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Acta Physiologiae Plantarum

, 41:27 | Cite as

Environmental factors’ effect on seed germination and seedling growth of chicory (Cichorium intybus L.) as an important medicinal plant

  • Fatemeh Vahabinia
  • Hemmatollah Pirdashti
  • Esmaeil BakhshandehEmail author
Original Article
  • 27 Downloads

Abstract

Chicory (Cichorium intybus L.; Asteraceae) is a small aromatic and medicinal biennial and perennial herb that is distributed in most parts of Europe and Asia including Iran. However, little information is available about seed germination (SG) and seedling growth of this plant in response to abiotic environmental factors. Therefore, this study aimed to investigate the effect of several environmental factors such as temperature (T), water stress (ψ), salinity, pH and burial depth on SG characteristics of chicory. Results indicated that all studied traits including germination percentage (GP), germination rate (GR), germination uniformity (GU), normal seedling percentage (NSP), root length (RL), shoot length (SL) and seedling dry weight (SDW) are significantly influenced by each environmental factor. Estimated cardinal Ts were 3.5, 28.9 and 40.2 °C for the base, optimum and ceiling T, respectively, with a thermal time 330.2 °C h after fitting a beta model in water. The drought tolerance threshold value was − 0.82 MPa for GP and − 0.75 MPa for NSP. The sensitive of each trait to ψ was ranking RL > SL > GR > SDW > NSP > GP. Increasing salinity level from 0 to 250 mM declined GP, GR, NSP, RL, SL and SDW by 75, 83, 88, 85, 80 and 60%, respectively, and also GU decreased seven times compared with the control. The salt tolerance threshold value was 223 and 194 mM for GP and NSP, respectively. Although chicory seeds were able to germinate at all pH levels (84%, ranged from 2 to 10), they could not produce an equivalent normal seedling in the same condition which indicates that seedling growth is more sensitive to pH relative to SG. The best pH for germination and seedling growth was estimated to be ~ 7 for this plant. Seedling emergence increased by 25% as burial depth increased from 0.5 to 2 cm and then sharply decreased by 87% when reached to 4 cm. The best burial depth ranged from 1 to 2 cm (> 88%) for chicory. Consequently, this information could help us to adequately manage the production of this plant under different environmental factors and also to determine its geographic range expansion in the world.

Keywords

Burial depth Chicory pH Salt stress Temperature Water stress 

Abbreviations

GP

Final germination percentage

GR

Germination rate

GU

Germination uniformity

mM

Millimolar

MPa

Megapascal

NSP

Normal seedling percentage

PEG

Polyethylene glycol

RL

Root length

SDW

Seedling dry weight

SE

Seedling emergence

SG

Seed germination

SL

Shoot length

T

Temperature

Tb

Minimum temperature (base temperature)

Tc

Maximum temperature (ceiling temperature)

To

Optimum temperature

TT

Thermal time

ψ

Water potential

Notes

Acknowledgements

This research is supported by the Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT) and Sari Agricultural Sciences and Natural Resources University (SANRU) research grant. The authors gratefully acknowledge use of the services and facilities of the GABIT during this research and also Dr. Raoudha Abdellaoui for scientific and linguistic revisions.

