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Physiological introspection into differential drought tolerance in rice cultivars of North East India

  • Smita Sahoo
  • Bedabrata Saha
  • Jay Prakash Awasthi
  • Takhellambam Omisun
  • Pankaj Borgohain
  • Safiqul Hussain
  • Jogeswar Panigrahi
  • Sanjib Kumar PandaEmail author
Original Article
  • 88 Downloads

Abstract

Drought is a vice to world crop production, exponentially enhanced by global climate change. Rice, a basic food crop for a major chunk of world populace, is largely affected by environmental challenges such as drought, salinity and heavy metal. This study brings to limelight differential drought tolerance capacity of rice varieties indigenous to North East India, a hot bed of indica rice diversity. Initial screening of rice varieties were performed through physiological dose-dependent studies under PEG (0%, 10%, and 20% which is equal to osmotic potential values of 0.001, 0.54 and 1.09 MPa, respectively), induced drought stress for three time intervals of 1, 3 and 5 days. Hierarchical clustering of the parameters on which the cultivars were analysed revealed Tampha and KMJ 1-12-3 to be relatively more tolerant whereas Chandan and Ketaki Joha as the sensitive ones. Biochemical studies for hydrogen peroxide (H2O2), proline, lipid peroxidation (MDA), lipoxygenase (LOX), superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR) were then performed on two cultivars (Tampha and Chandan) to elucidate the differential tolerance to drought, focusing on anti-oxidative response mechanism. The biochemical fingerprint showed significantly lower accumulation of H2O2 and MDA; higher accumulation of proline; and higher activity of SOD, CAT, DHAR, MDHAR and GR in the tolerant variety Tampha when compared to Chandan. In addition, alteration of chlorophyll fluorescence due to stress was also monitored to ascertain the variation in photosynthetic efficiency between the tolerant and sensitive cultivars. Tampha showed better photosynthetic activity in comparison to Chandan as quantified by measuring chlorophyll fluorescence. This manuscript thus throws new light into the drought stress response of the varieties from North East India with global implications.

Keywords

Oryza sativa Drought Abiotic stress Tolerance Physiology 

Notes

Acknowledgements

We highly acknowledge Regional Agricultural Research Station (RARS), Akbarpur (Karimganj), Assam, India; Regional Rainfed Lowland Rice Research Station (RRLRRS) Gerua, Assam, India; and Central Agriculture University (CAU), Imphal, Manipur, India for supplying us with valuable rice seeds.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interest.

Supplementary material

11738_2019_2841_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 KB)
11738_2019_2841_MOESM2_ESM.xlsx (25 kb)
Supplementary material 2 (XLSX 25 KB)
11738_2019_2841_MOESM3_ESM.docx (164 kb)
Supplementary material 3 (DOCX 164 KB)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  • Smita Sahoo
    • 1
  • Bedabrata Saha
    • 1
  • Jay Prakash Awasthi
    • 1
  • Takhellambam Omisun
    • 1
  • Pankaj Borgohain
    • 1
  • Safiqul Hussain
    • 2
  • Jogeswar Panigrahi
    • 3
  • Sanjib Kumar Panda
    • 1
    Email author
  1. 1.Plant Molecular Biotechnology Laboratory, Department of Life Science and BioinformaticsAssam UniversitySilcharIndia
  2. 2.Regional Agricultural Research StationAssam Agricultural UniversityKarimganjIndia
  3. 3.Department of BiotechnologyCentral University of RajasthanAjmerIndia

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