Assay of tipranavir capsules by a simple stability-indicating LC–UV method and cytotoxicity study of degraded samples

  • Leila P. Somavilla
  • Matheus W. Lago
  • Patrícia O. Barth
  • Daniele R. Nogueira-Librelotto
  • Clarice M. B. Rolim
  • Andréa I. H. AdamsEmail author
Original Paper


Tipranavir (TPV) is a protease inhibitor (PI) specially recommended for treatment-experienced patients who are resistant to other PI drugs. It was approved by FDA in 2005, but, until now, it has not been included in any official compendia. The objective of this study is to develop and validate a simple LC–UV method to assay TPV capsules, and to study the cytotoxicity of TPV and degraded samples over the cell viability. The optimized conditions were C8 Phenomenex® column (Luna®, 150 mm × 4.6 mm, 5 µm); mobile phase composed by methanol, acetonitrile, and acidified water pH 3.5 (40:31:29); flow rate 1.0 mL min−1 and detection at 254 nm. The cytotoxic effects of non-degraded and degraded TPV samples were evaluated in 3T3 cells by means of MTT viability assay. The method was linear in the range of 10–100 µg mL−1 (r = 0.9999) and specific, even in the presence of degradation products and impurities. The method showed suitable accuracy (mean recovery 100.02%), precision (RSD < 1.10%), and a two-level full factorial design indicated the robustness of the method. The degraded samples exhibited cytotoxicity patterns similar to TPV under controlled conditions. The method developed is appropriate for quality control of TPV capsules and stability studies.


Tipranavir Cytotoxicity Factorial design Stability Validation 



This research was supported by Project 459626/2014-0 of the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil). Cristalia (Brazil) donated the tipranavir reference substance and Hospital Universitário de Santa Maria (HUSM) provided the samples. Leila Paula Somavilla and Matheus Wagner Lago would like to thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001 (Brazil) for the Master’s fellowship.


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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.Programa de Pós-Graduação em Ciências FarmacêuticasUniversidade Federal de Santa MariaSanta MariaBrazil
  2. 2.Departamento de Farmácia IndustrialUniversidade Federal de Santa MariaSanta MariaBrazil

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