Characterization of quality and pharmacological assessment of Pimpinella anisum L. (Anise) seeds cultivars

  • Apu Ghosh
  • Md. Moshfekus Saleh‐e‐InEmail author
  • Mirza Md. Abukawsar
  • Md. Aminul Ahsan
  • Md. Matiur Rahim
  • Md. Nurul Huda Bhuiyan
  • Sudhangshu Kumar Roy
  • Shamsun Naher
Original Paper


Pimpinella anisum L. seeds of the two cultivars were assessed for their nutritional quality and safety assessment through proximate, chemical and pharmacological studies. The proximate composition along with rich mineral elements as well as minor level of toxic elements indicated the good quality of seeds. The GC–MS analyses of hydro distilled volatile oils of two cultivars contained 16 constituents. The main constituents in BSRC (Bangladesh Spice Research Centre) seeds were trans-anethole (83.67%), fenchone (5.29%) and 1,2-diisopropenylcyclobutane (6.31%) whereas, trans-anethole (69.94%), fenchone (11.184%) and d-limonene (13.007%) were the main constituents in local market seeds. The IC50 values of DPPH antioxidant activity showed at 48.71 and 52.48 mg/mL, in BSRC and local market seeds cultivars essential oils respectively. The antimicrobial activity of the essential oils showed moderate activity in both cultivars against Gram-positive, Gram-negative bacteria and fungi. Both oils had some similarity in physiochemical properties and antimicrobial activity. The brine shrimp cytotoxic activity of the essential oils exhibited weaker activity than the standard drug at 3.06 and 2.86 μg/mL (IC50) in BSRC and local market seed cultivars respectively. The present investigation demonstrated good nutritional quality with rich bioactive phytoconstituents as well as low level of toxic elements of anise seeds. The research findings can open up a new possibility for exportation of indigenously cultivated spices as well as recommendations for food and pharmaceutical industries.


Pimpinella anisum L. Anise seed Essential oil p-anethole Elemental composition Antimicrobial Antioxidant Cytotoxicity 



Atomic absorption spectrometry


Association of official analytical chemist


Ascorbic acid




Dimethyl sufoxide


Dry weight basis


Gas chromatography and mass spectroscopy


Inductively coupled plasma mass spectrometry


Nutrient Agar


Potato Dextrose Agar


Retention Index (Kovalts index)


Retention time



Authors are thankful to the Director IFST and the Director BCSIR Laboratories, Dhaka, Bangladesh for giving the permission to conduct the research work.

Compliance with ethical standards

Conflict of interest

The authors indicate no potential conflicts of interest.

Human and animal participants

This article does not contain any studies with human or animal subjects.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Apu Ghosh
    • 1
  • Md. Moshfekus Saleh‐e‐In
    • 2
    Email author
  • Mirza Md. Abukawsar
    • 1
  • Md. Aminul Ahsan
    • 2
  • Md. Matiur Rahim
    • 3
  • Md. Nurul Huda Bhuiyan
    • 3
  • Sudhangshu Kumar Roy
    • 4
  • Shamsun Naher
    • 1
  1. 1.Department of ChemistryJagannath UniversityDhakaBangladesh
  2. 2.Institute of National Analytical Research and Services (INARS), BCSIR LaboratoriesBangladesh Council of Scientific and Industrial ResearchDhakaBangladesh
  3. 3.Food Toxicology Research Section, IFSTBangladesh Council of Scientific and Industrial ResearchDhakaBangladesh
  4. 4.IFRDBangladesh Council of Scientific and Industrial Research (BCSIR)DhakaBangladesh

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