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Cold plasma relieved toxicity signs of nano zinc oxide in Capsicum annuum cayenne via modifying growth, differentiation, and physiology

  • Alireza Iranbakhsh
  • Zahra Oraghi Ardebili
  • Narges Oraghi Ardebili
  • Mahmood Ghoranneviss
  • Nasrin Safari
Original Article

Abstract

Taking functional scientific devices and metal-based nanoparticles into account, the present research was carried out to evaluate the plant (Capsicum annuum) responses to cold plasma and zinc oxide nanoparticle (nZnO) in in vitro and pot conditions. Seeds were exposed to plasma (0.84 W/cm2 surface power densities) with three exposure times (0, 60, and 120 s) and/or two concentrations of nZnO (0 and 100 mgl− 1). The treated seeds were cultured in hormone-free MS medium (MS) or supplemented with 2 mgl− 1 BA and 0.5 mgl− 1 IAA (MSH). The seed pre-treatment with plasma enhanced a germination process and plant early growth, in contrast with the nZnO treatment. The treatment of nZnO significantly decreased the total fresh mass and leaf area in the seedlings grown in both culture media, while its growth-delaying impact was mitigated by the plasma treatment. The chlorophyll a and carotenoid were increased to 39.35 and 32% for the plasma-treated seedlings, respectively, than the control. The plasma and/or nZnO treatments acted as effective elicitors to induce the peroxidase activities in both culture media. Similarly, the activities of phenylalanine ammonia-lyase and soluble phenols were found to be significantly higher in the plasma and/or nZnO groups in both roots and leaves. Interestingly, inhibiting effects of nZnO on xylem differentiation was alleviated by the plasma treatments. In the pot condition, soaking seeds before the plasma treatment was the most effective method to affect plant growth. This is a first report reflecting the potential benefits of the cold plasma treatment to improve plant growth and resistance to the nanoparticle.

Keywords

Applied physics Nanoparticle Nitric oxide Non-thermal plasma Plant growth Seed priming Stress Zinc oxide 

Notes

Acknowledgements

The authors would like to thank MSc. Hamed Nikmaram, MSc. Maryam Amini, and MSc. Gasem Asgari for their benevolent and professional collaborations in the research procedure. Corresponding author specially would like to acknowledge of Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Supplementary material

11738_2018_2730_MOESM1_ESM.docx (1.9 mb)
Supplementary material 1 (DOCX 1968 KB)

