Validated micellar electrokinetic capillary chromatography (MECC) method for determination of 5-hydroxymethylfurfural in honey and comparison with HPLC

  • Tufan Güray
  • Neşe Yılmaz Tuncel
  • Muzaffer Tunçel
  • Ulku Dilek UysalEmail author
Original Paper


A simple and validated micellar electrokinetic chromatographic method is described for the determination of 5-hydroxymethylfurfural (HMF). The method was applied to fresh and processed (heated at 65 °C, 85 °C and 105 °C for 5 h and 20 h) multifloral and honeydew honeys. Optimum conditions were: 10 mmol L−1 Tris buffer consisting 110 mmol L−1 SDS and 10% (v/v) MeOH at pH 9.10, 20 kV of applied potential, 3 s of injection time at 5 × 103 N m−2, 280 nm of wavelength and 25 °C of fixed temperature. Mean electrophoretic mobility (m2 s−1 V−1) of HMF and methyl paraben (IS) were − 5.28 × 10−5 (RSD of 0.745%) and − 2.52 × 10−4 (RSD of 0.944%), respectively. The calibration curve was linear in the range of 0.68 µg mL−1 (5.40 × 10−6 mol L−1) and 4.05 µg mL−1 (3.21 × 10−5 mol L−1) with R = 0.9990 for inter-day precision. LOD and LOQ values were 0.007 µg mL−1 (5.48 × 10−8 mol L−1) and 0.023 µg mL−1 (1.83 × 10−7 mol L−1), respectively, as inter-day precision. The accuracy was very high with recovery values between 94.07 and 97.16%. The developed method was fully validated according to ICH guidelines. The results were compared with those of HPLC method adapted from AOAC method no: 980.23.


5-Hydroxymethylfurfural Honey HMF Capillary electrophoresis HPLC 



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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2019

Authors and Affiliations

  • Tufan Güray
    • 1
  • Neşe Yılmaz Tuncel
    • 2
  • Muzaffer Tunçel
    • 3
  • Ulku Dilek Uysal
    • 4
    Email author
  1. 1.Department of Chemistry, Faculty of Arts and ScienceEskisehir Osmangazi UniversityEskisehirTurkey
  2. 2.Department of Food Technology, School of Applied Sciences of ÇanakkaleOnsekiz Mart UniversityÇanakkaleTurkey
  3. 3.EskişehirTurkey
  4. 4.Department of Chemistry, Faculty of ScienceEskisehir Technical UniversityEskişehirTurkey

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