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Soluble sugars, phenolic acids and antioxidant capacity of grape berries as affected by iron and nitrogen

  • Rouhollah KarimiEmail author
  • Mohammad Koulivand
  • Nathalie Ollat
Original Article
  • 24 Downloads

Abstract

Foliar nutrition is one of the effective cultural practices in vineyards. In this research, the effect of iron chelate (Fe-EDDHA) and urea, each in three levels of 0, 0.5 and 1%, was evaluated with an ANOVA completely randomized block in commercial vineyard (cv “Sultana”) located in Bahareh village of Malayer city (Iran). Vines were sprayed in three stages: a week before bloom (8 June), 2 weeks after bloom (29 June) and 5 weeks after bloom (20 July) during the growth seasons in 2015 and 2016. The grapes harvesting was done in mid-September according to the maturity level of untreated vines. In comparison with the other treatments, moderate levels (0.5%) of fertilizers allow to reach the highest glucose and sucrose concentration at harvest. Foliar spray of high iron chelate doses in combined with 0.5% urea caused a considerable increase in berries putrescine and spermine concentration. However, combination effects of urea and Fe-EDDHA with moderate level (0.5%) were the most efficient for spermidine accumulation of ‘Sultana’ grapevine. For the moderate levels (Fe-EDDHA 0.5%) of fertilizers treatment, most phenolic acids and anthocyanidins reached a peak, and the highest free radical scavenging capacities (DPPH) of grape samples were achieved. The activity superoxide dismutase, guaiacol peroxidase, catalase and ascorbate peroxidase increased with moderate levels of Fe-EDDHA in combination with high levels of urea treatments. However, the maximum glutathione reductase was obtained with 1% urea in combination with Fe-EDDHA at 1% concentrations. Altogether, data showed that iron and nitrogen are highly efficient to manage quality and nutritional potential of grape berries.

Keywords

Anthocyanidins Glucose Glutathione reductase Grapes Nitrogen Nutrition 

Notes

Acknowledgements

Funding was provided by Malayer University (Grant no. 84.5-289).

Supplementary material

11738_2019_2910_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 15 kb)

