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Optimization of green extraction of phytochemicals from red grape pomace by homogenizer assisted extraction

  • Vildan Eyiz
  • Ismail TontulEmail author
  • Selman Turker
Original Paper
  • 17 Downloads

Abstract

Grape pomace is a by-product that contains high amounts of phytochemicals such as phenolic acids, flavonoids, and anthocyanins. For the efficient extraction of these compounds, extraction optimization was done in the present study. Glycerol concentration, a cheap, non-toxic and abundant green solvent, and liquid–solid ratio were evaluated as the independent factor of the optimization and total phenolic content, total flavonoid content, free radical scavenging activity, total monomeric anthocyanin content, total proanthocyanidin content and ascorbic acid content was analyzed as responses. The result showed that glycerol concentration significantly affected all responses and increasing the concentration resulted in higher extraction of the phytochemicals. Additionally, liquid–solid ratio affected total phenolic content, total flavonoid content, total proanthocyanidin content and ascorbic acid content of the extracts. Glycerol concentration of 50% (w/v) and liquid–solid ratio of 22.4 was determined to be optimum condition to maximize all responses. The optimum conditions were experimentally validated. Overall, the study showed that homogenizer assisted extraction using glycerol as a green solvent is a good method for extraction of phytochemicals from food processing by-products.

