Comparison of physiological and methylational changes in resynthesized Brassica napus and diploid progenitors under drought stress

  • Jinjin Jiang
  • Yi Yuan
  • Shuang Zhu
  • Tingting Fang
  • Liping Ran
  • Jian Wu
  • Youping WangEmail author
Original Article


Brassica napus is a polyploid of certain research and economical value. Resynthesizing B. napus with diploid B. rapa and B. oleracea is essential for Brassica research because of the limited genetic background of B. napus. Considering that polyploids possess better agronomic traits and resistance compared with the corresponding diploids, we investigated drought tolerance after polyploidization of B. napus and revealed the epigenetic differences between polyploids and diploids. After drought stress, B. rapa and first-generation of synthesized hybrids (F1) were more wilted than B. oleracea and F2–F4 generations. However, the relative water content and water retention in F1 were better than others after drought stress. The increased number of partially opened and closed stomata in F1 was not as significant as that in F2 and F3, but stomata density in F1 was lower than F2, and the stomatal size in F1 was significantly reduced than F3. Physiological parameters varied among different generations of B. napus and diploid parents, and most of these parameters in hybrids were higher than B. rapa and lower than B. oleracea. However, the peroxidase activity in F3 and F4 was significantly higher than both parents, and the malondialdehyde content in F3 and F4 was lower than both parents, indicating that F3 and F4 might be more adaptive to oxidative stresses than other generations. DNA methylation level was decreased in F2 and F3 compared with F1, and then increased in F4. Methylation-sensitive amplified polymorphism analysis revealed that DNA methylation and demethylation broadly happened after drought stress. The methylation and demethylation level was F1 > F4 > B. oleracea > F2 > F3 > B. rapa and B. rapa > F4 > F3 > F2 > B. oleracea > F1, respectively. The epigenetic changes under drought stress might be related to the different stress tolerances during B. napus polyploidization.


Resynthesized Brassica napus Drought stress Antioxidants DNA methylation 



Methyltransferase chromomethylase 3




Methyltransferase 1


Murashige and Skoog


Methylation-sensitive amplified polymorphism


Nitrotetrazolium blue chloride


Phosphate-buffered saline


Polyethylene glycol




Polyvinyl pyrrolidone


Quantitative reverse transcriptase polymerase chain reaction


Relative water content


Relative water retention


Superoxide dismutase


Thiobarbituric acid


Trichloroacetic acid



This work was supported by the National Natural Science Foundations (31771824, 31330057, 31771825, 31401414), National Key Research and Development Program of China (2016YFD0101000, 2016YFD0102000), China Postdoctoral Science Foundation (2015T80591, 2014M561719), Jiangsu Postdoctoral Science Foundation (1401078B), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Supplementary material

11738_2019_2837_MOESM1_ESM.xlsx (11 kb)
Supplementary material 1 (XLSX 11 KB)


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  • Jinjin Jiang
    • 1
  • Yi Yuan
    • 1
  • Shuang Zhu
    • 1
  • Tingting Fang
    • 1
  • Liping Ran
    • 1
  • Jian Wu
    • 1
  • Youping Wang
    • 1
    Email author
  1. 1.Jiangsu Provincial Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhouChina

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