References

  1. Abdellaoui R, Boughalleb F, Zayoud D, Neffati M, Bakhshandeh E (2019) Quantification of Retama raetam seed germination response to temperature and water potential using hydrothermal time concept. Environ Exp Bot 157:211–216CrossRefGoogle Scholar
  2. Alvarado V, Bradford K (2002) A hydrothermal time model explains the cardinal temperatures for seed germination. Plant Cell Environ 25:1061–1069CrossRefGoogle Scholar
  3. Amini V, Zaefarian F, Rezvani M (2015) Interspecific variations in seed germination and seedling emergence of three Setaria species. Braz J Bot 38:539–545CrossRefGoogle Scholar
  4. Atashi S, Bakhshandeh E, Zeinali Z, Yassari E, Teixeira da Silva JA (2014) Modeling seed germination in Melisa officinalis L. in response to temperature and water potential. Acta Physiol Plant 36:605–611CrossRefGoogle Scholar
  5. Atashi S, Bakhshandeh E, Mehdipour M, Jamali M, Teixeira da Silva JA (2015) Application of a hydrothermal time seed germination model using the Weibull distribution to describe base water potential in zucchini (Cucurbita pepo L.). J Plant Growth Regul 34:150–157CrossRefGoogle Scholar
  6. Bakhshandeh E, Gholamhossieni M (2018) Quantification of soybean seed germination response to seed deterioration under PEG-induced water stress using hydrotime concept. Acta Physiol Plant 40:126CrossRefGoogle Scholar
  7. Bakhshandeh E, Atashi S, Hafez-Nia M, Pirdashti H (2013) Quantification of the response of germination rate to temperature in sesame (Sesamum indicum). Seed Sci Technol 41:469–473CrossRefGoogle Scholar
  8. Bakhshandeh E, Atashi S, Hafeznia M, Pirdashti H, Teixeira da Silva JA (2015) Hydrothermal time analysis of watermelon (Citrullus vulgaris cv.‘Crimson sweet’) seed germination. Acta Physiol Plant 37:1738CrossRefGoogle Scholar
  9. Bakhshandeh E, Jamali M, Afshoon E, Gholamhossieni M (2017) Using hydrothermal time concept to describe sesame (Sesamum indicum L.) seed germination response to temperature and water potential. Acta Physiol Plant 39:250CrossRefGoogle Scholar
  10. Balandary A, Rezvani Moghaddam P, Nasiri Mahalati M (2011) Determination of seed germination cardinal temperatures in short chicory (Cichorium pumilum Jacq.). Paper presented at the Second National Seed Technology Conference, MashhadGoogle Scholar
  11. Baskin CC, Baskin JM (2014) Seeds: ecology, biogeography, and, evolution of dormancy and germination. Academic Press, San Diego, p 1600Google Scholar
  12. Basto S, Dorca-Fornell C, Thompson K, Rees M (2013) Effect of pH buffer solutions on seed germination of Hypericum pulchrum, Campanula rotundifolia and Scabiosa columbaria. Seed Sci Technol 41:298–302CrossRefGoogle Scholar
  13. Bewley JD, Bradford K, Hilhorst H, Nonogaki H (2013) seeds: physiology of development, germination and dormancy, 3rd edn. Springer, New YorkCrossRefGoogle Scholar
  14. Bradford KJ (2002) Applications of hydrothermal time to quantifying and modeling seed germination and dormancy. Weed Sci 50:248–260CrossRefGoogle Scholar
  15. Chachalis D, Reddy KN (2000) Factors affecting Campsis radicans seed germination and seedling emergence. Weed Sci 48:212–216CrossRefGoogle Scholar
  16. Channaoui S, El Kahkahi R, Charafi J, Mazouz H, El Fechtali M, Nabloussi A (2017) Germination and seedling growth of a set of rapeseed (Brassica napus) varieties under drought stress conditions. Int J Agric Environ Biotechnol 2:487–494CrossRefGoogle Scholar
  17. Chauhan BS, Gill G, Preston C (2006) African mustard (Brassica tournefortii) germination in southern Australia. Weed Sci 54:891–897CrossRefGoogle Scholar
  18. Copeland LO, McDonald MF (2012) Principles of seed science and technology, 4th edn. Springer, New York, p 467Google Scholar
  19. Corbineau F, Come D (1989) Germinability and quality of Cichorium intybus L. seeds. In: VI symposium on the timing of field production of vegetables, vol. 267, pp. 183–190.  https://doi.org/10.17660/ActaHortic.1990.267.23
  20. Das S, Vasudeva N, Sharma S (2016) Cichorium intybus: a concise report on its ethnomedicinal, botanical, and phytopharmacological aspects. Drug Dev Ther 7:1–12CrossRefGoogle Scholar
  21. Derakhshan A, Bakhshandeh A, Siadat SA-A, Moradi-Telavat M-R, Andarzian SB (2018) Quantifying the germination response of spring canola (Brassica napus L.) to temperature. Ind Crops Prod 122:195–201CrossRefGoogle Scholar
  22. Dinelli G, Marotti I, Catizone P, Bosi S, Tanveer A, Abbas R, Pavlovic D (2013) Germination ecology of Ambrosia artemisiifolia L. and Ambrosia trifida L. biotypes suspected of glyphosate resistance. Open Life Sci 8:286–296Google Scholar
  23. Fakheri BA, Mousavi Nick SM, Mohammadpour Vashvaei R (2017) Effect of drought stress induced by polyethylene glycol on germination and morphological properties of fennel and ajowan. J Crop Sci Res Arid Regions 1:35–50Google Scholar
  24. Florentine A, Javaid M, Fernando N, Chauhan BS, Dowling K (2016) Influence of selected environmental factors on seed germination and seedling survival of the arid zone invasive species tobacco bush (Nicotiana glauca R. Graham). The Rangeland J 38:417–425, WesTbrooke MCrossRefGoogle Scholar
  25. Forcella F, Arnold RLB, Sanchez R, Ghersa CM (2000) Modeling seedling emergence. Field Crops Res 67:123–139CrossRefGoogle Scholar
