A green method to the preparation of the silver-loaded diatomite with enhanced antibacterial properties

  • JiXi Chen
  • JunQiu Zhu
  • ShuiYuan LuoEmail author
  • XiaoXiao Zhong
Original Paper


The commonly diatomite was selected as the substrate materials for supporting Ag nano-particles due to its high-temperature resistance and low cost. The diatomite adsorption Ag+ from an electrolytic cell, which cathode and anode are Ti and Ag, respectively. After being thermal treatment, Ag+ was successfully changed to metallic Ag nano-particle. Experimental studies revealed that the thermal treatment temperature plays an important role in controlling the size and the concentration of Ag particle. The SEM, XRD, and XPS results showed that Ag particles are deposited on the surface of diatomite and the particle size of the silver is about 50 ~ 90 nm. Antibacterial tests indicated that the diatomite supporting Ag nano-particle exhibits excellent antibacterial against Gram-negative E. Coli and Gram-positive S. aureus, mainly attributed to Ag particle immediately collapse the micro-organisms by disrupting the biological function of the cell membranes.


Antibacterial diatomite Silver nano-particles Electrochemical Thermal reduction 



This work was supported by The QuanZhou science and technology bureau, Numbers.2017G017.


  1. Albrecht MA, Evan CW, Raston CL (2006) Green chemistry and the health implications of nanoparticles. Green Chem 8:417–432CrossRefGoogle Scholar
  2. Ali T, Ateeq A, Umair A, Imran U et al (2018) Enhanced photocatalytic and antibacterial activities of Ag-doped TiO2 nanoparticles under visible light. Mater Chem Phys 212:325–335CrossRefGoogle Scholar
  3. Baheiraei N, Moztaradeh F, Hedayati M (2012) Preparation and antibacterial activity of Ag/SiO2 thin film on glazed ceramic tiles by sol–gel method. Ceram Int 38:5CrossRefGoogle Scholar
  4. Burd A, Kwok CH, Hung SC, Chan HS, Gu H, Lam WK et al (2007) A comparative study of the cytotoxicity of silver-based dressings in monolayer cell, tissue explant, and animal models. Wound Repair Regen 15:94–104CrossRefGoogle Scholar
  5. Castellano JJ, Shafii SM, Ko F, Donate G, Wright TE, Mannari RJ et al (2007) Comparative evaluation of silver-containing antimicrobial dressings and drugs. Int Wound J 4(2):114–122CrossRefGoogle Scholar
  6. Chen X, Liu X, Huang K (2018) Large-scale synthesis of size-controllable Ag nanoparticles by reducing silver halide colloids with different sizes. Chin Chem Lett 30(3):797–800CrossRefGoogle Scholar
  7. Duan C, Meng J, Wang X, Meng X, Sun X, Xu Y et al (2018) Synthesis of novel cellulose- based antibacterial composites of Ag nanoparticles@ metal-organic frameworks@ carboxymethylated fibers. Carbohyd Polym 193:82–88CrossRefGoogle Scholar
  8. Gvidona P, Shevchenko E, Vashchanka Svyatlana V et al (2008) On the nature of the processes occurring in silver doped SiO2 films under heat treatment. J Sol-Gel Sci Technol 45:143–149CrossRefGoogle Scholar
  9. He X, Wang F, Liu H, Li J et al (2017) Synthesis of quartz crystals supporting Ag nanoparticle powder with enhanced antibacterial properties. Surf Interfaces 6:122–126CrossRefGoogle Scholar
  10. Hilonga A, Kim J-K, Sarawade PB et al (2010) Influence of annealing conditions on the properties of reinforced silver-embedded silica matrix from the cheap silica source. Appl Surf Sci 256:2849–2855CrossRefGoogle Scholar
  11. Hilonga A, Kim J-K, Sarawade PB et al (2012) Silver-doped silica powder with an-tibacterial properties. Powder Technol 215–216:219–222CrossRefGoogle Scholar
