Identification and transcriptional analysis of dehydrin gene family in cucumber (Cucumis sativus)
- 126 Downloads
Dehydrins (DHNs) are a group II late embryogenesis abundant (LEA) proteins that play essential roles in plant growth, development and responses to diverse environmental stimuli. Here, four DHNs in cucumber genome were identified using bioinformatics-based methods according to the highly conserved K-, Y- and S-segments, including 1 YnKn-type, 2 YnSKn-type, and 1 SKn-type DHNs. All of them are intrinsically disordered proteins (IDPs) and possess a large number of disorder-promoting amino acids. Secondary structure prediction revealed that each of them is composed of high proportion of alpha helix and random coil. Gene structure and phylogenetic analyses with DHNs from cucumber and several other species revealed that some closely related DHN genes had similar gene structures. A number of cis-elements involved in stress responses and phytohormones were found in each CsDHN promoter. The tissue expression profiles suggested that the CsDHN genes have overlapping, but different expression patterns. qRT-PCR results showed that three selected CsDHN genes could respond to heat, cold, osmotic and salt stresses, as well as to signaling molecules such as H2O2 and ABA. These results lay a solid foundation for future functional investigation of the cucumber dehydrin gene family in tissue development and stress responses in plants.
KeywordsCucumber Dehydrin (DHN) Gene family Intrinsically disordered protein (IDP) Expression pattern Abiotic stress
This work was financially supported by the Key Project of Youth Science Foundation of Jiangxi Province (20171ACB21025), the National Natural Science Foundation of China (31460522 and 31660578).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing financial interests.
- Peng Y, Reyes JL, Wei H, Yang Y, Karlson D, Covarrubias AA et al (2008) RcDhn5, a cold acclimation-responsive dehydrin from Rhododendron catawbiense rescues enzyme activity from dehydration effects in vitro and enhances freezing tolerance in RcDhn5-overexpressing Arabidopsis plants. Physiol Plant 134:583–597CrossRefPubMedGoogle Scholar
- Zhou Y, Hu L, Wu H, Jiang L, Liu S (2017c) Genome-wide identification and transcriptional expression analysis of cucumber superoxide dismutase (SOD) family in response to various abiotic stresses. Int J Genom 2017:7243973Google Scholar