Development of silver nanoparticles-loaded CMC hydrogel using bamboo as a raw material for special medical applications
- 50 Downloads
The present investigation focuses on the synthesis of silver nanoparticles (Ag-NPs)-loaded hydrogel from synthetic and lab-made carboxymethyl cellulose (CMC) using bamboo as a raw material. The Ag-NPs were made by in situ process by the reaction of CMC and epichlorohydrin in alkaline medium containing silver nitrate. CMC acts as a stabilizer and reducing agent in the formation of nanoparticles. The formation of Ag-NPs was confirmed by UV–Vis spectrophotometer, as an absorption peak around 425 nm was observed. The Ag-NPs antibacterial activity against Escherichia coli (ATCC 433), Bacillus subtilis (ATCC 1688) was calculated by agar disk diffusion technique method. The characterization of the Ag-NPs was done by FTIR, SEM, TEM, particle size analyzer and EDX. Ag-NPs obtained from lab-made CMC displayed a higher antibacterial action towards Gram-negative (Escherichia coli) bacteria as well as Gram-Positive bacteria (Bacillus subtilis) as compared to synthetic CMC obtained from market.
KeywordsCarboxymethyl cellulose Silver nanoparticles (Ag-NPs) Silver nitrate Antibacterial activity Hydrogel
We greatly acknowledge the support of CIR, MNNIT Allahabad and department of physics, Allahabad University for permitting us to use analytical instruments.
- Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N (2010) Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B 76:50–56. https://doi.org/10.1016/j.colsurfb.2009.10.008 CrossRefGoogle Scholar
- Lustosa AKMF, de Jesus Oliveira AC, Quelemes PV, Placido A, da Silva FV, Oliveira IS, de Almeida MP, Amorim ADGN, Delerue-Matos C, Eaton P (2017) In situ synthesis of silver nanoparticles in a hydrogel of carboxymethyl cellulose with phthalated-cashew gum as a promising antibacterial and healing agent. Int J Mol Sci 18:1–15. https://doi.org/10.3390/ijms18112399 CrossRefGoogle Scholar
- Pedroza-Toscano MA, López-Cuenca S, Rabelero-Velasco M, Moreno-Medrano ED, Mendizabal-Ruiz AP, Salazar-Pena R (2017) Silver nanoparticles obtained by semicontinuous chemical reduction using carboxymethyl cellulose as a stabilizing agent and its antibacterial capacity. J Nanomater. https://doi.org/10.1155/2017/1390180 Google Scholar
- Vimala K, Murali Mohan Y, Samba Sivudua K, Varaprasada K, Ravindra S, Narayana Reddya N, Padma Y, Sreedhar B, MohanaRajun K (2010) Fabrication of porous chitosan films impregnated with silver nanoparticles: a facile approach for superior antibacterial application. Colloids Surf B 76:248–258. https://doi.org/10.1016/j.colsurfb.2009.10.044 CrossRefGoogle Scholar