  12. Huang J, Zhou J, Zhuang J et al (2017) Strong near-infrared absorbing and biocompatible CuS nanoparticles for rapid and efficient photothermal ablation of gram-positive and -negative bacteria. ACS Appl Mater Interfaces 9(42):36606–36614CrossRefGoogle Scholar
  13. Johari SA, Sarkheil M, Behzadi Tayemeh M, Veisi S (2018) Influence of salinity on the toxicity of silver nanoparticles (AgNPs) and silver nitrate (AgNO3) in halophilic microalgae, Dunaliella salina. Chemosphere 209:156–162CrossRefGoogle Scholar
  14. Kaushal I, Saharan P, Kumar V, Sharma AK, Umar A (2019) Superb sono-adsorption and energy storage potential of multifunctional Ag-Biochar composite. J Alloy Compd 785:240–249CrossRefGoogle Scholar
  15. Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ et al (2007) Antimicrobial effects of silver nanoparticles. Nanomed Nanotechnol Biol Med 3:95–101CrossRefGoogle Scholar
  16. Kishore M, Hanumantha Rao Y (2018) Green synthesis and characterization of silver nanoparticle from ampelocissus latifolia root extract. Mater Today Proceed 5(13, Part 1):26271–26279CrossRefGoogle Scholar
  17. Kumar H, Gaur A, Kumar S, Park JW (2019) Development of silver nanoparticles-loaded CMC hydrogel using bamboo as a raw material for special medical applications. Chem Pap 73(4):953–964CrossRefGoogle Scholar
  18. Lin L, Zhang H, Cui H et al (2013) Preparation and antibacterial activities of hollow silica–Ag sphere. Colloids Surf B Biointerf 101:97–100CrossRefGoogle Scholar
  19. Liu G, Haiqi G, Li K, Xiang J, Lan T, Zhang Z (2018) Fabrication of silver nanoparticle sponge leather with durable antibacterial property. J Colloid Interface Sci 514:338–348CrossRefGoogle Scholar
  20. Ma Y, Liu C, Qu D, Chen Y, Huang M, Liu Y (2017) Antibacterial evaluation of sliver nanoparticles synthesized by polysaccharides from Astragalus membranaceus roots. Biomed Pharmacother 89:351–357CrossRefGoogle Scholar
  21. Maddah B, Shamsi J, Barsang MJ, Rahimi-Nasrabadi M (2015) The chemiluminescence determination of 2-chloroethyl ethyl sulfide using luminol–AgNO3–silver nanoparticles system. Spectrochim Acta Part A Mol Biomol Spectrosc 142:220–225CrossRefGoogle Scholar
  22. Maddinedi SB, Mandal BK, Maddili SK (2017) Biofabrication of size controllable silver nanoparticles–A green approach. J Photochem Photobiol B Biol 167:236–241CrossRefGoogle Scholar
  23. Min HK, Donghwan C, oh HK, Won HP (2018) Thermal fabrication and characterization of Ag nanoparticle-activated carbon composites for functional wound-dressing additives. J Alloy Compd 735:2670–2674CrossRefGoogle Scholar
  24. Misran H, Salim MA, Ramesh S (2018) Effect of Ag nanoparticles seeding on the properties of silica spheres. Ceram Int 44(6):5901–5908CrossRefGoogle Scholar
  25. Mohapatra B, Kumar D, Sharma N, Mohapatra S (2019) Morphological, plasmonic and enhanced antibacterial properties of Ag nanoparticles prepared using Zingiber officinale extract. J Phys Chem Solids 126:257–266CrossRefGoogle Scholar
  26. Mwhrdad K, Rajender S, Niloofar Z et al (2018) Applications of green synthesized Ag, ZnO and Ag/ZnO nanoparticles for making clinical antimicrobial wound-healing bandages. Sustain Chem Pharm 10:9–15CrossRefGoogle Scholar
  27. Pallavicini P, Dacarro G, Taglietti A (2018) Self-assembled monolayers of silver nanoparticles: from intrinsic to switchable inorganic antibacterial surfaces. Eur J Inorg Chem 45:4846–4855CrossRefGoogle Scholar
  28. Pan KY, Liang YF, Pu YC, Hsu YJ, Yeh JW, Shih HC (2014) Studies on the photocatalysis of core-shelled SiO2–Ag nanospheres by controlled surface plasmon resonance under visible light. Appl Surf Sci 311:399–404CrossRefGoogle Scholar
  29. Panda SK, Sen S, Roy S, Moyez A (2018) Synthesis of colloidal silver nanoparticles by reducing aqueous AgNO3 Using Green Reducing Agents. Mater Today Proceed 5(3, Part 3):10054–10061CrossRefGoogle Scholar
