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Nitrogen-doped reduced graphene oxide as a sensing platform for detection of guanine and application in cell necrosis

  • Guangjie Song
  • Rongjin Zhang
  • Xuechan Jiang
  • Fuxin Liu
  • Xiuhui LiuEmail author
Original Paper

Abstract

In this paper, a novel electrochemical sensor was fabricated based on nitrogen-doped reduced graphene oxide (N-RGO). The morphology, structure and electrochemical properties of N-RGO were investigated by transmission electron microscopy (TEM), atomic force microscope (AFM), X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry (CV). AFM indicated the thickness of N-RGO is about 1.0 nm. The electrochemical experiments demonstrated that N-RGO possesses a relatively large surface area, strong adsorptive ability and excellent electrical conductivity. It was believed that N-RGO is a promising candidate for applications in electrochemical sensors and biosensors. Moreover, N-RGO modified glassy carbon electrode (N-RGO/GCE) exhibited good electrochemical response toward the oxidation of guanine with a linear range covering 4.14 × 10−7–3.71 × 10−4 M, and the corresponding detection limit (LOD) of 1.38 × 10−7 M. Eventually, the proposed sensor could be used to monitor cell necrosis by means of detecting the increase of the current response of guanine. Thus, the work is very meaningful in the field of biological cytology and pathology.

Graphic abstract

A sensitive sensor for guanine detection is constructed based on nitrogen-doped reduced graphene oxide (N-RGO), which was synthesized by a facile method. This paper is the first time to monitor cell necrosis by detecting the increase of guanine content.

Keywords

Nitrogen Doped reduced graphene oxide Cell contents Guanine Cell necrosis Cyclic voltammetry 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 21565021).

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interest.

Supplementary material

11696_2019_856_MOESM1_ESM.docx (3 mb)
The calculation of the electrochemical effective surface area and the saturating absorption capacity of bare electrode and N-RGO modified electrode, the optimization of experimental variables, the study of reproducibility, interference immunity and stability of the modified electrode, the control experiment of monitoring cell necrosis and the comparison of various sensors. (DOCX 3056 kb)

