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Acta Physiologiae Plantarum

, 41:30 | Cite as

The dynamic changes of catechins and related genes in tea (Camellia sinensis) flowers

  • Litao Sun
  • Yu Wang
  • Zhaotang DingEmail author
  • Feng Liu
Original Article
  • 16 Downloads

Abstract

As the main components in tea flowers, catechins are accumulated at each stage of tea flowering. However, little information on the accumulation mechanism of catechins during tea flowering is available. Here, we analyzed the accumulation of catechins in tea flowers as well as the expressions of related genes during tea flowering. The results indicated that 99 unigenes and 269 transcription factors (TFs) were assigned to the flavonoid biosynthesis pathway, and the contents of catechins differed significantly at different flowering stages, except for epigallocatechin. A holistic analysis between the contents of catechins and the expressions of catechin-related genes suggested that phenylalanine ammonia lyase (PAL1, Unigene23632), 4-coumaroyl-CoA ligase (4CL1, Unigene40857), chalcone synthase (CHS2, CL5310.Contig3), flavonoid 3′-hydroxylase (F3′H1, CL491.Contig2), and flavonol synthase (FLS, Unigene33148) were the critical genes in regulating the biosynthesis of catechins. Thereinto, the CHS2 and FLS that highly expressed at the early flowering stage showed positive correlations with the contents of catechin, epigallocatechin gallate, epicatechin gallate, and total catechins. In addition, the 4CL1, F3′H1, and PAL1 that highly expressed at later flowering stage showed negative correlations with the contents of total catechins. Moreover, the MYB, bHLH, NAC, WRKY, and MADS could be the major TFs implicated in the biosynthesis of catechins in tea flowers. Among them, MYB44, WRKY7, and WRKY17 were special TFs regulating catechins biosynthesis in tea flowering. Overall, this study first described the function of genes related to catechins biosynthesis in tea flowers, and the data may serve as valuable resources for studying the molecular mechanisms of catechins biosynthesis during tea flowering.

Keywords

Camellia sinensis (L.) O. Kuntze Flower Catechins Transcription factors 

Abbreviations

KEGG

Kyoto encyclopedia of genes and genomes

DEGs

Differentially expressed genes

qRT-PCR

Quantitative real-time PCR

TFs

Transcription factors

ECG

Epicatechin gallate

EGC

Epigallocatechin

EC

Epicatechin

GC

Gallocatechin

C

Catechin

EGCG

Epigallocatechin gallate

PAL

Phenylalanine ammonia lyase

C4H

Cinnamate 4-hydroxylase

F3′5′H

Flavonoid 3′,5′-hydroxylase

CHI

Chalcone isomerase

4CL

4-Coumaroyl-CoA ligase

CHS

Chalcone synthase

F3H

Flavanone 3-hydroxylase

F3′H

Flavonoid 3′-hydroxylase

FLS

Flavonol synthase

FGS

Flavan-3-ol gallate synthase

DFR

Dihydroflavonol 4-reductase

LAR

Leucoanthocyanidin reductase

ANS

Anthocyanidin synthase

ANR

Anthocyanidin reductase

UFGT

UDP-glucose

Notes

Acknowledgements

This work was funded by the Project of Major Agricultural Application Technology Innovation in Shandong Province (2018), the Breeding Project of Shandong Province (2017LZN014), the Technology System of Modern Agricultural Industry in Shandong Province (SDAIT-19-01), and the Special Foundation for Distinguished Taishan Scholar of Shandong Province (No. ts201712057).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  1. 1.Tea Research InstituteQingdao Agricultural UniversityQingdaoChina

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