Chemical Papers

, Volume 74, Issue 2, pp 459–470 | Cite as

Bio-ceramic, mesoporous cuttlebone of Sepia officinalis is an ideal support for the immobilization of Bacillus subtilis AKL13 lipase: optimization, adsorption, thermodynamic and reaction kinetic studies

  • Karthikumar Sankar
  • Anant AcharyEmail author
Original Paper


Mesoporous cuttlebone powder of Sepia officinalis (CBP) was characterized and used as matrix for the immobilization of Bacillus subtilis AKL 13 lipase (BsL). Particle size and surface area of the matrix used for enzyme immobilization were 89.95 µm and 1.631 m2 g−1, respectively. Remarkable thermostability (54% of weight loss at 700 °C) of CBP was determined in TGA profile. The lipase immobilization process parameters were sequentially optimized by response surface methodology followed by artificial neural network and genetic algorithm. The maximum lipase loading capacity of CBP was 255 mg g−1 of support. Immobilized lipase (CBP-BsL) showed maximum specific activity of 5808 U mg−1 of protein in p-nitrophenol palmitate hydrolysis. Adsorption isotherm study revealed that the binding of lipase on the surface of CBP was Langmuir and the binding was physical adsorption. CBP-BsL showed lower activation energy (51.4 KJ mol−1) and higher thermal stability with half-lives of 13.3 h at 50 °C. Higher activity retention in nonpolar solvents and 69% of operational stability after 15 cycle of reaction were measured.


Sepia officinalis Cuttlebone Bacillus subtilis AKL 13 Lipase Immobilization Optimization 



The authors gratefully acknowledge the management of Kamaraj College of Engineering and Technology, Virudhunagar-626001, Tamil Nadu, India, for the support of research facilities.

Supplementary material

11696_2019_891_MOESM1_ESM.docx (165 kb)
Supplementary material 1 (DOCX 164 kb)


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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Department of BiotechnologyKamaraj College of Engineering and TechnologyMaduraiIndia

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