References

  1. Asgari-Targhi G, Iranbakhsh A, Ardebili ZO (2018) Potential benefits and phytotoxicity of bulk and nano-chitosan on the growth, morphogenesis, physiology, and micropropagation of Capsicum annuum. Plant Physiol Biochem 127:393–402CrossRefPubMedGoogle Scholar
  2. Baytak AK, Aslanoglu M (2017) Sensitive determination of capsaicin in pepper samples using a voltammetric platform based on carbon nanotubes and ruthenium nanoparticles. Food Chem 228:152–157CrossRefPubMedGoogle Scholar
  3. Beaudoin-Eagan LD, Thorpe TA (1985) Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiol 78:438–441CrossRefPubMedPubMedCentralGoogle Scholar
  4. Boonyanitipong P, Kositsup B, Kumar P, Baruah S, Dutta J (2011) Toxicity of ZnO and TiO2 nanoparticles on germinating rice seed Oryza sativa L. Int J Biosci Biochem Bioinform 1:282Google Scholar
  5. Bußler S, Herppich WB, Neugart S, Schreiner M, Ehlbeck J, Rohn S, Schlüter O (2015) Impact of cold atmospheric pressure plasma on physiology and flavonol glycoside profile of peas (Pisum sativum ‘Salamanca’). Food Res Int 76:132–141CrossRefGoogle Scholar
  6. Buzea C, Pacheco II, Robbie K (2007) Nanomaterials and nanoparticles: sources and toxicity. Biointerphases 2:MR17–MR71CrossRefPubMedGoogle Scholar
  7. Chen HH, Chen YK, Chang HC (2012) Evaluation of physicochemical properties of plasma treated brown rice. Food Chem 135:74–79CrossRefGoogle Scholar
  8. Chen J, Liu X, Wang C, Yin S-S, Li XL, Hu WJ, Simon M, Shen ZJ, Xiao Q, Chu CC (2015) Nitric oxide ameliorates zinc oxide nanoparticles-induced phytotoxicity in rice seedlings. J Hazard Mat 297:173–182CrossRefGoogle Scholar
  9. Chithra MJ, Sathya M, Pushpanathan K (2015) Effect of pH on crystal size and photoluminescence property of ZnO nanoparticles prepared by chemical precipitation method. Acta Metall Sin 28:394–404CrossRefGoogle Scholar
  10. Fernández-Marcos M, Sanz L, Lewis DR, Muday GK, Lorenzo O (2011) Nitric oxide causes root apical meristem defects and growth inhibition while reducing PIN-FORMED 1 (PIN1)-dependent acropetal auxin transport. Proceed Nat Acad Sci 108:18506–18511CrossRefGoogle Scholar
  11. Filatova II, Azharonok VV, Goncharik SV, Lushkevich VA, Zhukovsky AG, Gadzhieva GI (2014) Effect of rf plasma treatment on the germination and phytosanitary state of seeds. J Appl Spectrosc 81(2):250–256CrossRefGoogle Scholar
  12. Fry SC, Aldington S, Hetherington PR, Aitken J (1993) Oligosaccharides as signals and substrates in the plant cell wall. Plant Physiol 103:1CrossRefPubMedPubMedCentralGoogle Scholar
  13. Gnanasangeetha D, SaralaThambavani D (2013) One pot synthesis of zinc oxide nanoparticles via chemical and green method. Res J Mat Sci 2320:6055Google Scholar
  14. Gogos A, Knauer K, Bucheli TD (2012) Nanomaterials in plant protection and fertilization: current state, foreseen applications, and research priorities. J Agric Food Chem 60:9781–9792CrossRefPubMedGoogle Scholar
  15. Groß F, Durner J, Gaupels F (2013) Nitric oxide, antioxidants and prooxidants in plant defence responses. Front Plant Sci 4:419CrossRefPubMedPubMedCentralGoogle Scholar
  16. Hemeda HM, Klein BP (1990) Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts. J Food Sci 55:184–185CrossRefGoogle Scholar
  17. Hoffmann C, Berganza C, Zhang J (2013) Cold atmospheric plasma: methods of production and application in dentistry and oncology. Medical Gas Res 3:1CrossRefGoogle Scholar
  18. Iranbakhsh A, Ghoranneviss M, Ardebili ZO, Ardebili NO, Tackallou SH, Nikmaram H (2017) Non-thermal plasma modified growth and physiology in Triticum aestivum via generated signaling molecules and UV radiation. Biol Plant 61(4):702–708.  https://doi.org/10.1007/s10535-016-0699-y CrossRefGoogle Scholar
  19. Iranbakhsh A, Ardebili NO, Ardebili ZO, Shafaati M, Ghoranneviss M (2018) Non-thermal plasma induced expression of Heat Shock Factor A4A and Improved Wheat (Triticum aestivum L.) growth and resistance against Salt Stress. Plasma Chem Plasma Process 38:29–44CrossRefGoogle Scholar
  20. Javed R, Usman M, Yücesan B, Zia M, Gürel E (2016) Effect of zinc oxide (ZnO) nanoparticles on physiology and steviol glycosides production in micropropagated shoots of Stevia rebaudiana Bertoni. Plant Physiol Biochem.  https://doi.org/10.1016/jplaphy201605032 PubMedGoogle Scholar
  21. Jiang J, Lu Y, Li J, Li L, He X, Shao H, Dong Y (2014) Effect of seed treatment by cold plasma on the resistance of tomato to Ralstonia solanacearum (bacterial wilt). Plos one 9:e97753CrossRefPubMedPubMedCentralGoogle Scholar
  22. Keller A, McFerran S, Lazareva A, Suh S (2013) Global life cycle releases of engineered nanomaterials. J Nanopart Res 15:1692CrossRefGoogle Scholar
  23. Landa P, Prerostova S, Petrova S, Knirsch V, Vankova R, Vanek T (2015) The transcriptomic response of arabidopsis thaliana to zinc oxide: a comparison of the impact of nanoparticle, bulk, and ionic zinc. Environ Sci Technol 49:14537–14545CrossRefPubMedGoogle Scholar