References

  1. Abd El-Razek E, Treutter D, Saleh MMS, El-Shammaa M, Fouad AA, Abdel-Hamid N (2011) Effect of nitrogen and potassium fertilization on productivity and fruit quality of ‘Crimson seedless’ grape. Agric Biol J North Am 2:330–340CrossRefGoogle Scholar
  2. Abdel-Salam MM (2016) Effect of foliar application of salicylic acid and micronutrients on the berries quality of ‘Bezel Naka’ local grape cultivar. Sciences 6:178–188Google Scholar
  3. Ahmed FF, Akl AM, El-Morsy FM (1997) Yield and quality of ‘Banaty’grapes in response to spraying iron and zinc. HortScience 32:516D–516CrossRefGoogle Scholar
  4. Ali K, Maltese F, Choi YH, Verpoorte R (2010) Metabolic constituents of grapevine and grape-derived products. Phytochem Rev 9:357–378CrossRefGoogle Scholar
  5. Álvarez-Fernández A, Paniagua P, Abadía J, Abadía A (2003) Effects of Fe deficiency chlorosis on yield and fruit quality in peach (Prunus persica L. Batsch). J Agric Food Chem 51:5738–5744CrossRefGoogle Scholar
  6. Àlvarez-Fernàndez A, Abadía J, Abadía A (2006) Iron deficiency, fruit yield and fruit quality. In: Barton LL, Abadía J (eds) Iron nutrition in plants and rhizospheric microorganisms. Springer, Dordrecht, pp 85–101CrossRefGoogle Scholar
  7. Askary M, Amirjani MR, Saberi T (2017) Comparison of the effects of nano-iron fertilizer with iron-chelate on growth parameters and some biochemical properties of Catharanthus roseus. J Plant Nutr 40:974–982CrossRefGoogle Scholar
  8. Bacha MA, Sabbah SM, El-Hamady MA (1995) Effect of foliar applications of iron, zinc and manganese on yield, berry quality and leaf mineral composition of Thompson Seedless and Roumy Red grape cultivars. Alex J Agric Res 40:315–331Google Scholar
  9. Bavaresco L, Pezzutto S, Ragga A, Ferrari F, Trevisan M (2001) Effect of nitrogen supply on trans-resveratrol concentration in berries of Vitis vinifera L. cv. Cabernet Sauvignon. Vitis 40:229–230Google Scholar
  10. Bavaresco L, de Macedo MIVZ, Gonçalves B, Civardi S, Gatti M, Ferrari F (2010) Effects of traditional and new methods on overcoming lime-induced chlorosis of grapevine. Am J Enol Vitic 61:186–190Google Scholar
  11. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287CrossRefGoogle Scholar
  12. Bell SJ, Henschke PA (2005) Implications of nitrogen nutrition for grapes, fermentation and wine. Aust J Grape Wine R 11:242–295CrossRefGoogle Scholar
  13. Bergmeyer N (1970) Methoden der Enzymatischen Analyse, vol 1. Akademie, Berlin, pp 636–647Google Scholar
  14. Bertamini M, Nedunchezhian N (2005) Grapevine growth and physiological responses to iron deficiency. J Plant Nutr 28:737–749CrossRefGoogle Scholar
  15. Bozin B, Mimica-Dukic N, Samojlik I, Goran A, Igic R (2008) Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chem 111:925–929CrossRefGoogle Scholar
  16. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  17. Canoura C, Kelly MT, Ojeda H (2018) Effect of irrigation and timing and type of nitrogen application on the biochemical composition of Vitis vinifera L. cv. Chardonnay and Syrah grape berries. Food Chem 241:171–181CrossRefGoogle Scholar
  18. Castellarin SD, Bavaresco L, Falginella L, Gonçalves MVZ, Di Gaspero G (2013) Phenolics in grape berry and key antioxidants. Int J Mol Sci 14:18711–18739CrossRefGoogle Scholar
  19. Celette F, Findeling A, Gary C (2009) Competition for nitrogen in an unfertilized intercropping system: the case of an association of grapevine and grass cover in a Mediterranean climate. Eur J Agron 30:41–51CrossRefGoogle Scholar
  20. Comis DB, Tamayo DM, Alonso JM (2001) Determination of monosaccharaides in cider by reversed-phase liquid chromatography. Anal Chim Acta 436:173–178CrossRefGoogle Scholar
  21. Curie C, Briat JF (2003) Iron transport and signaling in plants. Annu Rev Plant Biol 54:183–206CrossRefGoogle Scholar