Keywords

Anthocyanins Glycerol Green extraction Phenolics Response surface methodology 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    A. Apostolakis, S. Grigorakis, D.P. Makris, Optimisation and comparative kinetics study of polyphenol extraction from olive leaves (Olea europaea) using heated water/glycerol mixtures. Sep. Purif. Technol. 128, 89–95 (2014).  https://doi.org/10.1016/j.seppur.2014.03.010 CrossRefGoogle Scholar
  2. 2.
    G. Ruberto, A. Renda, C. Daquino, V. Amico, C. Spatafora, C. Tringali, N.D. Tommasi, Polyphenol constituents and antioxidant activity of grape pomace extracts from five Sicilian red grape cultivars. Food Chem. 100(1), 203–210 (2007).  https://doi.org/10.1016/j.foodchem.2005.09.041 CrossRefGoogle Scholar
  3. 3.
    Y. Lu, L. Yeap Foo, The polyphenol constituents of grape pomace. Food Chem. 65(1), 1–8 (1999).  https://doi.org/10.1016/S0308-8146(98)00245-3 CrossRefGoogle Scholar
  4. 4.
    J.M. Yu, M. Ahmedna, Functional components of grape pomace: their composition, biological properties and potential applications. Int. J. Food Sci. Technol. 48(2), 221–237 (2013).  https://doi.org/10.1111/j.1365-2621.2012.03197.x CrossRefGoogle Scholar
  5. 5.
    B. Karakashov, S. Grigorakis, S. Loupassaki, D.P. Makris, Optimisation of polyphenol extraction from Hypericum perforatum (St. John's Wort) using aqueous glycerol and response surface methodology. J. Appl. Res. Med. Aromat. Plants 2(1), 1–8 (2015). https://doi.org/10.1016/j.jarmap.2014.11.002 Google Scholar
  6. 6.
    P. Sigala, S. Grigorakis, D.P. Makris, Kinetics of ultrasound-assisted polyphenol extraction from spent filter coffee using aqueous glycerol. Chem. Eng. Commun. 203(3), 407–413 (2016).  https://doi.org/10.1080/00986445.2015.1004667 CrossRefGoogle Scholar
  7. 7.
    K. Philippi, N. Tsamandouras, S. Grigorakis, D.P. Makris, Ultrasound-assisted green extraction of eggplant peel (Solanum melongena) polyphenols using aqueous mixtures of glycerol and ethanol: optimisation and kinetics. Environ. Process. 3(2), 369–386 (2016).  https://doi.org/10.1007/s40710-016-0140-8 CrossRefGoogle Scholar
  8. 8.
    D.P. Makris, V. Passalidi, S. Kallithraka, I. Mourtzinos, Optimization of polyphenol extraction from red grape pomace using aqueous glycerol/tartaric acid mixtures and response surface methodology. Prep. Biochem. Biotechnol. 46(2), 176–182 (2016).  https://doi.org/10.1080/10826068.2015.1015562 CrossRefGoogle Scholar
  9. 9.
    A. Michail, P. Sigala, S. Grigorakis, D.P. Makris, Kinetics of ultrasound-assisted polyphenol extraction from spent filter coffee using aqueous glycerol. Chem. Eng. Commun. 203(3), 407–413 (2016).  https://doi.org/10.1080/00986445.2015.1004667 CrossRefGoogle Scholar
  10. 10.
    M. Bilgin, S. Şahin, Effects of geographical origin and extraction methods on total phenolic yield of olive tree (Olea europaea) leaves. J. Taiwan Inst. Chem. Eng. 44(1), 8–12 (2013).  https://doi.org/10.1016/j.jtice.2012.08.008 CrossRefGoogle Scholar
  11. 11.
    B. Baria, N. Upadhyay, A.K. Singh, R.K. Malhotra, Optimization of ‘green’ extraction of carotenoids from mango pulp using split plot design and its characterization. LWT 104, 186–194 (2019).  https://doi.org/10.1016/j.lwt.2019.01.044 CrossRefGoogle Scholar
  12. 12.
    G.A. Pereira, G. Molina, H.S. Arruda, G.M. Pastore, Optimizing the homogenizer-assisted extraction (HAE) of total phenolic compounds from banana peel. J. Food Process Eng. 40(3), e12438 (2017).  https://doi.org/10.1111/jfpe.12438 CrossRefGoogle Scholar
  13. 13.
    M. Bilgin, S. Sahin, M.U. Dramur, L.M. Sevgili, Obtaining scarlet sage (Salvia coccinea) extract through homogenizer- and ultrasound-assisted extraction methods. Chem. Eng. Commun. 200(9), 1197–1209 (2013).  https://doi.org/10.1080/00986445.2012.742434 CrossRefGoogle Scholar
  14. 14.
    G. Derringer, R. Suich, Simultaneous optimization of several response variables. J. Qual. Technol. 12(4), 214–219 (1980)CrossRefGoogle Scholar
  15. 15.
    C. Dincer, A. Topuz, H. Sahin-Nadeem, K.S. Ozdemir, I.B. Cam, I. Tontul, R.S. Gokturk, S.T. Ay, A comparative study on phenolic composition, antioxidant activity and essential oil content of wild and cultivated sage (Salvia fruticosa Miller) as influenced by storage. Ind. Crops Prod. 39, 170–176 (2012).  https://doi.org/10.1016/j.indcrop.2012.02.032 CrossRefGoogle Scholar
  16. 16.
    C.-H. Chang, H.-Y. Lin, C.-Y. Chang, Y.-C. Liu, Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes. J. Food Eng. 77(3), 478–485 (2006).  https://doi.org/10.1016/j.jfoodeng.2005.06.061 CrossRefGoogle Scholar
  17. 17.
