The effect of plant growth regulators on recovery of wheat physiological and yield-related characteristics at booting stage following chilling stress
- 36 Downloads
Chilling stress has a strong negative impact on the growth and development of winter wheat (Triticum aestivum L.). To investigate the recovery of physiological function and yield formation by plant growth regulators following chilling stress, we performed low-temperature phytotron experiments at the booting stage, and sprayed 6-benzylamino adenine (6-BA), salicylic acid (SA), brassinolide (BR) and abscisic acid (ABA) after chilling stress. Plant growth regulators significantly enhanced SPAD value and net photosynthetic rate (Pn) in flag leaves following chilling stress (p < 0.05). Compared with the control group sprayed with distilled water, stomatal conductance (Gs) and transpiration rate (Tr) increased, while intercellular carbon dioxide concentration (Ci) decreased. In addition, the concentration of malondialdehyde (MDA) was significantly decreased, and the activities of superoxide dismutase (SOD) and peroxidase (POD) were enhanced (p < 0.05). Plant growth regulators also increased the grain filling rate during the 14 days after anthesis, thereby increasing grain weight. The grain number per spike, 1000-kernel weight, and yield per plant after harvest were also significantly enhanced (p < 0.05). Thus, spraying plant growth regulators at the booting stage relieved the adverse effects on physiological activity in wheat flag leaves caused by chilling stress, and 6-BA and SA were particularly effective.
KeywordsWheat Plant growth regulator Chilling stress Booting stage Yield
Intercellular CO2 concentration
Reactive oxygen species
Soil and plant analyzer development
This work was supported by the National Key Research and Development Program of China (Grant no. 2017YFD0300205, 2018YFD0300902), the National Natural Science Foundation of China (Grant no. 31801285), the University Natural Science Research Project of Anhui Province (Grant no. KJ2017A141) and the National College Students’ Innovative Entrepreneurship Training Program (Grant no. 201710364025).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Bao YX, Wang Y, Gao P, Shen SH (2012) Occurrence pattern on spring frost injury of winter wheat in Jiangsu province and its climatic risk division. Chin J Agrometeorol 33:134–141. https://doi.org/10.3969/j.issn.1000-6362.2012.01.022 (in Chinese with English abstract) CrossRefGoogle Scholar
- Guo WL, Chen RG, Gong ZH, Yin YX, Ahmed SS, He YM (2012) Exogenous abscisic acid increases antioxidant enzymes and related gene expression in pepper (Capsicum annuum) leaves subjected to chilling stress. Genet Mol Res 11:4063–4080. https://doi.org/10.4238/2012.September.10.5 CrossRefPubMedGoogle Scholar
- Ji XM, Dong BD, Shiran B, Talbot MJ, Edlington JE, Trijntje H, White RG, Gubler F, Dolferus R (2011) Control of ABA catabolism and ABA homeostasis is important for reproductive stage stress tolerance in cereals. Plant Physiol 156:647–662. https://doi.org/10.1104/pp.111.176164 CrossRefPubMedPubMedCentralGoogle Scholar
- Kosová K, Prásil IT, Vítámvás P, Dobrev P, Motyka V, Floková K, Novák O, Turecková V, Rolcik J, Pesek B, Trávnicková A, Gaudinová A, Galiba G, Janda T, Vlasáková E, Prásilová P, Vanková R (2012) Complex phytohormone responses during the cold acclimation of two wheat cultivars differing in cold tolerance, winter Samanta and spring Sandra. J Plant Physiol 169:567–576. https://doi.org/10.1016/j.jplph.2011.12.013 CrossRefPubMedGoogle Scholar
- Kosová K, Vítámvás P, Planchon S, Renaut J, Vanková R, Prášil IT (2013) Proteome analysis of cold response in spring and winter wheat (Triticum aestivum) crowns reveals similarities in stress adaptation and differences in regulatory processes between the growth habits. J Proteome Res 12:4830–4845. https://doi.org/10.1021/pr400600g CrossRefPubMedGoogle Scholar
- Li X, Cai J, Liu F, Dai TB, Cao WX, Jiang D (2014) Cold priming drives the sub-cellular antioxidant systems to protect photosynthetic electron transport against subsequent low temperature stress in winter wheat. Plant Physiol Bioch 82:34–43. https://doi.org/10.1016/j.plaphy.2014.05.005 CrossRefGoogle Scholar
- Wang Q, Huang ZL, Zhang WJ, Liu MC, Zhou XN, Liu L (2015) Effect of spraying 6-BA and Xin Meizhouxing on photosynthesis and yield of wheat after rice under low temperature stress. J Triticeae Crops 35:1269–1274 (in Chinese with English abstract) Google Scholar
- Zheng YH, Li X, Li YG, Miao BH, Xua H, Simmons M, Yang XH (2012) Contrasting responses of salinity-stressed salt-tolerant and intolerant winter wheat (Triticum aestivum L.) cultivars to ozone pollution. Plant Physiol Bioch 52:169–178. https://doi.org/10.1016/j.plaphy.2012.01.007 CrossRefGoogle Scholar