Algerian maize populations from the Sahara desert as potential sources of drought tolerance

  • Abderahmane Djemel
  • Lorena Álvarez-Iglesias
  • Rogelio Santiago
  • Rosa Ana Malvar
  • Nuria Pedrol
  • Pedro RevillaEmail author
Original Article


Drought affects maize (Zea mays L.) performance from seedling to grain filling. Sources of drought tolerance at multi-scale growth are crucial for maize breeders. The objectives of this work were to identify new sources and mechanisms of drought tolerance and to study the traits controlling plant growth under drought and their associations with yield. We evaluated a collection of 18 maize populations from the Algerian Saharan oases under simulated drought conditions using Polyethylene glycol 6000, and in the field. The genotype × treatment interactions were more significant under field than under control conditions. Under field conditions, based on general agronomic performance, PI527476, PI542678 and PI527474 were the most drought-tolerant populations. PI527476 was the most tolerant population for germination-related traits, PI542678 exhibited high final germination, high number of secondary roots and positive water use efficiency (WUE), while PI527474 presented high number of secondary roots number and WUE. PI542685 was drought tolerant at seedling stage, characterized by high germination, long roots, heavy root, high number of secondary roots, and stable shoot/root weight ratio. Germination, shoot/root weight, root dry weight at seedling stage, and dry weight of secondary roots were the most significant seedling growth-related traits affecting WUE and yield under drought. Differences were detected among populations under stress conditions at multi-scale growth phases with some common genetic dependency of yield on seedling-related traits. Therefore, these Saharan populations could provide favorable alleles for drought tolerance for breeding programs, and the use of seedling-related traits, especially root performance, as secondary yield-related traits could lead to increased gain yield selection under drought conditions.


Zea mays L. Drought stress Seedling growth Algerian landraces 



Research was supported by the Spanish Plan for Research and Development (Project codes AGL2013-48852-C3-1-R, AGL2016-77628-R and FEDER) and the École Nationale Supérieure Agronomique. Seed from Algerian populations was provided by the North Central Regional Plant Introduction Station of the USA. The Instituto de Ordenación Rural de Ourense (INORDE) has hosted one trial.

Supplementary material

11738_2019_2806_MOESM1_ESM.doc (191 kb)
Supplementary material 1 (DOC 191 KB)


