Journal of Food Measurement and Characterization

, Volume 13, Issue 4, pp 2862–2870 | Cite as

Physicochemical, functional and structural characterization of Mexican Oxalis tuberosa starch modified by cross-linking

  • Lucila Concepción Núñez-Bretón
  • Liliana Catalina Cruz-Rodríguez
  • María Luisa Tzompole-Colohua
  • Jaime Jiménez-Guzmán
  • María de Jesús Perea-Flores
  • Walfred Rosas-Flores
  • Francisco Erik González-JiménezEmail author
Original Paper


Chemical modification of the native starch of Mexican Oxalis tuberosa was studied by cross-linking it with different concentrations of epichlorohydrin (0.5–2%). The results showed an extraction yield of 65.1 ± 0.20% (dry basis) for native starch, which can be considered as a potential unconventional source for starch extraction. The amylose contents of native and modified starch decreased from 24.66 to 13.57%, respectively. X-ray diffraction analysis showed an increase in the crystalline part in modified starch. The chemical modification changed the functional properties of starch. The clarity of the paste determined by spectrophotometer at 650 nm showed an inversely proportional relationship with the epichlorohydrin concentration. The lipid absorption index showed an increase up to 210% (using 2% epichlorohydrin) compared to that of native starch. The structure was analyzed by SEM and showed granules before and after the modification an ellipsoid morphology while the polarized light microscopy analysis showed birefringence patterns. The average diameter of the granules evaluated using a particle size analyzer (CILAS) ranged between 31.46 μm and 35.32 μm for modified starch and was 30.36 μm for the native starch, both higher than that of the corn-starch granules (16.10 μm). This makes the Mexican O. tuberosa an option for starch extraction and for application in the food industry.


Amylose content Particle size Epichlorohydrin Birefringence 



The obtaining of results of this work counted with contributions of PFCE (Program for Strengthening Educational Quality-SEP) 2017 resources. Financial resources of a public nature. Its use is prohibited for personal or partisan promotion purposes.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest regarding the publication of this paper.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Lucila Concepción Núñez-Bretón
    • 1
  • Liliana Catalina Cruz-Rodríguez
    • 2
  • María Luisa Tzompole-Colohua
    • 2
  • Jaime Jiménez-Guzmán
    • 2
  • María de Jesús Perea-Flores
    • 3
  • Walfred Rosas-Flores
    • 4
  • Francisco Erik González-Jiménez
    • 2
    Email author
  1. 1.Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados “CINVESTAV-IPN”Instituto Politécnico NacionalCiudad de MéxicoMéxico
  2. 2.Facultad de Ciencias QuímicasUniversidad VeracruzanaOrizabaMéxico
  3. 3.Centro de Nanociencias y Micro y Nanotecnologías del Instituto Politécnico NacionalCiudad de MéxicoMéxico
  4. 4.Departamento de Ingeniería Química y BioquímicaTecnológico Nacional de México/I.T. Durango.DurangoMéxico

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