Nitrogen-doped reduced graphene oxide as a sensing platform for detection of guanine and application in cell necrosis
In this paper, a novel electrochemical sensor was fabricated based on nitrogen-doped reduced graphene oxide (N-RGO). The morphology, structure and electrochemical properties of N-RGO were investigated by transmission electron microscopy (TEM), atomic force microscope (AFM), X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry (CV). AFM indicated the thickness of N-RGO is about 1.0 nm. The electrochemical experiments demonstrated that N-RGO possesses a relatively large surface area, strong adsorptive ability and excellent electrical conductivity. It was believed that N-RGO is a promising candidate for applications in electrochemical sensors and biosensors. Moreover, N-RGO modified glassy carbon electrode (N-RGO/GCE) exhibited good electrochemical response toward the oxidation of guanine with a linear range covering 4.14 × 10−7–3.71 × 10−4 M, and the corresponding detection limit (LOD) of 1.38 × 10−7 M. Eventually, the proposed sensor could be used to monitor cell necrosis by means of detecting the increase of the current response of guanine. Thus, the work is very meaningful in the field of biological cytology and pathology.
KeywordsNitrogen Doped reduced graphene oxide Cell contents Guanine Cell necrosis Cyclic voltammetry
This work was supported by the National Natural Science Foundation of China (Nos. 21565021).
Compliance with ethical standards
Conflict of interest
The authors declare no competing financial interest.
- Atchudan R, Edison TN, Perumal S, Shanmugam M, Lee YR (2017b) Direct solvothermal synthesis of zinc oxide nanoparticle decorated graphene oxide nanocomposite for efficient photodegradation of azo-dyes. J Photochem Photobiol A337:100–111. https://doi.org/10.1016/j.jphotochem.2017.01.021 CrossRefGoogle Scholar
- Atchudan R, Jebakumar TN, Edison I, Perumal S, Karthikeyan D, Lee YR (2017c) Effective photocatalytic degradation of anthropogenic dyes using graphene oxide grafting titanium dioxide nanoparticles under UV-light irradiation. J Photochem Photobiol, A 333:92–104. https://doi.org/10.1016/j.jphotochem.2016.10.021 CrossRefGoogle Scholar
- Atchudan R, Edison TN, Chakradhar D, Karthik N, Perumal S, Lee YR (2018a) One-pot dual product synthesis of hierarchical Co3O4@N-rGO for supercapacitors, N-GDs for label-free detection of metal ion and bio-imaging applications. Ceram Int 44:2869–2883. https://doi.org/10.1016/j.ceramint.2017.11.034 CrossRefGoogle Scholar
- Atchudan R, Edison TN, Perumal S, Karthik N, Karthikeyan D, Shanmugam M, Lee YR (2018b) Concurrent synthesis of nitrogen-doped carbon dots for cell imaging and ZnO@nitrogen-doped carbon sheets for photocatalytic degradation of methylene blue. J Photochem Photobiol, A 350:75–85. https://doi.org/10.1016/j.jphotochem.2017.09.038 CrossRefGoogle Scholar
- Bonfoco E, Krainc D, Ankarcrona M, Nicotera P, Lipton SA (1995) Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci 92:7162–7166. https://doi.org/10.1073/pnas.92.16.7162 CrossRefGoogle Scholar
- Giribabu K, Suresh R, Manigandan R, Kumar SP, Muthamizh S, Munusamy S, Narayanan V (2014) Preparation of nitrogen-doped reduced graphene oxide and its use in a glassy carbon electrode for sensing 4-nitrophenol at nanomolar levels. Microchim Acta 181:1863–1870. https://doi.org/10.1007/s00604-014-1251-4 CrossRefGoogle Scholar
- Lemasters JJ, Nieminen A, Qian T, Trost LC, Elmore SP, Nishimura Y, Crowe RA, Cascio WE, Bradham CA, Brenner DA, Herman B (1998) The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy. Biochim Biophys Acta 1366:177–196. https://doi.org/10.1016/S0005-2728(98)00112-1 CrossRefGoogle Scholar
- Li HY, Wang XL, Yu ZY (2014) Electrochemical biosensor for sensitively simultaneous determination of dopamine, uric acid, guanine, and adenine based on poly-melamine and nano Ag hybridized film-modified electrode. J Solid State Electrochem 18:105–113. https://doi.org/10.1007/s10008-013-2242-9 CrossRefGoogle Scholar
- Usachov D, Vilkov O, Gruneis A, Haberer D, Fedorov A, Adamchuk VK, Preobrajenski AB, Dudin P, Barinov A, Oehzelt M, Laubschat C, Vyalik DV (2011) Nitrogen-doped graphene: efficient growth, structure, and electronic properties. Nano Lett 11:5401–5407. https://doi.org/10.1021/nl2031037 CrossRefGoogle Scholar
- Wang HB, Zhang HD, Xu LL, Gan T, Huang KJ, Liu YM (2014) Electrochemical biosensor for simultaneous determination of guanine and adenine based on dopamine-melanin colloidal nanospheres-graphene composites. J Solid State Electrochem 18:2435–2442. https://doi.org/10.1007/s10008-014-2494-z CrossRefGoogle Scholar