Investigation of membrane bioreactor for in situ product removal based on silicone rubber membrane module
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In many biotransformation productions performed in ordinary batch or fed-batch bioreactors, product inhibition of a production strain strongly decreases the yield and effectivity of the process. A way to overcome this effect is to apply extractive biotransformation, which means to continually remove the product from the fermentation broth. Nowadays, application of a membrane bioreactor with an immersed capillary membrane module is used as a promising solution for this case. In this work, we propose the membrane bioreactor for extractive bioproduction of chemical specialties consisting of a 3 L mixed tank bioreactor with an immersed extractive tubular membrane module. As the membrane material, silicone rubber tubes were chosen as it shows many advantages compared to other materials. As the model solute for the extraction, 2-phenylethanol (rose aroma) was chosen due to its strong inhibition effect on the production strain (Saccharomyces cerevisiae). The solute partition coefficient in the extraction system containing solute, water and silicone rubber was measured as well as the solute diffusion coefficient for the silicone rubber membrane. Three different membrane modules made of silicone rubber tubes were manufactured and tested in series of extraction experiments performed in the membrane bioreactor at different operation conditions including different biomass concentration, stirring rate, and aeration rate. Experimental data were compared with the prediction of mathematical model programmed in MATLAB with good accuracy.
KeywordsExtractive biotransformation Immersed module Membrane extraction Silicone rubber 2-Phenylethanol
This work was supported by the Slovak Scientific Agency, Grant No. VEGA 1/0687/16, and the Slovak Research and Development Agency under the contract No. APVV-16-0111. This article was created with the support of the Ministry of Education, Science, Research and Sport of the Slovak Republic within the Research and Development Operational Programme for the project “University Science Park of STU Bratislava”, ITMS 26240220084, co-funded by the European Regional Development Fund.
- Doig SD, Boam AT, Leak DI, Livingston AG, Stuckey DC (1998) A membrane bioreactor for biotransformation of hydrophobic molecules. Biotechnol Bioeng 58:587–594. https://doi.org/10.1002/(SICI)1097-0290(19980620)58:6%3c587:AID-BIT4%3e3.0.CO;2-E CrossRefGoogle Scholar
- Molinari F, Gandolfi R, Aragozzini F, Leon R, Prazeres DMF (1999) Biotransformations in two-liquid-phase systems: production of phenylacetaldehyde by oxidation of 2-phenylethanol with acetic acid bacteria. Enzyme Microb Technol 25:729–735. https://doi.org/10.1016/S0141-0229(99)00107-6 CrossRefGoogle Scholar
- Reid RC, Prausnitz JM, Poling HE (1987) The properties of gases and liquids. McGraw-Hill, New YorkGoogle Scholar
- Xiao M, Zhou JT, Zhang AL, Yin GJ (2009) Study of mass transfer characteristics of membrane extraction of phenol aqueous solution using non-porous silicone rubber membrane. J Dalian Univ Tech 49:333–339Google Scholar
- Xiao M, Hu X, Li S (2013) Mass transfer process in nonporous tubular membrane for extraction of phenol with caustic stripping solution C3. Adv Mater Res 610–613:1229–1232. https://doi.org/10.4028/www.scientific.net/AMR.610-613.1229 CrossRefGoogle Scholar