Electrochemical determination of β-lactoglobulin in whey proteins

  • Ozge Surucu
  • Serdar AbaciEmail author
Original Paper


Determination of β-lactoglobulin in milk products is very important because β-lactoglobulin is the main ingredient in whey-based protein powders. However, β-lactoglobulin is a dangerous food allergen. On such an occasion, the determination of β-lactoglobulin is coming into prominence and electrochemistry is a good alternative for this purpose because of its simple, economic and rapid response. In this work, a graphene oxide modified pencil graphite electrode is developed to determine β-lactoglobulin based on the current signal of known concentration of hydrogen peroxide. Cyclic voltammetry technique is performed to obtain the electrochemical behavior of β-lactoglobulin. Linear range, limit of detection and limit of quantification were calculated according to the calibration curve of various amounts of β-lactoglobulin. Ultraviolet–visible spectroscopy technique is also used to investigate the absorption behavior of β-lactoglobulin in various biological macromolecules including whey proteins. The proposed graphene oxide modified pencil graphite surface is successfully applied to determine β-lactoglobulin in real milk sample, so a new methodology based on a newly developed electrochemical technique is described as a promising alternative in diary products.


β-Lactoglobulin Hydrogen peroxide Whey protein Graphene oxide Pencil graphite electrode 



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Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of ScienceHacettepe UniversityBeytepe, AnkaraTurkey
  2. 2.Department of Chemistry, Faculty of ScienceEge UniversityBornova, IzmirTurkey
  3. 3.Advanced Technologies Application and Research CenterHacettepe UniversityBeytepe, AnkaraTurkey

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