  24. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592CrossRefGoogle Scholar
  25. Lin D, Xing B (2007) Phytotoxicity of nanoparticles: inhibition of seed germination and root growth. Environ Pollut 150:243–250CrossRefPubMedGoogle Scholar
  26. Ling L, Jiafeng J, Jiangang L, Minchong S, Xin H, Hanliang S, Yuanhua D (2014) Effects of cold plasma treatment on seed germination and seedling growth of soybean. Sci Rep 4:5859.  https://doi.org/10.1038/srep05859 CrossRefPubMedPubMedCentralGoogle Scholar
  27. Ma H, Williams PL, Diamond SA (2013) Ecotoxicity of manufactured ZnO nanoparticles—a review. Environ Pollut 172:76–85CrossRefPubMedGoogle Scholar
  28. Mihai A, Dobrin D, Magurenau M, Popa M (2014) Positive effect of non-thermal plasma treatment in radish seeds. Romanian Rep Phys 66:1110–1117Google Scholar
  29. Mitra A, Li YF, Klämpfl TG, Shimizu T, Jeon J, Morfill GE, Zimmermann JL (2014) Inactivation of surface-borne microorganisms and increased germination of seed specimen by cold atmospheric plasma. Food Bioproc Technol 7:645–653CrossRefGoogle Scholar
  30. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  31. Ng KW (2011) The role of the tumor suppressor p53 pathway in the cellular DNA damage response to zinc oxide nanoparticles. Biomaterials 32:8218–8225CrossRefPubMedGoogle Scholar
  32. Peralta-Videa JR, Hernandez-Viezcas JA, Zhao L, Diaz BC, Ge Y, Priester JH, Holden PA, Gardea-Torresdey JL (2014) Cerium dioxide and zinc oxide nanoparticles alter the nutritional value of soil cultivated soybean plants. Plant Physiol Biochem 80:128–135CrossRefPubMedGoogle Scholar
  33. Safari N, Iranbakhsh A, Ardebili ZO (2017) Non-thermal plasma modified growth and differentiation process of Capsicum annuum PP805 Godiva in in vitro conditions. Plasma Sci Technol 19(5):055501CrossRefGoogle Scholar
  34. Sera B, Spatenka P, Sery M, Vrchotova N, Hruskova I (2010) Influence of plasma treatment on wheat and oat germination and early growth. IEEE Trans Plasma Sci 38:2963–2968CrossRefGoogle Scholar
  35. Sera B, Sery M, Gavril B, Gajdova I (2017) Seed germination and early growth responses to seed pretreatment by non-thermal plasma in hemp cultivars (Cannabis sativa L.). Plasma Chem Plasma Proc 37:207–221CrossRefGoogle Scholar
  36. Será B, Stranák V, Serý M, Tichý M, Spatenka P (2008) Germination of Chenopodium album in response to microwave plasma treatment. Plasma Sci Technol 10:506CrossRefGoogle Scholar
  37. Sheteiwy MS, Yajing G, Dongdong C, Jie L, Aamir N, Qijuan H, Weimin H, Mingyu N, Jin H (2015) Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress. Sci Rep 5:14278CrossRefGoogle Scholar
  38. Sheteiwy MS, Fu Y, Hu Q, Nawaz A, Guan Y, Li Z, Huang Y, Hu J (2016) Seed priming with polyethylene glycol induces antioxidative defense and metabolic regulation of rice under nano-ZnO stress. Environ Sci Poll Res 23(19):19989–20002CrossRefGoogle Scholar
  39. Sheteiwy MS, Dong Q, An J, Song W, Guan Y, He F, Huang Y, Hu J (2017) Regulation of ZnO nanoparticles-induced physiological and molecular changes by seed priming with humic acid in Oryza sativa seedlings. Plant Growth Regul 83(1):27–41CrossRefGoogle Scholar
  40. Stolárik T, Henselová M, Martinka M, Novák O, Zahoranová A, Černák M (2015) Effect of low-temperature plasma on the structure of seeds, growth and metabolism of endogenous phytohormones in pea (Pisum sativum L). Plasma Chem Plasma Process 35:659–676CrossRefGoogle Scholar
  41. Thwala M, Musee N, Sikhwivhilu L, Wepener V (2013) The oxidative toxicity of Ag and ZnO nanoparticles towards the aquatic plant Spirodela punctuta and the role of testing media parameters. Environ Sci Process Impacts 15:1830–1843CrossRefPubMedGoogle Scholar
  42. Ulbin-Figlewicz N, Jarmoluk A, Marycz K (2015) Antimicrobial activity of low-pressure plasma treatment against selected foodborne bacteria and meat microbiota. Annal Microb 65:1537–1546CrossRefGoogle Scholar
  43. Wang Y, Loake GJ, Chu C (2013) Cross-talk of nitric oxide and reactive oxygen species in plant programed cell death. Front Plant Sci 4:314PubMedPubMedCentralGoogle Scholar
  44. Wu Z, Chi L, Bian S, Xu K (2007) Effects of plasma treatment on maize seeding resistance. J Maize Sci 15:111–113Google Scholar
  45. Yang Z, Chen J, Dou R, Gao X, Mao C, Wang L (2015) Assessment of the phytotoxicity of metal oxide nanoparticles on two crop plants, maize (Zea mays L) and rice (Oryza sativa L). Int J Environ Res Public Health 12:15100–15109CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  • Alireza Iranbakhsh
    • 1
  • Zahra Oraghi Ardebili
    • 2
  • Narges Oraghi Ardebili
    • 1
  • Mahmood Ghoranneviss
    • 3
  • Nasrin Safari
    • 1
  1. 1.Department of Biology, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Department of Biology, Garmsar BranchIslamic Azad UniversityGarmsarIran
  3. 3.Plasma Physics Research Center, Science and Research BranchIslamic Azad UniversityTehranIran

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