Tissue-Specific Phenotype and Activation of iNKT Cells in Morbidly Obese Subjects: Interaction with Adipocytes and Effect of Bariatric Surgery

  • Soledad López
  • Sara García-Serrano
  • Carolina Gutierrez-Repiso
  • Francisca Rodríguez-Pacheco
  • Ailec Ho-Plagaro
  • Concepción Santiago-Fernandez
  • Gonzalo Alba
  • Marta Cejudo-Guillen
  • Alberto Rodríguez-Cañete
  • Sergio Valdes
  • Lourdes Garrido-Sanchez
  • David Pozo
  • Eduardo García-Fuentes
Original Contributions

Abstract

Background

The immune response of visceral adipose tissue (VAT) in obesity, in particular the role of invariant natural killer T (iNKT) cells, has not yet been fully elucidated.

Objective

To characterize iNKT cells and its activation status in VAT and peripheral blood mononuclear cells (PBMC) in morbidly obese subjects (MO), and to analyze their association with metabolic parameters.

Subjects and Methods

Twenty non-obese and 20 MO subjects underwent Roux-en-Y gastric bypass (RYGB) and were studied before and 6 months after RYGB. VAT and PBMC were obtained.

Results

A decrease in VAT iNKT cells from MO was found, however, not in PBMC. Visceral adipocytes from MO presented increased CD1d expression (p = 0.032). MO presented an increase in early activated CD69+ iNKT cells in PBMC before RYGB (p < 0.001), but not after RYGB nor in VAT, and an increase in later activated CD25+ iNKT in VAT (p = 0.046), without differences in PBMC. The co-expression of early and later markers (CD69+CD25+) in iNKT cells was increased in MO in VAT (p = 0.050) and PBMC (p = 0.006), decreasing after RYGB (p = 0.050). CD69+ iNKT and CD69+CD25+ iNKT cells in PBMC after RYGB correlated negatively with glucose, insulin, and insulin resistance levels.

Conclusions

There is a tissue-specific phenotype and activation of iNKT cells in VAT in morbid obesity, which could be involved in VAT immunometabolism dysregulation. Also, the increase in CD1d expression could be to offset the lack of VAT iNKT cells.

Keywords

Natural killer T cells Inflammation Obesity Adipose tissue 

Notes

Acknowledgments

CIBERObn is an ISCIII project. We gratefully acknowledge the Imaging platform of the Institute of Biomedical Research in Malaga (IBIMA), as well as the technical assistance from Gomez-Conde, AI.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no competing interests.

Supplementary material

11695_2018_3215_MOESM1_ESM.pptx (658 kb)
ESM 1 (PPTX 657 kb)
11695_2018_3215_MOESM2_ESM.docx (40 kb)
ESM 2 (DOCX 40 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Soledad López
    • 1
    • 2
    • 3
  • Sara García-Serrano
    • 4
    • 5
  • Carolina Gutierrez-Repiso
    • 6
  • Francisca Rodríguez-Pacheco
    • 4
  • Ailec Ho-Plagaro
    • 7
  • Concepción Santiago-Fernandez
    • 7
  • Gonzalo Alba
    • 1
  • Marta Cejudo-Guillen
    • 2
  • Alberto Rodríguez-Cañete
    • 8
  • Sergio Valdes
    • 5
    • 6
  • Lourdes Garrido-Sanchez
    • 4
    • 9
  • David Pozo
    • 1
    • 2
  • Eduardo García-Fuentes
    • 7
    • 9
    • 10
  1. 1.Department of Medical Biochemistry, Molecular Biology and ImmunologyUniversity of Seville Medical SchoolSevilleSpain
  2. 2.CABIMER-Andalusian Center for Molecular Biology and Regenerative Medicine (CSIC-University of Seville-UPO-Junta de Andalucia)SevilleSpain
  3. 3.Dpto. Bioquímica Médica, Biología Molecular e Inmunología, Facultad de MedicinaUniversidad de SevillaSevillaSpain
  4. 4.Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA)Hospital Regional UniversitarioMalagaSpain
  5. 5.CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM), Instituto de Salud Carlos IIIMalagaSpain
  6. 6.Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA)Hospital Universitario Virgen de la VictoriaMalagaSpain
  7. 7.Unidad de Gestión Clínica de Aparato Digestivo, Instituto de Investigación Biomédica de Málaga (IBIMA)Hospital Universitario Virgen de la VictoriaMalagaSpain
  8. 8.Unidad de Gestión Clínica de Cirugía General, Digestiva y TrasplantesHospital Regional UniversitarioMalagaSpain
  9. 9.CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos IIIMalagaSpain
  10. 10.Laboratorio de InvestigaciónHospital CivilMalagaSpain

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