  1. Abdel-Ghani AH, Neumann K, Wabila C, Sharma R, Dhanagond S, Owais SJ et al (2015) Diversity of germination and seedling traits in a spring barley (Hordeum vulgare L.) collection under drought simulated conditions. Genet Res Crop Evol 62:275–292CrossRefGoogle Scholar
  2. Aci MM, Revilla P, Morsli A, Djemel A, Belalia N, Kadri Y et al (2013) Genetic diversity in Algerian maize (Zea mays L.) landraces using SSR markers. Maydica 58:304–310Google Scholar
  3. Akinwale RO, Awosanmi FE, Ogunniyi OO, Fadoju AO (2017) Determinants of drought tolerance at seedling stage in early and extra-early maize hybrids. Maydica 62:1Google Scholar
  4. Almeida GD, Nair S, Borem A, Cairns J, Trachsel S, Ribaut JM et al 2014Molecular mapping across three populations reveals a QTL hotspot region on chromosome 3 for secondary traits associated with drought tolerance in tropical maize. Mol Breed 34: 701–715Google Scholar
  5. Álvarez-Iglesias L, de la Roza-Delgado B, Reigosa MJ, Revilla P, Pedrol N (2017) A simple, fast and accurate screening method to estimate maize (Zea mays L.) tolerance to drought at early stages. Maydica 62:12Google Scholar
  6. Álvarez-Iglesias L, Djemel A, Malvar RA, Gutièrrez J, Reyes R, Pedrol N, Revilla P (2018) Variability and mechanisms of drought tolerance in maize populations from Honduras. Maydica 63(2):M20Google Scholar
  7. Araus JL, Serret MD, Edmeades GO (2012) Phenotyping maize for adaptation to drought. Front Physiol 3:1–20CrossRefGoogle Scholar
  8. Aslam M, Maqbool MA, Cengiz R (2015) Drought stress in maize (Zea mays L.). Effects, resistance mechanisms, global achievements and biological strategies for improvement. Springer Briefs in Agriculture, London (Springer Cham)CrossRefGoogle Scholar
  9. Bänziger M, Araus JL (2007) Recent advances in breeding maize for drought and salinity stress tolerance. In: Jenks MA, Hasegawa PM, Jain SM (eds) Advances in molecular breeding toward drought and salt tolerant crops. Springer, The Netherlands, pp 587–601CrossRefGoogle Scholar
  10. Berger B, Parent B, Tester M (2010) High-throughput shoot imaging to study drought responses. J Exp Bot 61:3519–3528PubMedCrossRefGoogle Scholar
  11. Betrán FJ, Beck D, Banziger M, Edmeades GO (2003) Genetic analysis of inbred and hybrid grain yield under stress and nonstress environments in tropical maize. Crop Sci 43:807–817CrossRefGoogle Scholar
  12. Bhargava S, Sawant S (2013) Drought stress adaptation: metabolic adjustment and regulation of gene expression. Plant Breed 132:21–32CrossRefGoogle Scholar
  13. Bolaños J, Edmeades GO (1996) The importance of the anthesis-silking interval in breeding for drought tolerance in tropical maize. Field Crops Res 48:65–80CrossRefGoogle Scholar
  14. Campos H, Cooper M, Habben JE, Edmeades GO, Schussler JR (2004) Improving drought tolerance in maize: a view from industry. Field Crop Res 90:19–34CrossRefGoogle Scholar
  15. Chen JP, Xu WW, Velten J, Xin ZG, Stout J (2012) Characterization of maize inbred lines for drought and heat tolerance. J Soil Water Conserv 67:354–364CrossRefGoogle Scholar
  16. Djemel A, Revilla P, Hanifi-Mekliche L, Malvar RA, Álvarez A, Khelifi L (2012) Maize (Zea mays L.) from the Saharan oasis: adaptation to temperate areas and agronomic performance. Genet Res Crop Evol 59:1493–1504CrossRefGoogle Scholar
  17. Djemel A, Cherchali FZ, Benchikh Le-Hocine M, Malvar RA, Revilla P (2018) Assessment of drought tolerance among Algerian maize populations from oases of the Saharan. Euphytica 214(8):149CrossRefGoogle Scholar
  18. Emerson R, Hoover A, Ray A, Lacey J, Cortez M, Payne C et al (2014) Drought effects on composition and yield for corn stover, mixed grasses, and miscanthus as bioenergy feedstocks. Biofuels 5: 275–291CrossRefGoogle Scholar
  19. Ertiro BT, Beyene Y, Das B, Mugo S, Olsen M, Oikeh S et al (2017) Combining ability and testcross performance of drought-tolerant maize inbred lines under stress and non-stress environments in Kenya. Plant Breed 136:197–205PubMedPubMedCentralCrossRefGoogle Scholar
  20. Fisher M, Tsedeke Abate Y, Lunduka RW, Asnake W, Alemayehu Y, Madulu RB (2015) Drought tolerant maize for farmer adaptation to drought in sub-Saharan Africa: determinants of adoption in eastern and southern Africa. Clim Change 133:283–299CrossRefGoogle Scholar
  21. Flint-Garcia SA, Thuillet AC, Yu J, Pressoir G, Romero SM, Mitchell SE et al (2005) Maize association population: a high-resolution platform for quantitative trait locus dissection. Plant J 44:1054–1064PubMedCrossRefGoogle Scholar
  22. Grzesiak MT, Waligorski P, Janowiak F, Marcinska I, Hura K, Szczyrek P, Głab T (2013) The relations between drought susceptibility index based on grain yield (DSI GY) and key physiological seedling traits in maize and triticale genotypes. Acta Physiol Plant 35:549–563CrossRefGoogle Scholar
