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SOC1 and AGL24 interact with AGL18-1, not the other family members AGL18-2 and AGL18-3 in Brassica juncea

  • Chao-chuang Li
  • Guan-peng Ma
  • Ting Xie
  • Jiao Chen
  • Zhi-min Wang
  • Ming Song
  • Qing-lin TangEmail author
Original Article

Abstract

Many MCM1-AGAMOUS-DEFICIENS-SRF (MADS) genes have been proved to play an important role in the flowering time regulation of plants. The flowering-inhibiting factor AGAMOUS-LIKE 18 (AGL18) integrates into the two flowering-activating factors SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and AGAMOUS-LIKE 24 (AGL24), which play an important role during the plant developmental stages of the flowering pathway. However, it remains unknown whether and how the AGL18 protein directly interacts with SOC1 and/or AGL24 genes to regulate flowering time in Brassica juncea. In this study, three members (AGL18-1 in florescence, AGL18-2 and AGL18-3 in young seedlings) of the AGL18 family, and SOC1 and AGL24 in florescence were cloned in Brassica juncea. Yeast One-Hybrid assays and Dual-Glo® Luciferase assays showed that the SOC1 and AGL24 promoters interacted only with AGL18-1 protein, not AGL18-2 and AGL18-3. The typical conserved structure of the M-domain of AGL18-1 was the key region that mediated the interaction between the AGL18-1 protein and SOC1 promoter, and the I-domain, K-domain and C-domain did not regulate the interaction of AGL18-1/SOC1. In contrast, the K-domain and M-domain in AGL18-1 could mediate the interaction between the AGL18-1 protein and AGL24 promoter. This indicated that the AGL18-1 protein must have its unique functions that differed from AGL18-2 and AGL18-3. This work provides valuable information for in-depth studies into the molecular mechanisms of the AGL18 protein with SOC1 and AGL24 for flowering time control of Brassica juncea.

Keywords

Brassica juncea AGL18 SOC1 AGL24 Flowering time control 

Notes

Acknowledgements

This work was financially supported by Grants from the National Program on Key Basic Research Projects (The 973 Program: 2012CB113900), the National Natural Science Foundation of China (31000908), the Chongqing Natural Science Foundation (2011BA1002) and the Fundamental Research Funds for the Central Universities (XDJK2017B036).

Supplementary material

11738_2017_2580_MOESM1_ESM.docx (20 kb)
Supplementary material 1 (DOCX 20 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2017

Authors and Affiliations

  • Chao-chuang Li
    • 1
  • Guan-peng Ma
    • 1
    • 2
  • Ting Xie
    • 1
    • 3
  • Jiao Chen
    • 1
  • Zhi-min Wang
    • 1
  • Ming Song
    • 1
  • Qing-lin Tang
    • 1
    Email author
  1. 1.College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqingChina
  2. 2.Guizhou Province Horticulture Research InstituteGuiyangChina
  3. 3.Bijie Station for Popularizing Agricultural TechniqueBijieChina

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