Metabolomics reveals the drought-tolerance mechanism in wild soybean (Glycine soja)
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Drought stress is the main limiting factor of crop productivity. Wild soybean (Glycine soja) is a fine germplasm resource, which has a high tolerance to adverse environmental conditions. This study aimed to reveal the mechanism responsible for drought tolerance in drought-tolerant wild soybean. Here, the growth parameters and metabolomics of the two wild soybean varieties’ seedlings were analyzed under polyethylene glycol-simulated drought stress using gas chromatography–mass spectrometry. In total, 61 differentially accumulated metabolites were identified in leaves under polyethylene glycol-6000-simulated drought conditions. Compared with common wild soybean, the drought-tolerant wild soybean grew better. A metabolite profiling analysis suggested that the tricarboxylic acid cycle was enhanced in drought-tolerant wild soybean but inhibited in common wild soybean compared with the control group under simulated drought stress. Thus, the accumulation of osmotic compounds and the enhancement of energy and secondary antioxidant metabolism under drought-stress conditions are the mechanisms responsible for drought tolerance in drought-tolerant wild soybean. The results provide an important theoretical basis for utilizing wild soybean resources.
KeywordsDrought stress Metabolomics Osmotic adjustment Plant growth Soybean
This work was supported by the National Natural Science Foundation of China (no. 31870278) and the Collaborative Innovation of Scientific and Technological of Chinese Academy of Agricultural Sciences. We thank Jilin Academy of Agriculture Science for helping. We thank International Science Editing (http://www.internationalscienceediting.com) for editing this manuscript.
- Corso M, Vannozzi A, Maza E, Vitulo N, Meggio F, Pitacco A, Telatin A, D’Angelo M, Feltrin E, Negri AS, Prinsi B, Valle G, Ramina A, Bouzayen M, Bonghi C, Lucchin M (2015) Comprehensive transcript profiling of two grapevine rootstock genotypes contrasting in drought susceptibility links the phenylpropanoid pathway to enhanced tolerance. J Exp Bot 66:5739–5752CrossRefGoogle Scholar
- Edreva A, Velikova V, Tsonev T, Dagnon S, Gürel A, Akta SL, Gesheva E (2008) Stress-protective role of secondary metabolites: diversity of functions and mechanisms. Gen Appl Plant Physiol 34:67–78Google Scholar
- Marcinska I, Czyczyło-Mysza I, Skrzypek E, Filek M, Grzesiak S, Grzesiak MT, Janowiak F, Hura T, Dziurka M, Dziurka K, Nowakowska A, Quarrie SA (2013) Impact of osmotic stress on physiological and biochemical characteristics in drought susceptible and drought-resistant wheat genotypes. Acta Physiol Plant 35:451–461CrossRefGoogle Scholar
- Moschen S, Rienzo JAD, Higgins J, Tohge T, Watanabe M, González S, Rivarola M, García-García F, Dopazo J, Esteban HH, Hoefgen R, Fernie AR, Paniego N, Fernández P, Heinz RA (2017) Integration of transcriptomic and metabolic data reveals hub, transcription factors involved in drought stress response in sunflower (Helianthus annuus L.). Plant Mol Biol 94:1–16CrossRefGoogle Scholar
- Nakabayashi R, Yonekurasakakibara K, Urano K, Suzuki M, Yamada Y, Nishizawa T, Matsuda F, Kojima M, Sakakibara H, Shinozaki K, Michael AJ, Tohge T, Yamazaki M, Saito K (2014) Enhancement of oxidative and drought tolerance in arabidopsis by overaccumulation of antioxidant flavonoids. Plant J 77:367–379CrossRefGoogle Scholar
- Piasecka A, Sawikowska A, Kuczyńska A, Ogrodowicz P, Mikołajczak K, Krystkowiak K, Gudys K, Guzy-Wrobelska J, Krajewski P, Kachlicki P (2017) Drought-related secondary metabolites of barley (Hordeum vulgare L.) leaves and their metabolomic quantitative trait loci. Plant J 89:898–913CrossRefGoogle Scholar
- Shao S, Li MX, Yang DS, Zhang J, Shi LX (2016) The physiological variations of adaptation mechaniam in Glycine soja seedlings under saline and alkaline stresses. Pak J Bot 48:2183–2193Google Scholar
- Yokoi S, Bressan RA, Hasegawa PM (2002) Salt stress tolerance of plants. JIRCAS Working Report, pp 25–33Google Scholar
- Zhang M, Barg R, Yin MG, Gueta-Dahan Y, Leikin-Frenkel A, Salts Y, Shabtai S, Ben-Hayyim G (2010) Modulated fatty acid desaturation via overexpression of two distinct omega-3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants. Plant J 44:361–371CrossRefGoogle Scholar