Acta Physiologiae Plantarum

, 40:165 | Cite as

Effects of lovastin, fosmidomycin and methyl jasmonate on andrographolide biosynthesis in the Andrographis paniculata

  • Rakesh Kumar SinhaEmail author
  • Shiv Narayan Sharma
  • Shiv S. Verma
  • Jenu Zha
Original article


Andrographolide is a diterpene secondary metabolite product of Andrographis paniculata. It has been known to be a pharmaceutically important compound synthesized via the cytosolic mevalonate (MVA) and the plastidial 2-C-methyl-d-erythritol-4-phosphate (MEP) pathways. To understand the biosynthetic pathway of andrographolide biosynthesis in Andrographis paniculata, lovastatin, fosmidomycin and methyl jasmonate (MeJA) were used to inhibit the key enzymes 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), and 1-deoxy-d-xylulose-5-phosphate reducto-isomerase (DXR) involved in the synthesis of andrographolide in the MVA and MEP pathways, respectively. The inhibition of andrographolide accumulation was linked with the expression level of the studied regulatory genes, 3-hydroxy-3-methyl glutaryl coenzyme A synthase (hmgs), 3-hydroxy-3-methyl glutaryl coenzyme A reductase (hmgr), 1-deoxyxylulose-5-phosphate synthase (dxs), 1-deoxyxylulose-5-phosphate reductoisomerase (dxr), 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase (hds),1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (hdr), 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase(isph), isopentenyl diphosphate isomerase (ipp), geranylgeranyl diphosphatesynthase (ggps) of the MVA and MEP pathways. The pathways associated transcript expression level, and andrographolide biosynthesis was significantly modulated by the inhibitors indicating that the andrographolide biosynthesis is strongly responsive at the transcriptional level. The results demonstrated that both pathways can contribute to the biosynthesis of andrographolide in A. paniculata. Both hmgr and dxr played a critical role consistent with some crossover between MVA and MEP pathways in andrographolide biosynthesis.


Andrographis paniculata Andrographolide Mevalonate Fosmidomycin Lovastatin Methyl jasmonate 



Dimethylallyl diphosphate




1-Deoxyxylulose-5-phosphate reductoisomerase


1-Deoxyxylulose-5-phosphate synthase




Geranyl geranyl diphosphate


Geranylgeranyl diphosphate synthase


1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase


1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase


(E)-4-hydroxy-3-methylbut-2-enyl pyrophosphate


3-Hydroxy-3-methyl glutaryl coenzyme A reductase


3-Hydroxy-3-methyl glutaryl coenzyme A synthase


Isopentenyl diphosphate


1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase




Mevalonic acid



Financial support from Indira Gandhi Krishi Vishwavidyalaya (IGKV), Raipur is gratefully acknowledged. Thanks to Dr. D.K. Sharma, Former Head, Department of Plant Molecular Biology and Biotechnology for arranging funds and facilities. We thank Jordan Pepper for providing an English proof read.


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  • Rakesh Kumar Sinha
    • 1
    • 2
    Email author
  • Shiv Narayan Sharma
    • 3
  • Shiv S. Verma
    • 4
  • Jenu Zha
    • 3
  1. 1.Faculty of Biology and Environmental ProtectionUniversity of Silesia in KatowiceKatowicePoland
  2. 2.Department of Biophysics and Biochemistry of PlantsInstitute of Plant Molecular Biology, Biology Centre of the ASCRČeské BudějoviceCzechia
  3. 3.Department of Genetics and Plant BreedingIGKVRaipurIndia
  4. 4.Agricultural and Agri-Food CanadaLethbridgeCanada

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