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Somatic embryogenesis-mediated plant regeneration of Eclipta alba (L.) Hassk. and its conservation through synthetic seed technology

  • Umme Salma
  • Suprabuddha KunduEmail author
  • Md. Nasim Ali
  • Nirmal Mandal
Original Article
  • 12 Downloads

Abstract

Plant regeneration by means of somatic embryogenesis has been standardized for the first time in Eclipta alba (L.) Hassk. Explants like nodal segment, shoot tip, and leaf were tested on Murashige and Skoog medium supplemented with picloram, 2,4-dichlorophenoxyacetic acid, or α-naphthalene acetic acid for callus induction. Nodal segment exhibited the maximum response (92.80%) to callus induction on culture medium-containing 2.0 mg l−1 picloram. Furthermore, highest rate (88.40%) and number (34.83) of somatic embryo induction were found on medium fortified with 2.0 mg l−1 picloram, 0.5 mg l−1 thidiazuron, and 0.25 mg l−1 abscisic acid. Influence of abscisic acid on the somatic embryo regeneration was also tested with the highest frequency (95.50%) attained on medium-containing 0.75 mg l−1 N6-benzyladenine and 0.5 mg l−1 abscisic acid. Somatic embryo of torpedo stage was efficiently encapsulated with 2.5% sodium alginate and 75 mM calcium chloride, and exhibited 93.33% germination rate. The study revealed that storage at low temperature (8 °C) gave superior results where 86.67% of the synthetic seeds sprouted even after 60 days with a decline to 46.67% in 90 days post-storage. The rate of germination at 25 °C had been constantly low and failed to sprout after 75 days. The acclimatization of the regenerated plantlets was successfully taken place in garden soil, sand, and vermicompost in the ratio of 1:1:1 with 95% survival rate.

Keywords

Abscisic acid Eclipta alba Somatic embryogenesis Synthetic seed 

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

ABA

Abscisic acid

BA

N6-benzyladenine

CC

Calcium chloride

MS

Murashige and Skoog (1962)

Lf

Leaf

NS

Nodal segment

NAA

α-Naphthalene acetic acid

Picloram

4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid

PGR

Plant growth regulator

SA

Sodium alginate

SE

Somatic embryo

ST

Shoot tip

TDZ

N-Phenyl-N′-(1,2,3-thidiazol-5-yl) urea or Thidiazuron

Notes

Acknowledgements

Authors acknowledge the laboratory, as well as library assistance from the Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

11738_2019_2898_MOESM1_ESM.docx (28 kb)
Supplementary material 1 (DOCX 27 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  1. 1.Department of Agricultural Biotechnology, Faculty of AgricultureBidhan Chandra Krishi ViswavidyalayaNadiaIndia

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