References

  1. Atchudan R, Edison TN, Perumal S, Lee YR (2017a) Green synthesis of nitrogen-doped graphitic carbon sheets with use of Prunus persica for supercapacitor applications. Appl Surf Sci 393:276–286.  https://doi.org/10.1016/j.apsusc.2016.10.030 CrossRefGoogle Scholar
  2. Atchudan R, Edison TN, Perumal S, Shanmugam M, Lee YR (2017b) Direct solvothermal synthesis of zinc oxide nanoparticle decorated graphene oxide nanocomposite for efficient photodegradation of azo-dyes. J Photochem Photobiol A337:100–111.  https://doi.org/10.1016/j.jphotochem.2017.01.021 CrossRefGoogle Scholar
  3. Atchudan R, Jebakumar TN, Edison I, Perumal S, Karthikeyan D, Lee YR (2017c) Effective photocatalytic degradation of anthropogenic dyes using graphene oxide grafting titanium dioxide nanoparticles under UV-light irradiation. J Photochem Photobiol, A 333:92–104.  https://doi.org/10.1016/j.jphotochem.2016.10.021 CrossRefGoogle Scholar
  4. Atchudan R, Edison TN, Chakradhar D, Karthik N, Perumal S, Lee YR (2018a) One-pot dual product synthesis of hierarchical Co3O4@N-rGO for supercapacitors, N-GDs for label-free detection of metal ion and bio-imaging applications. Ceram Int 44:2869–2883.  https://doi.org/10.1016/j.ceramint.2017.11.034 CrossRefGoogle Scholar
  5. Atchudan R, Edison TN, Perumal S, Karthik N, Karthikeyan D, Shanmugam M, Lee YR (2018b) Concurrent synthesis of nitrogen-doped carbon dots for cell imaging and ZnO@nitrogen-doped carbon sheets for photocatalytic degradation of methylene blue. J Photochem Photobiol, A 350:75–85.  https://doi.org/10.1016/j.jphotochem.2017.09.038 CrossRefGoogle Scholar
  6. Blanton TN, Majumdar D (2013) Characterization of X-ray irradiated graphene oxide coatings using X-ray diffraction, X-ray photoelectron spectroscopy, and atomic force microscopy. Powder Diffr 28:68–71.  https://doi.org/10.1017/S0885715613000109 CrossRefGoogle Scholar
  7. Bonfoco E, Krainc D, Ankarcrona M, Nicotera P, Lipton SA (1995) Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci 92:7162–7166.  https://doi.org/10.1073/pnas.92.16.7162 CrossRefGoogle Scholar
  8. Chen J, Du D, Yan F, Ju HX, Lian HZ (2005) Electrochemical antitumor drug sensitivity test for leukemia K562 cells at a carbon-nanotube-modified electrode. Chem Eur J 11:1467–1472.  https://doi.org/10.1002/chem.200400956 CrossRefGoogle Scholar
  9. Chen YL, Mei T, Chen Y, Wang JY, Li JH, Fu Y, Dai GC, Wang S, Xiong WL, Wang XB (2016) A sensitive porphyrin/reduced graphene oxide electrode for simultaneous detection of guanine and adenine. J Solid State Electrochem 20:2055–2062.  https://doi.org/10.1007/s10008-016-3214-7 CrossRefGoogle Scholar
  10. Denecker G, Vercammen D, Declercq W, Vandenabeele P (2001) Apoptotic and necrotic cell death induced by death domain receptors. Cell Mol Life Sci 58:356–370.  https://doi.org/10.1007/pl00000863 CrossRefGoogle Scholar
  11. Dong Z, Saikumar P, Weinberg JM, Venkatachalam MA (1997) Internucleosomal DNA cleavage triggered by plasma membrane damage during necrotic cell death. Am J Pathol 151:1205–1213.  https://doi.org/10.1007/s004280050125 Google Scholar
  12. Elmore S (2007) Apoptosis: a review of programmed cell death. Toxicol Pathol 35:495–516.  https://doi.org/10.1080/01926230701320337 CrossRefGoogle Scholar
  13. Geng DS, Hu YH, Li YL, Li RY, Sun XL (2012) One-pot solvothermal synthesis of doped graphene with the designed nitrogen type used as a Pt support for fuel cells. Electrochem Commun 22:65–68.  https://doi.org/10.1016/j.elecom.2012.05.033 CrossRefGoogle Scholar
  14. Giribabu K, Suresh R, Manigandan R, Kumar SP, Muthamizh S, Munusamy S, Narayanan V (2014) Preparation of nitrogen-doped reduced graphene oxide and its use in a glassy carbon electrode for sensing 4-nitrophenol at nanomolar levels. Microchim Acta 181:1863–1870.  https://doi.org/10.1007/s00604-014-1251-4 CrossRefGoogle Scholar