  26. Ghaderi-Far F, Akbarpour W, Khavari F, Ehteshamnia A (2012) Determination of salinity tolerance threshold in six medicinal plants. J Plant Product 18:15–24Google Scholar
  27. Honarmand SJ, Nosratti I, Nazari K, Heidari H (2016) Factors affecting the seed germination and seedling emergence of muskweed (Myagrum perfoliatum). Weed Biol Manag 16:186–193CrossRefGoogle Scholar
  28. International Seed Testing Association (ISTA) (2009) International rules for seed testing. Seed Sci Technol:Zurich, SwitzerlandGoogle Scholar
  29. Jamil M, Lee CC, Rehman SU, Lee DB, Ashraf M, Rha ES (2005) Salinity (NaCl) tolerance of Brassica species at germination and early seedling growth. Electron J Environ Agric Food Chem 4:970–976Google Scholar
  30. Javadzadeh M, Rezvani Moghaddam P, Banayan-Aval M, Asili J (2017) Cardinal temperatures for germination of Roselle (Hibiscus sabdariffa). Iran J Seed Res 3:129–141CrossRefGoogle Scholar
  31. Javaid MM, Florentine SK, Ali HH, Chauhan BS (2018) Environmental factors affecting the germination and emergence of white horehound (Marrubium vulgare L.): a weed of arid-zone areas. Rangeland J 40:47–54CrossRefGoogle Scholar
  32. Kayacetin F, Efeoglu B, Alizadeh B (2018) Effect of NaCl and PEG-induced osmotic stress on germination and seedling growth properties in wild mustard (Sinapis arvensis L.). Anadolu 28:62–68Google Scholar
  33. Koger CH, Reddy KN, Poston DH (2004) Factors affecting seed germination, seedling emergence, and survival of texasweed (Caperonia palustris). Weed Sci 52:989–995CrossRefGoogle Scholar
  34. Mamedi A, Tavakkol Afshari R, Oveisi M (2017) Cardinal temperatures for seed germination of three Quinoa (Chenopodium quinoa Willd.) cultivars. Iran J Field Crops Sci 48:89–100Google Scholar
  35. Mesgaran MB, Onofri A, Mashhadi HR, Cousens RD (2017) Water availability shifts the optimal temperatures for seed germination: a modelling approach. Ecol Model 351:87–95CrossRefGoogle Scholar
  36. Michel BE, Radcliffe D (1995) A computer program relating solute potential to solution composition for five solutes. Agron J 87:126–130CrossRefGoogle Scholar
  37. Mobli A, Ghanbari A, Rastgoo M (2018) Determination of cardinal temperatures of flax-leaf alyssum (Alyssum linifolium Steph. ex. Willd.) response to salinity, pH and drought stress. Weed Sci 66:470–476CrossRefGoogle Scholar
  38. Nandagopal S, Kumari BR (2007) Phytochemical and antibacterial studies of Chicory (Cichorium intybus L.)—a multipurpose medicinal plant. Adv Biol Res 1:17–21Google Scholar
  39. Neumann P (1997) Salinity resistance and plant growth revisited. Plant Cell Environ 20:1193–1198CrossRefGoogle Scholar
  40. Nosratti I, Soltanabadi S, Honarmand SJ, Chauhan BS (2017) Environmental factors affect seed germination and seedling emergence of invasive Centaurea balsamita. Crop Pasture Sci 68:583–589CrossRefGoogle Scholar
  41. Okçu G, Kaya MD, Atak M (2005) Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). Turk J Agric For 29:237–242Google Scholar
  42. Parihar P, Singh S, Singh R, Singh VP, Prasad SM (2015) Effect of salinity stress on plants and its tolerance strategies: a review. Environ Sci Pollut Res 22:4056–4075CrossRefGoogle Scholar
  43. Parmoon G, Moosavi SA, Siadat SA (2018) How salinity stress influences the thermal time requirements of seed germination in Silybum marianum and Calendula officinalis. Acta Physiol Plant 40:175CrossRefGoogle Scholar
  44. SAS Institute Inc (2013) SAS/STAT user’s guide. SAS Institute Inc., CaryGoogle Scholar
  45. Seal CE, Barwell LJ, Flowers TJ, Wade EM, Pritchard HW (2018) Seed germination niche of the halophyte Suaeda maritima to combined salinity and temperature is characterised by a halothermal time model. Environ Exp Bot 155:177–184CrossRefGoogle Scholar
  46. Seghatoleslami MJ (2010) Effect of salinity on germination of three medicinal species of Satureja (Satureja hortensis L.), Chicory (Cichorium intybus L.) and Artichoke (Cynara scolymus L.). Iran J Field Crops Res 8:818–823Google Scholar
  47. Street RA, Sidana J, Prinsloo G (2013) Cichorium intybus: traditional uses, phytochemistry, pharmacology, and toxicology. Evid Based Complement Altern Med 2013Google Scholar
  48. Toosi AF, Bakar BB, Azizi M (2014) Effect of drought stress by using PEG 6000 on germination and early seedling growth of Brassica juncea var. Ensabi Scientific Papers Series A Agron 57:360–363Google Scholar
  49. Xu H, Su W, Zhang D, Sun L, Wang H, Xue F, Zhai S, Zou Z, Wu R (2017) Influence of environmental factors on Cucumis melo L. var. agrestis Naud. seed germination and seedling emergence. PloS One 12:e0178638CrossRefGoogle Scholar
  50. Yin X, Kropff MJ, McLaren G, Visperas RM (1995) A nonlinear model for crop development as a function of temperature. Agric For Meteorol 77:1–16CrossRefGoogle Scholar
  51. Zarghani H, Mijani S, Nasrabadi SE (2014) Temperature effects on the seed germination of some perennial and annual species of Asteraceae family. Plant Breeding Seed Sci 69:3–14CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  1. 1.Sari Agricultural Sciences and Natural Resources UniversitySariIran
  2. 2.Genetics and Agricultural Biotechnology Institute of Tabarestan and Sari Agricultural Sciences and Natural Resources UniversitySariIran

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