  22. Curie C, Cassin G, Couch D, Divol F, Higuchi K, Le Jean M, Mari S (2008) Metal movement within the plant: contribution of nicotianamine and yellow stripe 1-like transporters. Ann Bot 103:1–11CrossRefGoogle Scholar
  23. Daglia M, Di Lorenzo A, Nabavi SF, Talas ZS, Nabavi SM (2014) Polyphenols: well beyond the antioxidant capacity: gallic acid and related compounds as neuroprotective agents: you are what you eat! Curr Pharm Biotechnol 15:362–372CrossRefGoogle Scholar
  24. Dai ZW, Ollat N, Gomès E, Decroocq S, Tandonnet JP, Bordenave L, Pieri P, Hilbert G, Kappel C, van Leeuwen C, Vivin P (2011) Ecophysiological, genetic, and molecular causes of variation in grape berry weight and composition: a review. Am J Enol Vitic 62:413–425CrossRefGoogle Scholar
  25. Delgado R, Martín P, del Álamo M, González MR (2004) Changes in the phenolic composition of grape berries during ripening in relation to vineyard nitrogen and potassium fertilisation rates. J Sci Food Agric 84:623–630CrossRefGoogle Scholar
  26. Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133:21–25CrossRefGoogle Scholar
  27. Garde-Cerdán T, Portu J, López R, Santamaría P (2015) Effect of foliar applications of proline, phenylalanine, urea, and commercial nitrogen fertilizers on stilbene concentrations in Tempranillo musts and mines. Am J Enol Vitic 66:4CrossRefGoogle Scholar
  28. Gutiérrez-Gamboa G, Garde-Cerdán T, Gonzalo-Diago A, Moreno-Simunovic Y, Martínez-Gil AM (2017) Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard. LWT Food Sci Technol 75:147–154CrossRefGoogle Scholar
  29. Habran A, Commisso M, Helwi P, Hilbert G, Negri S, Ollat N, Gomès E, van Leeuwen C, Guzzo F, Delrot S (2016) Roostocks/scion/nitrogen interactions affect secondary metabolism in the grape berry. Front Plant Sci 7:1134CrossRefGoogle Scholar
  30. Herzog V, Fahimi HD (1973) Determination of the activity of peroxidase. Anal Biochem 55:554–562CrossRefGoogle Scholar
  31. Hufnagel JC, Hofmann T (2008) Quantitative reconstruction of the nonvolatile sensometabolome of a red wine. J Agric Food Chem 56:9190–9199CrossRefGoogle Scholar
  32. Jackson DI, Lombard PB (1993) Environmental and management practices affecting grape composition and wine quality-a review. Am J Enol Vitic 44:409–430Google Scholar
  33. Jiménez S, Gogorcena Y, Hévin C, Rombolà AD, Ollat N (2007) Nitrogen nutrition influences some biochemical responses to iron deficiency in tolerant and sensitive genotypes of Vitis. Plant Soil 290:343–355CrossRefGoogle Scholar
  34. Karimi R (2017) Potassium-induced freezing tolerance is associated with endogenous abscisic acid, polyamines and soluble sugars changes in grapevine. Sci Hortic 215:184–194CrossRefGoogle Scholar
  35. Keller M (2015) The science of grapevines: anatomy and physiology, 2nd edn. Academic Press, Burlington, p 400Google Scholar
  36. Keller M, Kummer M, Vasconcelos MC (2001) Reproductive growth of grapevines in response to nitrogen supply and rootstock. Aust J Grape Wine R 7:12–18CrossRefGoogle Scholar
  37. Koponen J, Happonen A, Mattila P, Torronen R (2007) Contents of anthocyanins and ellagitannins in foods consumed in Finland. J Agric Food Chem 55:1612–1619CrossRefGoogle Scholar
  38. Lacroux F, Tregoat O, Van Leeuwen C, Pons A, Tominaga T, Lavigne-Cruège V, Dubourdieu D (2008) Effect of foliar nitrogen and sulphur application on aromatic expression of Vitis vinifera L. cv. Sauvignon blanc. J Int Sci Vigne Vin 42:125–132Google Scholar
  39. Lasa B, Menendez S, Sagastizabal K, Cervantes MEC, Irigoyen I, Muro J, Ariz I (2012) Foliar application of urea to Sauvignon Blanc and Merlot vines: doses and time of application. Plant Growth Regul 67:73–81CrossRefGoogle Scholar
