Attenuation of hydrogen peroxide-induced oxidative stress in SH-SY5Y cells by three flavonoids from Acer okamotoanum
- 23 Downloads
The flavonoids quercitrin, isoquercitrin, and afzelin were isolated from the ethyl acetate fraction of Acer okamotoanum. The effectiveness of these flavonoids in protecting human cells was investigated using SH-SY5Y neuronal cells. Oxidative stress was induced by hydrogen peroxide (H2O2) and cells treated with flavonoids had an increased viability as compared with untreated cells. The flavonoid-treated cells showed significantly less reactive oxygen species production and lactase dehydrogenase release than the untreated cells. Especially, quercitrin and isoquercitrin showed the strongest protective effects against oxidative stress among several tested flavonoids as determined by the results for cell viability, reactive oxygen species (ROS) production, and lactate dehydrogenase release. Furthermore, we measured the expression of inflammation- and apoptosis-related proteins. These flavonoids attenuated inflammation by downregulating the expression of cyclooxygenase-2, inducible nitric oxide synthase, and apoptotic signaling via inhibiting caspase activation. The present results suggest that three flavonoids of A. okamotoanum protect against cellular oxidative stress possibly through regulating inflammation, apoptosis, and ROS-scavenging.
KeywordsAcer okamotoanum Afzelin Isoquercitrin Oxidative stress Quercitrin
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A01058868), Republic of Korea. This research was supported by Global PH.D Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016_H1A2A1906940).
- Jin L, Han JG, Ha JH, Jeong HS, Kwon MC, Jeong MH, Lee HJ, Kang HY, Choi DH, Lee HY (2008) Comparison of antioxidant and glutathione S-transferase activities of extracts from Acer mono and A. okamotoanum. Korean J Med Crop Sci 16:427–433Google Scholar
- Lee S, Park HS, Notsu Y, Ban HS, Kim YP, Ishihara K, Hirasawa N, Jung SH, Lee YS, Lim SS, Park EH, Shin KH, Seyama T, Hong J, Ohuchi K (2008) Effects of hyperin, isoquercitrin and quercetin on lipopolysaccharide-induced nitrite production in rat peritoneal macrophages. Phytother Res 22:1552–1556CrossRefGoogle Scholar
- Li X, Mai W, Chen D (2014) Chemical study on protective effect against hydroxyl-induced DNA damage and antioxidant mechanism of myricitrin. J Chin Chem Soc 61:390–393Google Scholar
- Li L, Zhang XH, Liu GR, Liu C, Dong YM (2016a) Isoquercitrin suppresses the expression of histamine and pro-inflammatory cytokines by inhibiting the activation of MAP Kinases and NF-κB in human KU812 cells. Chin J Nat Med 14:407–412Google Scholar
- Qadir SA, Kim CH, Kwon MC, Lee HJ, Kang HY, Choi DH, Lee HY (2007) Comparison of anticancer and immuno-modulatory activities in the different parts of the Acer mono Max. and Acer okamotoanum. Korean J Med Crop Sci 15:405–410Google Scholar
- Vellosa JC, Regasini LO, Belló C, Schemberger JA, Khalil NM, de Araújo Morandim-Giannetti A, da Silva Bolzani V, Brunetti IL, de Faria Oliveira OM (2015) Preliminary in vitro and ex vivo evaluation of afzelin, kaempferitrin and pterogynoside action over free radicals and reactive oxygen species. Arch Pharm Res 38:1168–1177CrossRefGoogle Scholar