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Abscisic acid cross-talking with hydrogen peroxide and osmolyte compounds may regulate the leaf rolling mechanism under drought

  • Asiye Sezgin
  • Cansu Altuntaş
  • Aykut Sağlam
  • Rabiye Terzi
  • Mehmet Demiralay
  • Asim Kadıoğlu
Original Article
  • 151 Downloads

Abstract

Leaf rolling observed in some crops such as maize, rice, wheat and sorghum is an indicator of decreased water status. Moderate leaf rolling not tightly or early increases the photosynthesis and grain yield of crop cultivars under environmental stresses. Moreover, the effects of exogenous abscisic acid (ABA) on stomatal conductance, water status and synthesis of osmotic compounds are a well-known issue in plants subjected to water deficit. However, it is not clear how the cross-talk of ABA with H2O2 and osmolyte compounds affects the leaf rolling mechanism. Regulation mechanism of leaf rolling by ABA has been first studied in maize seedlings under drought stress induced by polyethylene glycol 6000 (PEG 6000) in this study. ABA treatment under drought stress reduced hydrogen peroxide (H2O2) content and the degree of leaf rolling (%) while the treatment-induced ABA synthesis, osmolyte levels (proline, polyamine and total soluble sugars) and some antioxidant enzyme activities in comparison to the plants that were not treated with ABA. Furthermore, exogenous ABA up-regulated the expression levels of arginine decarboxylase (ADC) and pyrroline-5-carboxylate synthase (P5CS) genes and down-regulated polyamine oxidase (PAO), diamine oxidase (DAO) and proline dehydrogenase (ProDH) gene expressions. When endogenous ABA content was decreased by the treatment of fluoridone (FLU) that is an ABA inhibitor, leaf rolling degree (%), H2O2 content and antioxidant enzyme activities increased, but osmolyte levels, ADC and P5CS gene expressions decreased. Finally, the treatment of ABA to maize seedlings exposed to drought stress resulted in the stimulation of the antioxidant system, osmotic adjustment and reduction of leaf rolling. We concluded that ABA can be a signal compound cross-talking H2O2, proline and polyamines and thus involved in the leaf rolling mechanism by providing osmotic adjustment. The results of this study can be used to provide data for the molecular breeding of maize hybrids with high grain yield by means of moderately rolled leaves.

Keywords

ABA Drought Leaf rolling H2O2 Maize Antioxidant system Proline Polyamine Gene expression 

Abbreviations

ABA

Abscisic acid

PEG

Polyethylene glycol

H2O2

Hydrogen peroxide

ADC

Arginine decarboxylase

P5CS

Pyrroline 5-carboxylate synthase

PAO

Polyamine oxidase

DAO

Diamine oxidase

ProDH

Proline dehydrogenase

FLU

Fluoridone

MDA

Malondialdehyde

TCA

Trichloroacetic acid

NBT

Nitroblue tetrazolium

CAT

Catalase

APX

Ascorbate peroxidase

GPX

Guaiacol peroxidase

SOD

Superoxide dismutase

HPLC

High performance liquid chromatography

BR

Brassinosteroid

EDTA

Ethylenediaminetetraacetic acid

DW

Dry weight

WUE

Water use efficiency

DAB

3,3′-Diaminobenzidine

ROS

Reactive oxygen species

Notes

Acknowledgements

This work was supported by a Grant from TUBITAK (111T511).

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  • Asiye Sezgin
    • 1
  • Cansu Altuntaş
    • 1
  • Aykut Sağlam
    • 2
  • Rabiye Terzi
    • 1
  • Mehmet Demiralay
    • 3
  • Asim Kadıoğlu
    • 1
  1. 1.Department of Biology, Faculty of ScienceKaradeniz Technical UniversityTrabzonTurkey
  2. 2.Department of Molecular Biology and GeneticsKaradeniz Technical UniversityTrabzonTurkey
  3. 3.Departmant of Forest Engineering, Faculty of ForestryArtvin Coruh UniversityArtvinTurkey

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