  40. Marschner H (2011) Marschner’s mineral nutrition of higher plants, 3rd edn. Academic Press, London, pp 178–189Google Scholar
  41. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
  42. Nezami MT (2012) The effects of foliar applications of nitrogen, boron, and zinc on the fruit setting and the quality of almonds. Life Sci J 9:1979–1989Google Scholar
  43. OIV Statistical Report on World Vitiviniculture (2017) International Organization of vine and wine (OIV). http://www.oiv.int
  44. Panagiotis MN, Aziz A, Kalliopie RAA (2012) Polyamines and grape berry development. In: Hernâni G, Manuela C, Serge D (eds) The biochemistry of the grape berry. Bentham Science Publishers, USA, pp 137–159CrossRefGoogle Scholar
  45. Ranieri A, Castagna A, Baldan B, Soldatini GF (2001) Iron deficiency differently affects peroxidase isoforms in sunflower. J Exp Bot 52:25–35CrossRefGoogle Scholar
  46. Rombolà AD, Brüggemann W, Tagliavini M, Marangoni B, Moog PR (2000) Iron source affects iron reduction and re-greening of kiwifruit (Actinidia deliciosa) leaves. J Plant Nutr 23:1751–1765CrossRefGoogle Scholar
  47. Roosta HR, Mohsenian Y (2012) Effects of foliar spray of different Fe sources on pepper (Capsicum annum L.) plants in aquaponic system. Sci Hortic 146:182–191CrossRefGoogle Scholar
  48. Salih HO (2013) Effect of Foliar Fertilization of Fe, B and Zn on nutrient concentration and seed protein of Cowpea Vigna unguiculata. J Agric Vet Sci 6:42–46Google Scholar
  49. Schreiner RP, Scagel CF, Baham J (2006) Nutrient uptake and distribution in a mature “Pinot noir” vineyard. HortScience 41:336–345CrossRefGoogle Scholar
  50. Shi P, Li B, Chen H, Song C, Meng J, Xi Z, Zhang Z (2017) Iron supply affects anthocyanin content and related gene expression in berries of Vitis vinifera cv. Cabernet Sauvignon. Molecules 22:283CrossRefGoogle Scholar
  51. Shin KS, Chakrabarty D, Paek KY (2002) Sprouting rate, change of carbohydrate contents and related enzymes during cold treatment of Lily bulblets regenerated in vitro. Sci Hortic 96:195–204CrossRefGoogle Scholar
  52. Sing S (2006) Grapevine nutrition literature review. Cooperative Research Centre for Viticulture, RenmarkGoogle Scholar
  53. Smolders AJP, Hendriks RJJ, Campschreur HM, Roelofs JGM (1997) Nitrate induced iron deficiency iron deficiency chlorosis in Juncus acutiflorus. Plant Soil 196:37–45CrossRefGoogle Scholar
  54. Soubeyrand E, Basteau C, Hilbert G, van Leeuwen C, Delrot S, Gomès E (2014) Nitrogen supply affects anthocyanin biosynthetic and regulatory genes in grapevine cv. Cabernet-Sauvignon berries. Phytochemistry 103:38–49CrossRefGoogle Scholar
  55. Stockert CM, Bisson LF, Adams DO, Smart DR (2013) Nitrogen status and fermentation dynamics for Merlot on two rootstocks. Am J Enol Vitic 64:195–202CrossRefGoogle Scholar
  56. Vekiari SA, Panagou E, Mallidis C (2008) Extraction and determination of ellagic acid content in chestnut bark and fruit. Food Chem 110:1007–1011CrossRefGoogle Scholar
  57. Walter H, Geuns J (1987) High speed HPLC analysis of polyamines in plant tissues. Plant Physiol 83:2–234CrossRefGoogle Scholar
  58. Zhu XF, Wang B, Song WF, Zheng SJ, Shen RF (2016) Putrescine alleviates iron deficiency via NO-dependent reutilization of root cell-wall Fe in Arabidopsis. Plant Physiol 170:558–567CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  1. 1.Department of Landscape Engineering, Faculty of AgricultureMalayer UniversityMalayerIran
  2. 2.Grapevine Production and Genetic Improvement Department, Research Institute for Grapes and RaisinMalayer UniversityMalayerIran
  3. 3.INRA, Université de Bordeaux, ENITAB, ISVV, UMR 1287 Ecophysiologie et Génomique Fonctionnelle de la VigneVillenave d’OrnonFrance

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