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Obesity Surgery

, Volume 29, Issue 2, pp 593–600 | Cite as

Technical Feasibility of a Murine Model of Sleeve Gastrectomy with Ileal Transposition

  • Lee D. Ying
  • Gregory A. Breuer
  • Matthew O. Hubbard
  • Geoffrey S. Nadzam
  • John Hwa
  • Kathleen A. MartinEmail author
Original Contributions
  • 80 Downloads

Abstract

Background

Sleeve gastrectomy with ileal transposition has been shown to be superior to sleeve gastrectomy alone for promoting weight loss in rat and porcine models. The absence of a mouse model for this procedure has impeded efforts to understand the molecular physiology underlying its efficacy. This study demonstrates the long-term survivability of sleeve gastrectomy with ileal transposition in mice.

Materials and Methods

In this study of technical feasibility, a sleeve gastrectomy with ileal transposition (SGIT), sleeve gastrectomy (SG), or sham surgery (SH) was performed on 7- to 8-week-old C57Bl/6J mice (n = 8 for each). To evaluate long-term survivability, mice were placed on an obesogenic diet and weighed weekly for 10 weeks. The intestinal identity of the transposed segment was assessed with gene expression analysis of duodenal-, jejunal-, and ileal-specific hormones using quantitative polymerase chain reaction.

Results

Overall, SGIT better prevented weight gain than the SG or sham procedures (10-week post-operative weight: SH 45.3 ± 1.0 g, SG 41.25 ± 1.6 g, SGIT 35.4 ± 0.8 g). Gene expression pattern analysis of three markers of intestinal identity (gastrin, cholecystokinin, and peptide YY) suggests that the ileal identity of the transposed segment is maintained 10 weeks after transposition.

Conclusions

We demonstrate for the first time a reproducible mouse model of sleeve gastrectomy with ileal transposition. Future studies utilizing this model will expand our understanding of the molecular pathways through which the hindgut regulates satiety.

Keywords

Mouse metabolic surgery Sleeve gastrectomy with ileal transposition 

Notes

Author Contributions

L.D.Y. conceived of, performed, and interpreted the research, and wrote and edited the manuscript.

G.B., M.O.H., G.S.N., J.H., and K.A.M. conceived of and interpreted the research, and wrote and edited the manuscript.

Funding

Lee D. Ying is supported by the NIH NIDDK (1F30DK112569-01).

Kathleen A. Martin is supported by the NIH NHLBI (HL091013, HL118430, RHL119529).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

All applicable institutional and/or national guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Lee D. Ying
    • 1
    • 2
  • Gregory A. Breuer
    • 1
  • Matthew O. Hubbard
    • 1
    • 3
  • Geoffrey S. Nadzam
    • 1
    • 3
  • John Hwa
    • 1
    • 2
  • Kathleen A. Martin
    • 1
    • 2
    Email author
  1. 1.Yale University School of MedicineNew HavenUSA
  2. 2.Yale Cardiovascular Research CenterNew HavenUSA
  3. 3.Department of Gastrointestinal SurgeryYale New Haven HealthNew HavenUSA

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