Antioxidant activity and protective effects of Saccharomyces cerevisiae peptide fractions against H2O2-induced oxidative stress in Caco-2 cells
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Oxidative stress is one of the most causes of some severe diseases and the spoilage of foods. Protein hydrolysates containing antioxidant peptides are a suitable candidate to replace synthetic antioxidant. In the present study, the in vitro and cellular antioxidant properties of Saccharomyces cerevisiae protein hydrolysate and the peptide fractions have been reported. The peptide protective role was evaluated in H2O2-stimulated Caco-2 cells to consider cell viability, cellular lipid and protein oxidation, and the level of cellular antioxidant enzymes such as Catalase (CAT) and Glutathione-S-transferase (GST). The peptide fractions showed a significant ferric reducing antioxidant power (67.10–93.52 µm FeSO4/mg protein), and < 3 kDa peptide fraction with 59.5% inhibitory effect on the 7th day, exhibited the most inhibitory activity toward linoleic acid peroxidation. Ultrafiltered fractions ( < 3 kDa and 3–5 kDa) significantly (P ≤ 0.05) decreased the levels of malondialdehyde (MDA) and protein carbonyl and the production of CAT and GST enzymes as protective responses of cells under oxidative stress by H2O2. This study confirms the antioxidant activity of yeast protein hydrolysate and peptide fractions and their potential to reduce cellular oxidative stress and thus validates their potential use as a valuable ingredient of functional foods.
KeywordsPeptides Yeast extract Oxidative stress Cellular antioxidants
This work has been supported by Iran National Science Foundation (INSF) (Grant Number: 95824648). We gratefully acknowledge the Persian Type Culture Collection (PTCC) of the Iranian Research Organization for Science and Technology (IROST) for supplying the strain of yeast used in this study. The authors wish to thank Sanaz Jafari for her help on this project.
- 3.A.M. Giuffrè, C. Zappia, M. Capocasale, Physicochemical stability of blood orange juice during frozen storage. Int. J. Food Prop. 20, 1930–1943 (2017)Google Scholar
- 23.A.L. Rao, G.G. Sankar, Caco-2 cells: an overview. Asian J. Pharm. Res. Health Care 1, 260–275 (2009)Google Scholar
- 25.T. Osawa, M. Namiki, A novel yype of antioxidant isolated from leaf wax of leaves. Agric. Biol. Chem. 45, 735–739 (1981)Google Scholar
- 28.G. Colombo, M. Clerici, M.E. Garavaglia, D. Giustarini, R. Rossi, A. Milzani, I. Dalle-Donne, A step-by-step protocol for assaying protein carbonylation in biological samples. J. Chromatogr. B 15, 178–190 (2015)Google Scholar
- 30.W.H. Habig, M.J. Pabst, W.B. Jakoby, Glutathione S-transferases the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130–7139 (1974)Google Scholar
- 31.H.M.M. Hassan, Antioxidant and immunostimulating activities of yeast (Saccharomyces cerevisiae) autolysates. World Appl. Sci. J. 15, 1110–1119 (2011)Google Scholar
- 48.A. Dua, N. Kaur, P. Gupta, A. Mittall, S.K. Gupta, Oxidative stress induced cell damage and antioxidant enzyme response in human lymphocytes. Int. J. Pharm. Biol. Arch. 8, 33–39 (2017)Google Scholar
- 50.N. Polidoros Alexios, G. Scandalios John, Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S-transferase gene expression in maize (Zea mays L). Physiologia. Plantarum 106, 112–120 (2002).Google Scholar
- 52.E. Birben, U.M. Sahiner, C. Sackesen, S. Erzurum, O. Kalayci, Oxidative stress and antioxidant defense. WAO J. 5, 9–19 (2012)Google Scholar