  30. Prieto P, Nistor V, Nouneh K, Oyama M, Lefdil MA, Díaz R (2012) XPS study of silver, nickel and bimetallic silver–nickel nanoparticles prepared by seedmediated growth. Appl Surf Sci 258:8807–8813CrossRefGoogle Scholar
  31. Raghavendra GM, Jung J, Kim D, Varaprasad K, Seo J (2016) Identification of silver cubic structures during ultrasonication of chitosan AgNO3 solution. Carbohyd Polym 152:558–565CrossRefGoogle Scholar
  32. Salim MA, Misran H, Othman SZ, Mahadi N, Pauzi NIM, Manap A (2015) Synthesis and characterizations of SiO2-Ag core-shell nanostructure using fatty alcohols as surface modifiers. Appl Mech Mater 773–774:199–203CrossRefGoogle Scholar
  33. Stejskal J, Trchová M, Kovářová J, Prokeš J, Omastová M (2008) Polyaniline-coated cellulose fibers decorated with silver nanoparticles. Chem Pap 62(2):181–186CrossRefGoogle Scholar
  34. Stejskal J, Trchová M, Brožová L, Prokeš J (2009) Reduction of silver nitrate by polyaniline nanotubes to produce silver-polyaniline composites. Chem Pap 63(1):77–83CrossRefGoogle Scholar
  35. Sudrik Surendra G, Chaki Nirmalya K, Chavan Vilas B et al (2006) Silver nanocluster redox-couple-promoted nonclassical electron transfer: an efficient electrochemical wolff rearrangement of α-Diazoketones. Chem A Eur J 12:859–864CrossRefGoogle Scholar
  36. Thi Ngoc Tu Le, Nu Quynh Trang Ton, et al (2017) TiO2 nanotubes with different Ag loading to enhance visible-light photocatalytic activity. Journal of Nanomaterials. 2017, Article ID 6092195, 7 pagesGoogle Scholar
  37. Tripathi A, Liu S, Singh PK, Kumar N, Pandey AC, Tripathi DK et al (2017) Differential phytotoxic responses of silver nitrate (AgNO3) and silver nanoparticle (AgNps) in Cucumis sativus L. Plant Gene 11:255–264CrossRefGoogle Scholar
  38. Vilchis-Nestor Alfredo R, Sánchez-Mendieta Victor, Camacho-López Marco A et al (2008) Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract. Mater Lett 62:3103–3105CrossRefGoogle Scholar
  39. Muthu K, Priya S (2017) Green synthesis, characterization and catalytic activity of silver nanoparticles using Cassia auriculata flower extract separated fraction. Spectrochim Acta Mol Biomol Spectrosc 179:66–72CrossRefGoogle Scholar
  40. Yunping Wu, Yang Yan, Zhang Zhijie, Wang Zhihua et al (2018) A facile method to prepare size-tunable silver nanoparticles and its antibacterial mechanism. Adv Powder Technol 29(2):407–415CrossRefGoogle Scholar
  41. Zeyuan Z, Shuxiang Y, Yuan L, Ming Z, Hanhui C, Hongtao S et al (2011) Effect of AgNO3 concentration on morphologies and structures of nanostructured Ag. Rare Metal Mater Engg 40(2):215–219CrossRefGoogle Scholar
  42. Zhang Yongwen, Peng Huashong, Huang Wei et al (2008) Hyperbranched poly(amidoamine) as the stabilizer and reductant to prepare colloid silver nanoparticles in situ and their antibacterial activity. J Phys Chem C 112:2330–2336CrossRefGoogle Scholar
  43. Zhang X, Sun H, Tan S, Gao J, Fu Y, Liu Z (2019) Hydrothermal synthesis of Ag nanoparticles on the nanocellulose and their antibacterial study. Inorg Chem Commun 100:44–50CrossRefGoogle Scholar
  44. Zheng R, Ren Z, Gao Huimin et al (2018) Effects of calcination on silica phase transition in diatomite. J Alloy Compd 757:364–371CrossRefGoogle Scholar

Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Fujian Engineering and Research Center of Green and Environment-Friendly Functional Footwear MaterialsQuanZhou Normal UniversityQuanzhouChina
  2. 2.School of Materials and Chemical EngineeringQuanZhou Normal UniversityQuanzhouChina

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