Effect of incorporating Elaeagnus angustifolia extract in PCL-PEG-PCL nanofibers for bone tissue engineering

  • Vahideh R. Hokmabad
  • Soodabeh Davaran
  • Marziyeh Aghazadeh
  • Effat Alizadeh
  • Roya SalehiEmail author
  • Ali RamazaniEmail author
Research Article


Plants have been used for medicinal purposes for thousands of years but they are still finding new uses in modern times. For example, Elaeagnus angustifolia (EA) is a medicinal herb with antinociceptive, anti-inflammatory, antibacterial and antioxidant properties and it is widely used in the treatment of rheumatoid arthritis and osteoarthritis. EA extract was loaded onto poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) (PCL-PEG-PCL/EA) nanofibers and their potential applications for bone tissue engineering were studied. The morphology and chemical properties of the fibers were evaluated using Fourier transform infrared spectroscopy, field emission scanning electron microscopy, contact angle measurements and mechanical tests. All the samples had bead-free morphologies with average diameters ranging from 100 to 200 nm. The response of human cells to the PCL-PEG-PCL/EA nanofibers was evaluated using human dental pulp stem cells (hDPSCs). The hDPSCs had better adhesion and proliferation capacity on the EA loaded nanofibers than on the pristine PCL-PEG-PCL nanofibers. An alizarin red S assay and the alkaline phosphatase activity confirmed that the nanofibrous scaffolds induced osteoblastic performance in the hDPSCs. The quantitative real time polymerase chain reaction results confirmed that the EA loaded nanofibrous scaffolds had significantly upregulated gene expression correlating to osteogenic differentiation. These results suggest that PCL-PEG-PCL/EA nanofibers might have potential applications for bone tissue engineering.


Elaeagnus angustifolia scaffold electrospinning human dental pulp stem cell tissue engineering 


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The authors were financially supported by a grant (No. 94/104) from the Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.


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Copyright information

© Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Vahideh R. Hokmabad
    • 1
  • Soodabeh Davaran
    • 2
  • Marziyeh Aghazadeh
    • 3
    • 4
  • Effat Alizadeh
    • 3
    • 5
  • Roya Salehi
    • 2
    Email author
  • Ali Ramazani
    • 1
    Email author
  1. 1.Department of ChemistryUniversity of ZanjanZanjanIran
  2. 2.Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical ScienceTabriz University of Medical SciencesTabrizIran
  3. 3.Stem Cell Research CenterTabriz University of Medical SciencesTabrizIran
  4. 4.Oral Medicine Department of Dental FacultyTabriz University of Medical SciencesTabrizIran
  5. 5.Department of Medical Biotechnology, Faculty of Advanced Medical ScienceTabriz University of Medical SciencesTabrizIran

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