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Sulfur-mediated control of salinity impact on photosynthesis and growth in mungbean cultivars screened for salt tolerance involves glutathione and proline metabolism, and glucose sensitivity

  • Sofi J. Hussain
  • Asim Masood
  • Naser A. Anjum
  • Nafees A. KhanEmail author
Original Article
  • 37 Downloads

Abstract

Two mungbean (Vigna radiata L.) cultivars, namely, Punt Mung (salt-tolerant) and Samrat (salt-sensitive), were tested for their response to salinity (50 mM NaCl) and sulfur (1.0 mM SO42− and 2.0 mM SO42−) supplied alone or in combination. Compared to Punt Mung, the extent of 50 mM NaCl-accrued decreases in leaf gas exchange parameters (net photosynthetic rate, intercellular CO2, stomatal conductance, intrinsic water-use efficiency and Rubisco activity), stomatal behavior (frequency and diameter), and plant dry mass was higher in Samrat. However, contents of proline and reduced glutathione (GSH), and the activity of glutathione reductase (GR) and nitrate reductase (NR) were higher in Punt Mung. Notably, the salt-sensitive Samrat exhibited the higher content of glucose and activity of proline oxidase under 50 mM NaCl exposure. The effects of 2.0 mM SO42− in alleviating 50 mM NaCl impact on leaf gas exchange parameters and plant dry mass were more conspicuous in Punt Mung. Overall, SO42− helped Punt Mung to maintain higher cellular levels of GSH and proline; a higher activity of GR and NR; decreased activity of proline oxidase, lower content of glucose, and controlled stomatal behavior. Eventually, SO42−-mediated aforesaid changes culminated into a higher tolerance of Punt Mung to 50 mM NaCl stress when compared to Samrat. Thus, the strategy of sulfur-mediated control of salinity in V. radiata may be adopted for salinity prone areas.

Keywords

Glucose Glutathione Leaf gas exchange Mungbean Proline Salinity Sulfur 

Notes

Acknowledgements

SJH sincerely would like to acknowledge the financial support rendered by UGC in the form of UGC non-NET Fellowship. The research of NAK is supported by the Department of Biotechnology (DBT), New Delhi under the DBT-BUILDER program (No. BT/PR4872/INF/22/150/2012).

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  • Sofi J. Hussain
    • 1
  • Asim Masood
    • 1
  • Naser A. Anjum
    • 1
  • Nafees A. Khan
    • 1
    Email author
  1. 1.Plant Physiology and Biochemistry Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia

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