at different developmental stages. Biol Plant 52:592–596Google Scholar
  26. Gunes A, Pilbeam DJ, Inal A, Bagci EG, Coban S (2007) Influence of silicon on antioxidant mechanisms and lipid peroxidation in chickpea (Cicer arietinum L.) cultivars under drought stress. J Plant Interact 2:105–113Google Scholar
  27. Hattori T, Inanaga S, Araki H, Morita S, Luxová M, Lux A (2005) Application of silicon enhanced drought tolerance in Sorghum bicolor. Physiol Plantarum 123:459–466Google Scholar
  28. Havir EA, Mc Hale NA (1987) Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves. Plant Physiol 84:450–455PubMedPubMedCentralGoogle Scholar
  29. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198PubMedPubMedCentralGoogle Scholar
  30. Helaly MN, Rastogi A, Kalaji HM (2017) Regulation and physiological role of silicon in alleviating drought stress of mango. Plant Physiol Biochem 118:31–44PubMedGoogle Scholar
  31. Jain R, Srivastava S, Chandra A (2012) Evaluating sugarcane cultivars for low temperature stress tolerance by electrolyte and phenolic measurements. Trop Agric 89:78–84Google Scholar
  32. Kar M, Mishra D (1976) Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiol 57:315–319PubMedPubMedCentralGoogle Scholar
  33. Kaya C, Tuna L, Higgs D (2006) Effect of silicon on plant growth and mineral nutrition of maize grown under water-stress conditions. J Plant Nutr 29:1469–1480Google Scholar
  34. Keeping MG, Meyer JH, Sewpersad C (2013) Soil silicon amendments increase resistance of sugarcane to stalk borer Eldana saccharina Walker (Lepidoptera: Pyralidae) under field conditions. Plant Soil 363:297–318Google Scholar
  35. Kim YH, Khan AL, Waqas M, Lee IJ (2017) Silicon regulates antioxidant activities of crop plants under abiotic-induced oxidative stress: a review. Front Plant Sci 8:1–7Google Scholar
  36. Koshiba T (1993) Cytosolic ascorbate peroxidase in seedlings and leaves of maize (Zea mays). Plant Cell Physiol 34:713–721Google Scholar
  37. Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: measurement and characterisation by UV-VIS. Current protocols in food analytical chemistry. Wiley, Madison, pp F4.3.1–F4.3.8Google Scholar
  38. Ma D, Sun D, Wang C (2016) Silicon application alleviates drought stress in wheat through transcriptional regulation of multiple antioxidant defense pathways. J Plant Growth Regul 35:1–10Google Scholar
  39. Machado RS, Ribeiro RV, Marchiori PER, Machado DFSP, Machado EC, Landell MGA (2009) Biometric and physiological responses to water deficit in sugarcane at different phenological stages. Pesq Agropec Bras 44:1575–1582Google Scholar
  40. Malavolta E, Vitti GC, Oliveira SA (1997) Evaluation of the nutritional status of plants: principles and applications. Potafós, PiracicabaGoogle Scholar
  41. Medeiros DB, Silva EC, Nogueira RJMC, Teixeira MM, Buckeridge MS (2013) Physiological limitations in two sugarcane varieties under water suppression and after recovering. Theor Exper Plant Physiol 25:213–222Google Scholar
  42. Ming DF, Pei ZF, Naeem MS, Gong HJ, Zhou WJ (2012) Silicon alleviates PEG-induced water deficit stress in upland rice seedlings. J Agric Crop Sci 198:14–26Google Scholar
  43. Molinari HBC, Marur CJ, Daros E, Campos MKF, Carvalho JFRP, Bespalhok Filho LFPP, Vieira LGE (2007) Evaluation of the stress-inducible production of proline in transgenic sugarcane (Saccharum spp.): osmotic adjustment, chlorophyll fluorescence and oxidative stress. Physiol Plantarum 130:281–329Google Scholar
  44. Oliveira CMR, Passos R, Andrade FV, Reis ED, Sturm GM, Souza RB (2010) Corrective of the acidity of the soil and levels of humidity in the development and nutrition of the sugarcane. R Bras Ci Agrarias 5:25–31Google Scholar
  45. Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectrometry. Biochem Biophys Acta 975:384–394Google Scholar
  46. Procházková D, Wilhelmová N (2007) Leaf senescence and activities of the antioxidant enzymes. Biol Plantarum 51:401–406Google Scholar
  47. Ramesh P (2000) Effect of different levels of drought during the formative phase on growth parameters and its relationship with dry matter accumulation in sugarcane. J Agron Crop Sci 185:83–89Google Scholar
  48. Ramouthar PV, Caldwell PM, McFarlane SA (2016) Effect of silicon on the severity of brown rust of sugarcane in South Africa. Eur J Plant Pathol 145:53–60Google Scholar
  49. Sales CR, Ribeiro RV, Silveira JA, Machado EC, Martins MO, Lagôa AM (2013) Superoxide dismutase and ascorbate peroxidase improve the recovery of photosynthesis in sugarcane plants subjected to water deficit and low substrate temperature. Plant Physiol Biochem 73:326–336PubMedGoogle Scholar
  50. Shen X, Zhou Y, Duan L, Eneji AE, Li J (2010) Silicon effects on photosynthesis and antioxidant parameters of soybean seedlings under drought and ultraviolet-B radiation. J Plant Physiol 167:1248–1252PubMedGoogle Scholar
  51. Vital CE, Giordano A, Almeida Soares E, Rhys Williams TC, Mesquita RO, Vidigal PMP, Santana Lopes A, Pacheco TG, Rogalski M, Oliveira Ramos HJ, Loureiro ME (2017) An integrative overview of the molecular and physiological responses of sugarcane under drought conditions. Plant Mol Biol 94:577–594PubMedGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  1. 1.Department of Crop Production and BreedingSão Paulo State University (UNESP)BotucatuBrazil
  2. 2.Department of Chemistry and BiochemistrySão Paulo State University (UNESP)BotucatuBrazil
  3. 3.School of Sciences and EngineeringSão Paulo State University (UNESP)TupãBrazil
  4. 4.Polo Centro SulAgência Paulista de Tecnologia dos Agronegócios (APTA)PiracicabaBrazil

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