A colorimetric microfluidic sensor made by a simple instrumental-free prototyping process for sensitive quantitation of copper
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A simple, cost-effective, instrumental-free prototyping process has been developed for fabricating flexible, multilayer colorimetric microfluidic sensor. A hand-hold punch was used to make microfluidic sensor pattern with no use of any expensive instruments (laser cutter, cutting plotter, screen printer, wax printer, etc.). Colorimetric analysis was carried out using a smartphone camera as a reader. Sensitive quantitation of copper has been demonstrated on the developed sensor under the optimal parameters. In the presence of copper ion, the Blue channel color values decreased with increasing the Cu2+ concentration. The Blue channel color intensity was linear with the concentration of Cu2+ in the range from 0 to 30 mg/L with a detection limit of 0.096 mg/L (3σ). The developed microfluidic sensor possesses good selectivity, satisfying reproducibility and high recoveries in tap water. Furthermore, through changing hole punch with different hole shape and hole numbers, it is extremely easy to produce microfluidic sensors with different design in quantity at low cost. What’s more, the developed sensor could be easily extended to detect other single analyte or multiple analytes, showing promising practical applications in environmental analysis.
KeywordsMicrofluidic Colorimetry Sensor Copper
This work was financially supported by Natural Science Foundation of Hunan Province (Grant No. 2018JJ3500), Research Foundation for PhD of Xiangtan University (Grant No. KZ08042) and National Science and Technology Major Project (Grant No. 2016ZX05040-002).
Compliance with ethical standards
Conflict of interest
There are no conflicts to declare.
- Antunes GA, Santos HSD, Silva YPD, Silva MM, Piatnicki CM, Samios D (2017) Determination of iron, copper, zinc, aluminum, and chromium in biodiesel by flame atomic absorption spectrometry using a microemulsion preparation method. Energy Fuel 31:2944–2950. https://doi.org/10.1021/acs.energyfuels.6b03360 CrossRefGoogle Scholar
- Chaiyo S, Siangproh W, Apilux A, Chailapakul O (2015) Highly selective and sensitive paper-based colorimetric sensor using thiosulfate catalytic etching of silver nanoplates for trace determination of copper ions. Anal Chim Acta 866:75–83. https://doi.org/10.1016/j.aca.2015.01.042 CrossRefGoogle Scholar
- Chen H, Jin J, Wang Y (1997) Flow injection on-line coprecipitation-preconcentration system using copper(II) diethyldithiocarbamate as carrier for flame atomic absorption spectrometric determination of cadmium, lead and nickel in environmental samples. Anal Chim Acta 353:181–188. https://doi.org/10.1016/S0003-2670(97)87776-8 CrossRefGoogle Scholar
- Cunningham JC, DeGregory PR, Crooks RM (2016) New functionalities for paper-based sensors lead to simplified user operation, lower limits of detection, and new applications. Annu Rev Anal Chem 9:183–202. https://doi.org/10.1146/annurev-anchem-071015-041605 CrossRefGoogle Scholar
- Fan C, Lv X, Liu F, Feng L, Liu M, Cai Y, Liu H, Wang J, Yang Y, Wang H (2018) Silver nanoclusters encapsulated into metal-organic frameworks with enhanced fluorescence and specific ion accumulation toward the microdot array-based fluorimetric analysis of copper in blood. ACS Sens 3:441–450. https://doi.org/10.1021/acssensors.7b00874 CrossRefGoogle Scholar
- Ferreira SL, Queiroz AS, Fernandes MS, Santos HCD (2002) Application of factorial designs and Doehlert matrix in optimization of experimental variables associated with the preconcentration and determination of vanadium and copper in seawater by inductively coupled plasma optical emission spectrometry. Spectrochim Acta B 57:1939–1950. https://doi.org/10.1016/S0584-8547(02)00160-X CrossRefGoogle Scholar
- Jin L, Davis MR, Hu P, Baillie TA (1994) Identification of novel glutathione conjugates of disulfiram and diethyldithiocarbamate in rat bile by liquid chromatography-tandem mass spectrometry. Evidence for metabolic activation of disulfiram in vivo. Chem Res Toxicol 7:526–533. https://doi.org/10.1021/tx00040a008 CrossRefGoogle Scholar
- Marczenko Z, Balcerzak M (2000) Separation, preconcentration and spectrophotometry in inorganic analysis. Elsevier, New YorkGoogle Scholar
- Nguyen TT, Yoon WJ, Lee NY, Ju H (2017) Integration of a microfluidic polymerase chain reaction device and surface plasmon resonance fiber sensor into an inline all-in-one platform for pathogenic bacteria detection. Sens Actuator B Chem 242:1–8. https://doi.org/10.1016/j.snb.2016.10.137 CrossRefGoogle Scholar
- Sadollahkhani A, Hatamie A, Nur O, Willander M, Zargar B, Kazeminezhad I (2014) Colorimetric disposable paper coated with ZnO@ZnS core-shell nanoparticles for detection of copper ions in aqueous solutions. ACS Appl Mater Interfaces 6:17694–17701. https://doi.org/10.1021/am505480y CrossRefGoogle Scholar
- Wu P, Gao Y, Cheng G, Yang W, Lv Y, Hou X (2008) Selective determination of trace amounts of silver in complicated matrices by displacement-cloud point extraction coupled with thermospray flame furnace atomic absorption spectrometry. J Anal Atom Spectrom 23:752–757. https://doi.org/10.1039/B719579F CrossRefGoogle Scholar
- Wu YY, Boonloed A, Sleszynski N, Koesdjojo M, Armstrong C, Bracha S, Remcho VT (2015) Clinical chemistry measurements with commercially available test slides on a smartphone platform: colorimetric determination of glucose and urea. Clin Chim Acta 448:133–138. https://doi.org/10.1016/j.cca.2015.05.020 CrossRefGoogle Scholar
- Yan XP, Li Y, Jiang Y (2003) Selective measurement of ultratrace methylmercury in fish by flow injection on-line microcolumn displacement sorption preconcentration and separation coupled with electrothermal atomic absorption spectrometry. Anal Chem 75:2251–2255. https://doi.org/10.1021/ac026415f CrossRefGoogle Scholar