  15. Gong XY, Liu J, Baskaran S, Voise RD, Young JS (2000) Surfactant-assisted processing of carbon nanotube/polymer composites. Chem Mater 12:1049–1052.  https://doi.org/10.1021/cm9906396 CrossRefGoogle Scholar
  16. Guo HL, Su P, Kang X, Ning SK (2013) Synthesis and characterization of nitrogen-doped graphene hydrogels by hydrothermal route with urea as reducing-doping agents. J Mater Chem A 1:2248–2255.  https://doi.org/10.1039/C2TA00887D CrossRefGoogle Scholar
  17. Hamberg H, Zhang L (1995) Quantitative determination of 8-hydroxyguanine and guanine by isotope dilution mass spectrometry. Anal Biochem 229:336–344.  https://doi.org/10.1006/abio.1995.1422 CrossRefGoogle Scholar
  18. Heisler I, Keller J, Tauber R, Sutherland M, Fuchs H (2002) A colorimetric assay for the quantitation of free adenine applied to determine the enzymatic activity of ribosome-inactivating proteins. Anal Biochem 302:114–122.  https://doi.org/10.1006/abio.2001.5527 CrossRefGoogle Scholar
  19. Imagawa Y, Saitoh T, Tsujimoto Y (2016) Vital staining for cell death identifies Atg9a-dependent necrosis in developmental bone formation in mouse. Nat Commun 7:1–10.  https://doi.org/10.1038/ncomms13391 CrossRefGoogle Scholar
  20. Kitanaka C, Kuchino Y (1999) Caspase-independent programmed cell death with necrotic morphology. Cell Death Differ 6:508–515.  https://doi.org/10.1038/sj.cdd.4400526 CrossRefGoogle Scholar
  21. Kovtyukhova NI, Ollivier PJ, Martin BR, Mallouk TE, Chizhik SA, Buzaneva EV, Gorchinskiy AD (1999) Layer-by-layer assembly of ultrathin composite films from micron-sized graphite oxide sheets and polycations. Chem Mater 11:771–778.  https://doi.org/10.1021/cm981085u CrossRefGoogle Scholar
  22. Kumar NA, Nolan H, McEvoy N, Rezvani E, Doyle RL, Lyons ME, Duesberg GS (2013) Plasma-assisted simultaneous reduction and nitrogen doping of graphene oxide nanosheets. J Mater Chem A 1:4431.  https://doi.org/10.1039/c3ta10337d CrossRefGoogle Scholar
  23. Lemasters JJ, Nieminen A, Qian T, Trost LC, Elmore SP, Nishimura Y, Crowe RA, Cascio WE, Bradham CA, Brenner DA, Herman B (1998) The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy. Biochim Biophys Acta 1366:177–196.  https://doi.org/10.1016/S0005-2728(98)00112-1 CrossRefGoogle Scholar
  24. Li JH, Kuang DZ, Feng YL, Zhang FX, Xu ZF, Liu MQ, Wang DP (2013) Green synthesis of silver nanoparticles-graphene oxide nanocomposite and its application in electrochemical sensing of tryptophan. Biosens Bioelectron 42:198–206.  https://doi.org/10.1016/j.bios.2012.10.029 CrossRefGoogle Scholar
  25. Li HY, Wang XL, Yu ZY (2014) Electrochemical biosensor for sensitively simultaneous determination of dopamine, uric acid, guanine, and adenine based on poly-melamine and nano Ag hybridized film-modified electrode. J Solid State Electrochem 18:105–113.  https://doi.org/10.1007/s10008-013-2242-9 CrossRefGoogle Scholar
  26. Li CJ, Chen C, Liao KX (2015) A quantitative study of signal characteristics of non-contact pipeline magnetic testing. Insight Non-Destr Test Cond Monitor 57:324–330.  https://doi.org/10.1784/insi.2015.57.6.324 CrossRefGoogle Scholar
  27. Li HY, Wang XL, Wang ZX, Zhao W (2016) Simultaneous determination of guanine, adenine, thymine and cytosine with a simple electrochemical method. J Solid State Electrochem 20:2223–2230.  https://doi.org/10.1007/s10008-016-3227-2 CrossRefGoogle Scholar
  28. Liang X, Zhang XY, Wang FW, Xu M (2014) Simultaneous determination of guanine and adenine on CuO shuttle-like nanocrystals/poly(neutral red) film on glassy carbon electrode. J Solid State Electrochem 18:3453–3461.  https://doi.org/10.1007/s10008-014-2564-2 CrossRefGoogle Scholar
  29. Majno G, Joris I (1995) Apoptosis, oncosis, and necrosis. An overview of cell death. Am J Pathol 55:3–15.  https://doi.org/10.1097/00002093-199509020-00009 Google Scholar
  30. Muthuchamy N, Atchudan R, Edison TN, Perumal S, Lee YR (2018) High-performance glucose biosensor based on green synthesized zinc oxide nanoparticle embedded nitrogen-doped carbon sheet. J Electroanal Chem 816:195–204.  https://doi.org/10.1016/j.jelechem.2018.03.059 CrossRefGoogle Scholar
