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Frontiers of Materials Science

, Volume 12, Issue 4, pp 438–446 | Cite as

Electrospun polypyrrole-coated polycaprolactone nanoyarn nerve guidance conduits for nerve tissue engineering

  • Xin Pan
  • Binbin SunEmail author
  • Xiumei MoEmail author
Research Article
  • 20 Downloads

Abstract

Nerve guidance conduits (NGCs) can provide suitable microenvironment for nerve repair and promote the proliferation and migration of Schwann cells (SCs). Thus, we developed nerve guidance conduits (NGCs) with polypyrrole-coated polycaprolactone nanoyarns (PPy-PCL-NYs) as fillers in this study. PCL-NYs with the oriented structure were prepared with a double-needle electrospinning system and then PPy was coated on PCL-NYs via the in situ chemical polymerization. Subsequently, PCL nanofibers were collected around nanoyarns by the conventional electrospinning process as the outer layer to obtain PPy-PCL-NY nerve guidance conduits (PPy-PCLNY NGCs). PPy-PCL-NYs were analyzed by SEM, FTIR and XPS. Results showed that PPy was homogeneously and uniformly deposited on the surface of PCL-NY. Strain-stress curves and the Young’s modulus of PPy-PCL-NYs were investigated compared with those of non-coated PCL-NYs. Studies on biocompatibility with SCs indicated that PPy-PCL-NY NGCs were more conducive to the proliferation of SCs than PCL-NY NGCs. In summary, PPy-PCL-NY NGCs show the promising potential for nerve tissue engineering repair and regeneration.

Keywords

electrospinning Schwann cell (SC) polypyrrole (PPy) polycaprolactone nanoyarn (PCL-NY) nerve guidance conduit (NGC) 

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Notes

Acknowledgements

This research was supported by the National Key Research Program of China (2016YFA0201702 of 2016YFA0201700) and the National Natural Science Foundation of China (Grant Nos. 31771023 and 81802131).

