Optimization of culturing conditions for production of somatic embryos and lignins of Schisandra chinensis (Turcz.) Baill
- 26 Downloads
Schisandra chinensis (Turcz.) Baill. is a valuable medicinal plant species increasingly used in phytotherapy worldwide. This study systematically detected the lignin content and production during somatic embryogenesis of S. chinensis. The effect of various culture parameters on biomass accumulation and lignin production were also examined to optimize the accumulation of lignins in SEs in bioreactors, including the culture method, inoculum density, aeration volume and photoperiod. An inoculum density of 20 g L− 1 embryogenic calli enhanced production of lignin, while 30 g L− 1 embryogenic calli increased the biomass of somatic embryos. During somatic embryo induction, an aeration volume of 0.2 vvm and photoperiod of 16 h day− 1 were found to be optimal for biomass accumulation and lignin production. An approximately threefold increase in the biomass production rate and a fourfold increase in the total lignin production rate in SEs were achieved in bioreactors than on solid medium. The present study indicated, therefore, that the culturing of S. chinensis somatic embryos in bioreactors is an effective method for the industrialized production of lignin in vitro.
KeywordsSchisandra chinensis (Turcz.) Baill. Bioreactor culture Inoculum density Aeration volume Photoperiod Lignin
Murashige and Skoog
- 2, 4-D
2, 4-Dichlorophenoxyacetic acid
This work was supported by the Project of Science and Technology Department of Jilin Province [No. 20150204064YY] and the Project of Science and Technology Department of Jilin Province [No. 20160209004YY].
- Jwa CS, Yang YT, Koh JS (2000) Changes in free sugars, organic acids, free amino acids and minerals by harvest time and parts of Acanthopanax koreanum. J Korean Soc Agric Chem Biotechnol 43:106–109Google Scholar
- Lee EJ, Moh SH, Paek KY (2011) Influence of inoculum density and aeration volume on biomass and bioactive compound production in bulb-type bubble bioreactor cultures of Eleutherococcus koreanum Nakai. Biores Technol 102:7165–7170. https://doi.org/10.1016/j.biortech.2011.04.076 CrossRefGoogle Scholar
- Mišić D, Šiler B, Marijana S, Djurickovic MS, Živković JN, Jovanović V, Giba Z (2013) Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor. Process Biochem 48:1587–1591. https://doi.org/10.1016/j.procbio.2013.07.015 CrossRefGoogle Scholar
- Mocan A, Schafberg M, Crișan G, Rohn S (2016) Determination of lignins and phenolic components of Schisandra chinensis (Turcz.) Baill. using HPLC-ESI-ToF-MS and HPLC-online TEAC: contribution of individual components to overall antioxidant activity and comparison with traditional antioxidant assays. J Funct Foods 24:579–594. https://doi.org/10.1016/j.jff.2016.05.007 CrossRefGoogle Scholar
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
- Rajesh M, Sivanandhan G, Subramanyam K, Kapildev G, Jaganath B, Kasthurirengan S, Manickavasagam M, Ganapathi A (2014) Establishment of somatic embryogenesis and podophyllotoxin production in liquid shake cultures of Podophyllum hexandrum Royle. Ind Crops Prod 60:66–74. https://doi.org/10.1016/j.indcrop.2014.05.046 CrossRefGoogle Scholar
- Sun D, Li Q, Li HB, Ai J, Qin HY, Piao ZY (2015) Plantlet regeneration through somatic embryogenesis in Schisandra chinensis (Turcz.) Baill. and analysis of genetic stability of regenerated plants by SRAP markers. Bangladesh J Bot 44:881–888Google Scholar
- Szopa A, Ekiert R, Ekiert H (2017) Current knowledge of Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) as a medicinal plant species: a review on the bioactive components, pharmacological properties, analytical and biotechnological studies. Phytochem Rev 16(2):195–218. https://doi.org/10.1007/s11101-016-9470-4 CrossRefPubMedGoogle Scholar