  23. Hallauer AR, Carena MJ, Filho JBM (2010) Quantitative genetics in maize breeding. Handb Plant Breed. CrossRefGoogle Scholar
  24. Haq AU, Tahir MHN, Ahsan M, Ahmad R, Akram HM (2015) Screening and inheritance pattern studies of maize seedlings under normal and water stress conditions. Pak J Life Soc Sci 13:97–103Google Scholar
  25. Harrison M, Tardieu F, Dong Z, Messina CD, Hammer GL (2014) Characterizing drought stress and trait influence on maize yield under current and future conditions. Glob Chang Biol 20:867–878PubMedCrossRefGoogle Scholar
  26. Hawtin G, Iwanaga M, Hodgkin T (1996) Genetic resources in breeding for adaptation. Euphytica 92:255–266CrossRefGoogle Scholar
  27. Hoisington D, Khairallah M, Reeves T, Ribaut JM, Skovmand B, Taba S et al (1999) Plant genetic resources: What can they contribute toward increased crop productivity? Proc Natl Acad Sci USA 96:5937–5943PubMedCrossRefGoogle Scholar
  28. James C (2002) Global review of commercialized transgenic crops: feature: Bt Maize. ISAAA Briefs No. 29. ISAAA, Ithaca, 2003Google Scholar
  29. Khan NH, Ahsan M, Naveed M, Sadaqat HA, Javed I (2016) Genetics of drought tolerance at seedling and maturity stages in Zea mays L. Span J Agric Res 14(3):e0705CrossRefGoogle Scholar
  30. Liu M, Li M, Liu K, Sui N (2015) Effects of drought stress on seed germination and seedling growth of different maize varieties. J Agric Sci 7:231–240Google Scholar
  31. Messina CD, Sinclair TR, Hammer GL, Curan D, Thompson J, Oler Z, Gho C, Cooper M (2015) Limited transpiration trait may increase maize drought tolerance in the US Corn Belt. Agron J 107:1978–1986CrossRefGoogle Scholar
  32. Messmer R, Fracheboud Y, Bänziger M, Vargas M, Stamp P, Ribaut JM (2009) Drought stress and tropical maize: QTL-by-environment interactions and stability of QTLs across environments for yield components and secondary traits. Theor Appl Genet 119:913–930PubMedCrossRefGoogle Scholar
  33. Mikić S, Zorić M, Stanisavljević D, Kondić-Špika A, Brbaklić L, Kobiljski B et al (2016) Agronomic and molecular evaluation of maize inbred lines for drought tolerance. Span J Agric Res 14:e0711. CrossRefGoogle Scholar
  34. Pandey RK, Maranville JW, Chetima MM (2006) Deficit irrigation and nitrogen effects on maize in a Sahelian environment: II. Shoot growth, nitrogen uptake and water extraction. Agric Water Manag 2000 46:15–27CrossRefGoogle Scholar
  35. Rosegrant MW, Msangi S, Ringler C, Sulser TB, Zhu T, Cline SA (2008) Model for policy analysis of agricultural commodities and trade (IMPACT): model description. International Food Policy Research Institute, Washington, DCGoogle Scholar
  36. Ruta N, Stamp P, Liedgens M, Fracheboud Y, Hund A (2010) Collocations of QTLs for seedling traits and yield components of tropical maize under water stress conditions. Crop Sci 50:1385–1392CrossRefGoogle Scholar
  37. SAS Institute Inc (2008) SAS/STAT® 9.2 user’s guide. SAS Institute Inc., CaryGoogle Scholar
  38. Tardieu F (2013) Plant response to environmental conditions: assessing potential production, water demand, and negative effects of water deficit. Front Phys 18:4–17Google Scholar
  39. Wang X, Wang H, Liu S, Ferjani A, Li J, Yan J et al (2016) Genetic variation in ZmVPP1 contributes to drought tolerance in maize seedlings. Nat Genet 48:1233–1241PubMedCrossRefGoogle Scholar
  40. Welcker C, Boussuge B, Bencivenni C, Ribaut JM, Tardieu F (2007) Are source and sink strengths genetically linked in maize plants subjected to water deficit? A QTL study of the responses of leaf growth and of anthesis-silking interval to water deficit. J Exp Bot 58:339–349PubMedCrossRefGoogle Scholar
  41. Witt S, Galicia L, Lisec J, Cairns J, Tiessen A, Araus JL et al (2012) Metabolic and phenotypic responses of greenhouse-grown maize hybrids to experimentally controlled drought stress. Mol Plant 5:401–417PubMedCrossRefGoogle Scholar
  42. Zhao F, Zhang D, Zhao Y, Wang W, Yang H, Tai F et al (2016) The difference of physiological and proteomic changes in maize leaves adaptation to drought, heat, and combined both stresses. Front Plant Sci 7:1471. PubMedPubMedCentralCrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  • Abderahmane Djemel
    • 1
    • 2
    • 4
  • Lorena Álvarez-Iglesias
    • 2
    • 3
  • Rogelio Santiago
    • 2
    • 4
  • Rosa Ana Malvar
    • 2
    • 3
  • Nuria Pedrol
    • 2
    • 4
  • Pedro Revilla
    • 2
    • 3
    Email author
  1. 1.École Nationale Supérieure AgronomiqueAlgerAlgeria
  2. 2.Unidad Asociada Agrobiología Ambiental, Calidad de Suelos y PlantasUniversidad de Vigo and Misión Biológica de Galicia (CSIC)PontevedraSpain
  3. 3.Misión Biológica de Galicia (CSIC)PontevedraSpain
  4. 4.Department of Plant Biology and Soil ScienceUniversity of VigoVigoSpain

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