    I. Tontul, A. Topuz, Effects of different drying methods on the physicochemical properties of pomegranate leather (pestil). LWT Journal 80, 294–303 (2017).  https://doi.org/10.1016/j.lwt.2017.02.035 CrossRefGoogle Scholar
  18. 18.
    T. Barros, L. Galego, P. Pires-Cabral, Monstera deliciosa fruit: physicochemical characterization and potential for distillate production. J. Food Meas. Charact. 12(4), 2874–2882 (2018).  https://doi.org/10.1007/s11694-018-9902-5 CrossRefGoogle Scholar
  19. 19.
    S. Blidi, M. Bikaki, S. Grigorakis, S. Loupassaki, D.P. Makris, A comparative evaluation of bio-solvents for the efficient extraction of polyphenolic phytochemicals: apple waste peels as a case study. Waste Biomass Valoriz. 6(6), 1125–1133 (2015)CrossRefGoogle Scholar
  20. 20.
    Q. Xu, Y. Shen, H. Wang, N. Zhang, S. Xu, L. Zhang, Application of response surface methodology to optimise extraction of flavonoids from Fructus sophorae. Food Chem. 138(4), 2122–2129 (2013).  https://doi.org/10.1016/j.foodchem.2012.11.099 CrossRefGoogle Scholar
  21. 21.
    S.I. Mussatto, L.F. Ballesteros, S. Martins, J.A. Teixeira, Extraction of antioxidant phenolic compounds from spent coffee grounds. Sep. Purif. Technol. 83, 173–179 (2011).  https://doi.org/10.1016/j.seppur.2011.09.036 CrossRefGoogle Scholar
  22. 22.
    W.H. Wong, W.X. Lee, R.N. Ramanan, L.H. Tee, K.W. Kong, C.M. Galanakis, J. Sun, K.N. Prasad, Two level half factorial design for the extraction of phenolics, flavonoids and antioxidants recovery from palm kernel by-product. Ind. Crops Prod. 63, 238–248 (2015).  https://doi.org/10.1016/j.indcrop.2014.09.049 CrossRefGoogle Scholar
  23. 23.
    L. Yang, Y.L. Cao, J.G. Jiang, Q.S. Lin, J. Chen, L. Zhu, Response surface optimization of ultrasound-assisted flavonoids extraction from the flower of Citrus aurantium L. var. amara. Engl. J Sep Sci 33(9), 1349–1355 (2010).  https://doi.org/10.1002/jssc.200900776 Google Scholar
  24. 24.
    P. Jing, M.M. Giusti, Effects of extraction conditions on improving the yield and quality of an anthocyanin-rich purple corn (Zea mays L.) color extract. J. Food Sci. 72(7), C363–368 (2007).  https://doi.org/10.1111/j.1750-3841.2007.00441.x CrossRefGoogle Scholar
  25. 25.
    G.-L. Liu, H.-H. Guo, Y.-M. Sun, Optimization of the extraction of anthocyanins from the fruit skin of Rhodomyrtus tomentosa (Ait) Hassk and identification of anthocyanins in the extract using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). Int. J. Mol. Sci. 13(5), 6292–6302 (2012)CrossRefGoogle Scholar
  26. 26.
    J.P. Maran, V. Sivakumar, K. Thirugnanasambandham, R. Sridhar, Extraction of natural anthocyanin and colors from pulp of jamun fruit. J. Food Sci. Technol. 52(6), 3617–3626 (2015).  https://doi.org/10.1007/s13197-014-1429-0 Google Scholar
  27. 27.
    H.-L. Jiang, J.-L. Yang, Y.-P. Shi, Optimization of ultrasonic cell grinder extraction of anthocyanins from blueberry using response surface methodology. Ultrason. Sonochem. 34, 325–331 (2017).  https://doi.org/10.1016/j.ultsonch.2016.06.003 CrossRefGoogle Scholar
  28. 28.
    Q.V. Vuong, S. Hirun, P.D. Roach, M.C. Bowyer, P.A. Phillips, C.J. Scarlett, Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts. J. Herb. Med. 3(3), 104–111 (2013).  https://doi.org/10.1016/j.hermed.2013.04.004 CrossRefGoogle Scholar
  29. 29.
    N.-Y. Kim, M.-K. Jang, D.-G. Lee, K.H. Yu, H. Jang, M. Kim, S.G. Kim, B.H. Yoo, S.-H. Lee, Comparison of methods for proanthocyanidin extraction from pine (Pinus densiflora) needles and biological activities of the extracts. Nutr. Res. Pract. 4(1), 16–22 (2010)CrossRefGoogle Scholar
  30. 30.
    D.J. Bhuyan, Q. Van Vuong, A.C. Chalmers, I.A. van Altena, M.C. Bowyer, C.J. Scarlett, Microwave-assisted extraction of Eucalyptus robusta leaf for the optimal yield of total phenolic compounds. Ind. Crops Prod. 69, 290–299 (2015).  https://doi.org/10.1016/j.indcrop.2015.02.044 CrossRefGoogle Scholar
  31. 31.
    M.Z. Anđelković, A.S. Milenković-Andjelković, B. Radovanović, A. Radovanović, Optimization of ultrasound-assisted extraction of phenols from seeds of grape pomace. Acta Chim. Slov. 61(4), 858–865 (2014)Google Scholar
  32. 32.
    E. Eroglu, I. Tontul, A. Topuz, Optimization of aqueous extraction and spray drying conditions for efficient processing of hibiscus blended rosehip tea powder. J. Food Process. Pres. 42(6), e13643 (2018).  https://doi.org/10.1111/jfpp.13643 CrossRefGoogle Scholar
  33. 33.
    B. Widom, Collision theory of chemical reaction rates. J. Adv. Chem. Phys. 5, 353–386 (1962)Google Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Food Engineering, Faculty of Engineering and ArchitectureNecmettin Erbakan UniversityKonyaTurkey

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