  31. Perumal S, Raji A, Cheong IW (2018) Interaction of zwitterionic and ionic monomers with graphene surfaces. Langmuir 34:6737–6747.  https://doi.org/10.1021/acs.langmuir.8b00975 CrossRefGoogle Scholar
  32. Shao YY, Zhang S, Engelhard MH, Li GS, Shao GC, Wang Y, Liu J, Aksay IA, Lin Y (2010) Nitrogen-doped graphene and its electrochemical applications. J Mater Chem 20:7491–7496.  https://doi.org/10.1039/c0jm00782j CrossRefGoogle Scholar
  33. Sheng ZH, Shao L, Chen JJ, Bao WJ, Wang FB, Xia XH (2011) Catalyst-free synthesis of nitrogen-doped graphene via thermal annealing graphite oxide with melamine and its excellent electrocatalysis. ACS Nano 5:4350–4358.  https://doi.org/10.1021/nn103584t CrossRefGoogle Scholar
  34. Steel AB, Herne TM, Tarlov MJ (1998) Electrochemical quantitation of DNA immobilized on gold. Anal Chem 70:4670–4677.  https://doi.org/10.1021/ac980037q CrossRefGoogle Scholar
  35. Sun W, Li YZ, Duan YY, Jiao K (2008) Direct electrocatalytic oxidation of adenine and guanine on carbon ionic liquid electrode and the simultaneous determination. Biosens Bioelectron 24:988–993.  https://doi.org/10.1016/j.bios.2008.07.068 CrossRefGoogle Scholar
  36. Thangaraj R, Kumar AS (2013) Simultaneous detection of guanine and adenine in DNA and meat samples using graphitized mesoporous carbon modified electrode. J Solid State Electrochem 17:583–590.  https://doi.org/10.1007/s10008-012-1895-0 CrossRefGoogle Scholar
  37. Thanh TD, Balamurugan J, Lee SH, Lim NH, Lee JH (2016) Effective seed-assisted synthesis of gold nanoparticles anchored nitrogen-doped graphene for electrochemical detection of glucose and dopamine. Biosens Bioelectron 81:259–267.  https://doi.org/10.1016/j.bios.2016.02.070 CrossRefGoogle Scholar
  38. Usachov D, Vilkov O, Gruneis A, Haberer D, Fedorov A, Adamchuk VK, Preobrajenski AB, Dudin P, Barinov A, Oehzelt M, Laubschat C, Vyalik DV (2011) Nitrogen-doped graphene: efficient growth, structure, and electronic properties. Nano Lett 11:5401–5407.  https://doi.org/10.1021/nl2031037 CrossRefGoogle Scholar
  39. Wang XR, Li XL, Zhang L, Yoon Y, Weber PK, Wang HL, Guo J, Dai HL (2009) N-doping of graphene through electrothermal reactions with ammonia. Science 324:768–771.  https://doi.org/10.1126/science.1170335 CrossRefGoogle Scholar
  40. Wang HB, Maiyalagan T, Wang X (2012) Review on recent progress in nitrogen-doped graphene: synthesis, characterization, and its potential applications. ACS Catal 2:781–794.  https://doi.org/10.1021/cs200652y CrossRefGoogle Scholar
  41. Wang HB, Zhang HD, Xu LL, Gan T, Huang KJ, Liu YM (2014) Electrochemical biosensor for simultaneous determination of guanine and adenine based on dopamine-melanin colloidal nanospheres-graphene composites. J Solid State Electrochem 18:2435–2442.  https://doi.org/10.1007/s10008-014-2494-z CrossRefGoogle Scholar
  42. Wei DC, Liu YQ, Wang Y, Zhang HL, Huang LP, Yu G (2009) Synthesis of N-Doped graphene by chemical vapor deposition and its electrical properties. Nano Lett 9:1752–1758.  https://doi.org/10.1021/nl803279t CrossRefGoogle Scholar
  43. Wen ZH, Wang XC, Mao S, Bo Z, Kim H, Cui SM, Lu GH, Feng XL, Chen JH (2012) Crumpled nitrogen-doped graphene nanosheets with ultrahigh pore volume for high-performance supercapacitor. Adv Mater 24:5610–5616.  https://doi.org/10.1002/adma.201201920 CrossRefGoogle Scholar
  44. Yang FQ, Guan J, Li SP (2007) Fast simultaneous determination of 14 nucleosides and nucleobases in cultured Cordyceps using ultra-performance liquid chromatography. Talanta 73:269–273.  https://doi.org/10.1016/j.talanta.2007.03.034 CrossRefGoogle Scholar

Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical EngineeringNorthwest Normal UniversityLanzhouPeople’s Republic of China

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