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Chloroplast ultrastructure and hormone endogenous levels are differently affected under light and dark conditions during in vitro culture of Guadua chacoensis (Rojas) Londoño & P. M. Peterson

  • Luiza Giacomolli Polesi
  • Hugo Pacheco de Freitas Fraga
  • Leila do Nascimento Vieira
  • Angelo Schuabb Heringer
  • Thiago Sanches Ornellas
  • Henrique Pessoa dos Santos
  • Miguel Pedro Guerra
  • Rosete PescadorEmail author
Original Article
  • 128 Downloads

Abstract

Guadua chacoensis (Poaceae) is a woody bamboo native from the Atlantic forest biome. Morphogenetic and physiological studies are scarce in bamboos, and tissue culture-based biotechnologies tools can be used to investigate ultrastructure and physiological processes as well as to mass-propagate specific genotypes. This study evaluated the effect of light and dark conditions on chloroplast biogenesis as well as in the endogenous levels of zeatin (Z), abscisic acid (ABA), gibberellic acid (GA4), and jasmonic acid (JA) during in vitro culture of G. chacoensis. An increase was observed, followed by a decrease in starch content in response to light treatment, and in contrast, in darkness, an accumulation of starch which is associated to amyloplast formation at day 30 was observed. No etioplast formation was observed even in the dark and this was associated with the presence of fully developed chloroplast at the beginning of the experiment. Z levels quantified showed distinct behavior, as in light, no difference in the levels was observed, except at day 10, and in darkness, the levels increased along the evaluation time. ABA, GA4, and JA biosynthesis increased along the time of evaluation in light condition. In contrast, in darkness, the levels remained unaltered, excepted for an increase in JA levels at day 10, suggesting a strong relationship between light and these phytohormone levels. Possible reasons why light’s presence and absence cause changes in chloroplast ultrastructure and hormone endogenous levels in in vitro cultivated culms of G. chacoensis are discussed.

Keywords

Bamboos Micropropagation Transmission electron microscopy Plant physiology Phytohormone 

Notes

Acknowledgements

This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 457726/2013-0, and 306126/2013-3). LGP thank to CAPES for the master’s degree scholarship. The authors also thank to Central Laboratory of Electron Microscopy (LCME) of the Federal University of Santa Catarina, Brazil.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2019

Authors and Affiliations

  • Luiza Giacomolli Polesi
    • 1
  • Hugo Pacheco de Freitas Fraga
    • 2
  • Leila do Nascimento Vieira
    • 1
  • Angelo Schuabb Heringer
    • 1
  • Thiago Sanches Ornellas
    • 1
  • Henrique Pessoa dos Santos
    • 3
  • Miguel Pedro Guerra
    • 1
    • 4
  • Rosete Pescador
    • 1
    Email author
  1. 1.Graduate Program in Plant Genetic Resources, Laboratory of Plant Developmental Physiology and GeneticsFederal University of Santa CatarinaFlorianópolisBrazil
  2. 2.Graduate Program in Botany, Laboratory of Plant MicropropagationFederal University of ParanáCuritibaBrazil
  3. 3.Brazilian Agricultural Research Corporation (Embrapa), Laboratory of Plant PhysiologyEmbrapa Grape and WineBento GonçalvesBrazil
  4. 4.Tree and Timber InstituteNational Research Council of ItalyFlorenceItaly

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