References

  1. [1]
    Dvali L T, Myckatyn T M. End-to-side nerve repair: review of the literature and clinical indications. Hand Clinics, 2008, 24(4): 455–460CrossRefGoogle Scholar
  2. [2]
    Li X, Yang Z, Zhang A, et al. Repair of thoracic spinal cord injury by chitosan tube implantation in adult rats. Biomaterials, 2009, 30(6): 1121–1132CrossRefGoogle Scholar
  3. [3]
    Yao L, de Ruiter G C, Wang H, et al. Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit. Biomaterials, 2010, 31(22): 5789–5797CrossRefGoogle Scholar
  4. [4]
    Ribeiro-Resende V T, Koenig B, Nichterwitz S, et al. Strategies for inducing the formation of bands of Büngner in peripheral nerve regeneration. Biomaterials, 2009, 30(29): 5251–5259CrossRefGoogle Scholar
  5. [5]
    Tsujimoto H, Nakamura T, Miki T, et al. Regeneration and functional recovery of intrapelvic nerves removed during extensive surgery by a new artificial nerve conduit: a breakthrough to radical operation for locally advanced and recurrent rectal cancers. Journal of Gastrointestinal Surgery, 2011, 15(6): 1035–1042CrossRefGoogle Scholar
  6. [6]
    Wang H B, Mullins M E, Cregg J M, et al. Varying the diameter of aligned electrospun fibers alters neurite outgrowth and Schwann cell migration. Acta Biomaterialia, 2010, 6(8): 2970–2978CrossRefGoogle Scholar
  7. [7]
    Hanna A S, Son Y J, Dempsey R. Live imaging of dorsal root regeneration and the resurgence of a forgotten idea. Neurosurgery, 2011, 69(2): N18–N21Google Scholar
  8. [8]
    Xie J, MacEwan MR, Schwartz A G, et al. Electrospun nanofibers for neural tissue engineering. Nanoscale, 2010, 2(1): 35–44CrossRefGoogle Scholar
  9. [9]
    Gelain F, Unsworth L D, Zhang S. Slow and sustained release of active cytokines from self-assembling peptide scaffolds. Journal of Controlled Release, 2010, 145(3): 231–239CrossRefGoogle Scholar
  10. [10]
    Madduri S, Papaloïzos M, Gander B. Trophically and topographically functionalized silk fibroin nerve conduits for guided peripheral nerve regeneration. Biomaterials, 2010, 31(8): 2323–2334CrossRefGoogle Scholar
  11. [11]
    Yin Z, Chen X, Chen J L, et al. The regulation of tendon stem cell differentiation by the alignment of nanofibers. Biomaterials, 2010, 31(8): 2163–2175CrossRefGoogle Scholar
  12. [12]
    Lundborg G, Rosen B, Dahlin L, et al. Tubular versus conventional repair of median and ulnar nerves in the human forearm: Early results from a prospective, randomized, clinical study. The Journal of Hand Surgery, 1997, 22(1): 99–106CrossRefGoogle Scholar
  13. [13]
    Yucel D, Kose G T, Hasirci V. Polyester based nerve guidance conduit design. Biomaterials, 2010, 31(7): 1596–1603CrossRefGoogle Scholar
  14. [14]
    Li D, Pan X, Sun B, et al. Nerve conduits constructed by electrospun P(LLA-CL) nanofibers and PLLA nanofiber yarns. Journal of Materials Chemistry B: Materials for Biology and Medicine, 2015, 3(45): 8823–8831CrossRefGoogle Scholar
  15. [15]
    Urbanek O, Sajkiewicz P, Pierini F. The effect of polarity in the electrospinning process on PCL/chitosan nanofibres’ structure, properties and efficiency of surface modification. Polymer, 2017, 124: 168–175CrossRefGoogle Scholar
  16. [16]
    Guimard N K, Gomez N, Schmidt C E. Conducting polymers in biomedical engineering. Progress in Polymer Science, 2007, 32(8): 876–921CrossRefGoogle Scholar
  17. [17]
    Guo B, Glavas L, Albertsson A C. Biodegradable and electrically conducting polymers for biomedical applications. Progress in Polymer Science, 2013, 38(9): 1263–1286CrossRefGoogle Scholar
  18. [18]
    Zhang J, Qiu K, Sun B, et al. The aligned core–sheath nanofibers with electrical conductivity for neural tissue engineering. Journal of Materials Chemistry B: Materials for Biology and Medicine, 2014, 2(45): 7945–7954CrossRefGoogle Scholar
  19. [19]
    Lee J Y, Bashur C A, Goldstein A S, et al. Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications. Biomaterials, 2009, 30(26): 4325–4335CrossRefGoogle Scholar
  20. [20]
    Runge M B, Dadsetan M, Baltrusaitis J, et al. The development of electrically conductive polycaprolactone fumarate-polypyrrole composite materials for nerve regeneration. Biomaterials, 2010, 31(23): 5916–5926CrossRefGoogle Scholar
  21. [21]
    Schmidt C E, Shastri V R, Vacanti J P, et al. Stimulation of neurite outgrowth using an electrically conducting polymer. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(17): 8948–8953CrossRefGoogle Scholar
  22. [22]
    Xie J, Macewan M R, Willerth S M, et al. Conductive core–sheath nanofibers and their potential application in neural tissue engineering. Advanced Functional Materials, 2009, 19(14): 2312–2318CrossRefGoogle Scholar
  23. [23]
    Sun B, Wu T, Wang J, et al. Polypyrrole-coated poly(l-lactic acidco-ε-caprolactone)/silk fibroin nanofibrous membranes promoting neural cell proliferation and differentiation with electrical stimulation. Journal of Materials Chemistry B: Materials for Biology and Medicine, 2016, 4(41): 6670–6679CrossRefGoogle Scholar
  24. [24]
    Wu T, Li D, Wang Y, et al. Laminin-coated nerve guidance conduits based on poly(l-lactide-co-glycolide) fibers and yarns for promoting Schwann cells’ proliferation and migration. Journal of Materials Chemistry B: Materials for Biology and Medicine, 2017, 5(17): 3186–3194CrossRefGoogle Scholar
  25. [25]
    Levitt A S, Knittel C E, Vallett R, et al. Investigation of nanoyarn preparation by modified electrospinning setup. Journal of Applied Polymer Science, 2017, 134(19): 44813CrossRefGoogle Scholar
  26. [26]
    Liu P, Wu S, Zhang Y, et al. A fast response ammonia sensor based on coaxial PPy-PAN nanofiber yarn. Nanomaterials, 2016, 6 (7): E121 (10 pages)Google Scholar
  27. [27]
    Ichihara S, Inada Y, Nakamura T. Artificial nerve tubes and their application for repair of peripheral nerve injury: an update of current concepts. Injury, 2008, 39(39 Suppl 4): 29–39Google Scholar
  28. [28]
    Matsumoto K, Ohnishi K, Kiyotani T, et al. Peripheral nerve regeneration across an 80-mm gap bridged by a polyglycolic acid (PGA)-collagen tube filled with laminin-coated collagen fibers: a histological and electrophysiological evaluation of regenerated nerves. Brain Research, 2000, 868(2): 315–328CrossRefGoogle Scholar
  29. [29]
    Mo X, Li D, Ei-Hamshary H A, et al. Electrospinning nanofibers for tissue engineering. Journal of Fiber Bioengineering and Informatics, 2013, 6(3): 225–235CrossRefGoogle Scholar
  30. [30]
    Lietz M, Dreesmann L, Hoss M, et al. Neuro tissue engineering of glial nerve guides and the impact of different cell types. Biomaterials, 2006, 27(8): 1425–1436CrossRefGoogle Scholar

Copyright information

© Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Orthopaedics, Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiChina
  2. 2.Sunna Technologies (Shanghai) Co., Ltd.ShanghaiChina
  3. 3.State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and BiotechnologyDonghua UniversityShanghaiChina

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