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Acta Physiologiae Plantarum

, 40:160 | Cite as

The modulation of various physiochemical changes in Bruguiera cylindrica (L.) Blume affected by high concentrations of NaCl

  • Sruthi Palliyath
  • Jos T. Puthur
Original Article
  • 76 Downloads

Abstract

Bruguiera cylindrica is a major mangrove species in the tropical mangrove ecosystems and it grows in a wide range of salinities without any special features for the excretion of excess salt. Therefore, the adaptation of this mangrove to salinity could be at the physiological and biochemical level. The 3-month-old healthy plantlets of B. cylindrica, raised from propagules were treated with 0 mM, 400 mM, 500 mM and 600 mM NaCl for 20 days under hydroponic culture conditions provided with full strength Hoagland medium. The modulation of various physiochemical changes in B. cylindrica, such as chlorophyll a fluorescence, total chlorophyll content, dry weight, fresh weight and water content, Na+ accumulation, oxidation and antioxidation (enzymatic and non-enzymatic) features were studied. Total chlorophyll content showed very minute decrease at 500 mM and 600 mM NaCl treatment for 20 days and the water content percentage was decreased both in leaf and root tissues with increasing concentration. A significant increase of Na+ content of plants from 84.505 mM/plant dry weight in the absence of NaCl to 543.38 mM/plant dry weight in plants treated with 600 mM NaCl was recorded. The malondialdehyde and the metabolites content associated with stress tolerance (amino acid, total phenols and proline) showed an increasing pattern with increasing NaCl concentration as compared to the control in both leaf and root tissues but the increase recorded in plantlets subjected to 500 mM was much less, indicating the tolerance potential of this species towards 500 mM NaCl. The significant decrease of sugar content was found only in 600 mM NaCl on 20 days of treatment, showing that the process of sugar synthesis was negatively affected but the same process remains less affected at 500 mM NaCl. A slight reduction in ascorbate and glutathione content and very less increase in carotenoid content were observed at 500 mM and 600 mM NaCl stress. Antioxidant enzymes (APX, GPX, SOD and CAT) showed an enhanced activity in all the treatments and the increased activity was more significant in 600 mM treated plants. The result establishes that B. cylindrica tolerates high NaCl concentration, to the extent of 500 mM NaCl without any major inhibition on photosynthesis and metabolite accumulation. Understanding the modulation of various physiological and biochemical changes of B. cylindrica at high levels of NaCl will help us to know the physiochemical basis of tolerance strategy of this species towards high NaCl.

Keywords

Bruguiera cylindrica Mangroves Metabolic regulation Chlorophyll a fluorescence NaCl stress 

Notes

Acknowledgements

Author greatly acknowledges the financial assistance provided by Department of Science and Technology, New Delhi; through INSPIRE Programme (IF131045) for the research work and corresponding author express sincere thanks to KSCSTE for funding through research grant (KSCSTE/5179/2017-SRSLS) also Dr. K. V. Ajayan, Young scientist Fellow, DST-SERB, New Delhi for language correction and proof reading.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  1. 1.Plant Physiology and Biochemistry Division, Department of BotanyUniversity of CalicutMalappuramIndia

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