Comparison of two morphologically different fungal biomass types for experimental separation of labile aluminium species using atomic spectrometry methods
This paper reflects some of the recent attempts to apply microbial biomass in analytical procedures, and thus investigates prospects of fungal biomass in experimental separation of labile Al species using flame atomic absorption spectrometry and inductively coupled plasma optical emission spectrometry for their quantification. The three strains of common microscopic filamentous fungi (Neosartorya fischeri, Aspergillus niger and Aspergillus clavatus) were cultivated under dynamic and static conditions to collect morphologically distinguished biomass types to compare their biosorptive uptake of Al. These data, along with 30-day bioaccumulation of Al, were then compared to the soluble fraction of labile positively charged monomeric Al species determined by 8-hydroxyquinoline method. Our results indicate that the labile Al species separation method by biomass was successful in case of 30-day cultivation where 65–109% of Al labile species were bioaccumulated by fungi. However, application of this long-term biological approach has several disadvantages compared to biosorption which, on the other hand, was less successful in separation of labile species with only up to 33% efficiency. Nevertheless, our results indicate that under certain conditions, there is a potential for the microscopic filamentous fungi being applied as a viable bioanalytical tool for the operationally and/or functionally defined fractionation of Al.
KeywordsLabile aluminium Soil microscopic filamentous fungi Biosorption Bioaccumulation Fractionation
The work was supported by the Scientific Grant Agency of the Slovak Republic Ministry of Education and the Slovak Academy of Sciences under contract nos. VEGA 1/0153/17, 1/0164/17, 1/0146/18, 1/0186/20 and 1/0192/20, and by Slovak Research and Development Agency under contract no. SK-KR-18-0003.
Compliance with ethical standards
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
- Boriova K, Urik M, Matus P (2015) Biosorption, bioaccumulation and biovolatilization of potentially toxic elements by microorganisms. Chem Listy 109:109–112Google Scholar
- Bradova M, Tejnecky V, Boruvka L, Nemecek K, Ash C, Sebek O, Svoboda M, Zenahlikova J, Drabek O (2015) The variations of aluminium species in mountainous forest soils and its implications to soil acidification. Environ Sci Poll Res 22:16676–16687. https://doi.org/10.1007/s11356-015-4855-2 CrossRefGoogle Scholar
- Dlapa P, Kubova J, Matus P, Stresko V (2002) Chemical and physical aluminium speciation in soil solutions. Fresenius Environ Bull 11:626–630Google Scholar
- Frankowski M, Ziola-Frankowska A, Kurzyca I, Novotny K, Vaculovic T, Kanicky V, Siepak M, Siepak J (2011) Determination of aluminium in groundwater samples by GF-AAS, ICP-AES, ICP-MS and modelling of inorganic aluminium complexes. Environ Monit Assess 182:71–84. https://doi.org/10.1007/s10661-010-1859-8 CrossRefGoogle Scholar
- Hlodak M, Matus P, Urik M, Korenkova L, Mikusova P, Senila M, Divis P (2015) Evaluation of Various Inorganic and Biological Extraction Techniques Suitability for Soil Mercury Phytoavailable Fraction Assessment. Water Air Soil Pollut 226:198. https://doi.org/10.1007/s11270-015-2458-7 CrossRefGoogle Scholar
- Matus P, Kubova J (2002) Speciation of aluminium in waters and soil solutions. Chem Listy 96:174–181Google Scholar
- Matus P, Kubova J (2008) Recent developments in the determination, fractionation and speciation analysis of aluminium by spectrochemical analytical methods and computer modelling. In: Dubois AN (ed) Soil contamination: new research. Nova Science, New York, pp 43–72Google Scholar
- Matus P, Kubova J, Stresko V (2003) Utilization of chelating ion exchange for aluminium speciation. Chem Pap 57:176–178Google Scholar
- Simonovicova A, Krakova L, Pauditsova E, Pangallo D (2019) Occurrence and diversity of cultivable autochthonous microscopic fungi in substrates of old environmental loads from mining activities in Slovakia. Ecotoxicol Environ Saf 172:194–202. https://doi.org/10.1016/j.ecoenv.2019.01.064 CrossRefGoogle Scholar
- Urik M, Matus P, Korenkova L (2018a) Mobilization and immobilization of potentially toxic metals and metalloids by filamentous fungi. In: Taylor JC (ed) Advances in chemistry research, vol 42. Nova Science, New York, pp 1–66Google Scholar