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Acta Physiologiae Plantarum

, 40:184 | Cite as

Evaluation of genetic diversity and population structure of polygonati rhizoma germplasms: implications for better crop development and conservation of a traditional Chinese medicine

  • Jie Jiao
  • Zhenqing Bai
  • Wenli Huang
  • Pengguo Xia
  • Feng Liu
  • Cunde Ma
  • Zongsuo Liang
Original Article
  • 77 Downloads

Abstract

Polygonati rhizoma (PR) is an important and widely used product in Chinese traditional medicine and edible goods. The time-consuming nature of breaking dormancy in both rhizomes and seeds means that improving variety selection is limited to collection, identification, and selection of germplasms. In this study, we used two DNA-based molecular marker techniques—inter simple sequence repeat (ISSR) and start codon targeted (SCoT)—to assess the genetic diversity and population structure among PR source plants collected from 47 different regions and belonging to 12 populations (P1–P12). For molecular markers analysis, 15 ISSR and 10 SCoT markers were tested. Total number of 159 fragments (150–4000 bp) were amplified based on ISSR analysis with a range from 6 to 17 bands, 153 of them were polymorphic, ranging from 97.27 to 100%. For SCoT analysis, 164 polymorphic bands (150–5000 bp) were observed, varying from 14 to 19 bands for each primer. Nei’s genetic diversity analysis showed that the highest value was found in P11 for ISSR and P4 for SCoT markers. The highest Nei’s genetic diversity value was observed in P5 for combined markers and the low in P2. Nei’s dendrogram constructed with combined markers indicated a 75–89% of genetic similarity coefficient among populations. Population structure analysis revealed an optimum number of three groups, the same as their geographical distribution. This knowledge on PR genetic diversity can be used in future breeding programs, genetic improvement, product enhancement, and germplasms conservation.

Keywords

Polygonati rhizoma Genetic diversity Population structure Molecular marker 

Notes

Acknowledgements

This work was supported by the Huimin Plan of Ministry of Science and Technology [grant numbers 2012GS610102] and the Support Plan Project of the state Ministry of Science &Technology [grant number 2015BAC01B03].

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11738_2018_2756_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 KB)
11738_2018_2756_MOESM2_ESM.tif (181 kb)
Supplementary material 2 (TIF 180 KB)
11738_2018_2756_MOESM3_ESM.tif (397 kb)
Supplementary material 3 (TIF 397 KB)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  1. 1.College of Life ScienceNorthwest A&F UniversityYanglingChina
  2. 2.College of Life ScienceYan’an UniversityYan’anChina
  3. 3.College of PharmacyShaanxi University of Chinese MedicineXianyangChina
  4. 4.College of Life ScienceZhejiang SCI-TECH UniversityHangzhouChina
  5. 5.Shaanxi Institute of International Trade & CommerceXianyangChina
  6. 6.Research DepartmentShaanxi Buchang Pharmaceutical Co., Ltd.Xi’anChina

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