Advertisement

Journal of Food Measurement and Characterization

, Volume 13, Issue 3, pp 2461–2469 | Cite as

Determination of biologically active phenolic compounds in propolis by LC–MS/MS according to seasons and altitudes

  • Ali SorucuEmail author
  • Hasan Hüseyin Oruç
Original Paper
  • 24 Downloads

Abstract

The present study aimed to determine the effect of season and altitude to amount and prevalence of phenolic compounds in propolis. For the aim, 174 propolis samples were collected from 58 stationary apiaries from altitudes of 0–200 m, 200–500 m and above 500 m in spring, summer, and autumn distributed in the Marmara region of Turkey. Ethanol-extracted propolis samples were analyzed by LC–MS/MS to determine nine flavonoids and seven phenolic acids. Significant differences were found in average levels of galangin, naringenin, pinocembrin, quercetin, luteolin, caffeic acid, trans-cinnamic acid, p-coumaric acid, m-coumaric acid, and CAPE across the seasons and altitudes (P < 0.05). However, no differences were found levels of rutin, apigenin, kaempferol, ferulic acid and gallic acid (P < 0.05). Most of the significant differences were found between spring and autumn (10 differences). The prevalence of flavonoids was generally lower than phenolic acids. CAPE and pinocembrin had majority of the significant differences in phenolic compounds according to season and altitude. The prevalence of pinocembrin 100%, m-coumaric acid 99.4%, CAPE 99.4%, naringenin 99.4%, caffeic acid 98.9%, p-coumaric acid 98.3%, quercetin 96% and luteolin 95.4% were determined that dominant phenolic compound of Marmara Region of Turkey’s propolis. Furthermore, Salix spp. 75.8%, Quercus spp. 74.4%, Populus spp. 72.4%, and Pinus spp. 53.5% were mainly determined source of propolis around the apiaries. These results show that different sampling of seasons and altitudes effect chemical composition of propolis even from the same area. The study indicated that seasons and altitudes are important factors for standardization of propolis.

Keywords

Propolis Standardization Phenolic compounds Season Altitude LC–MS/MS 

Notes

Acknowledgements

The authors would like to thank all the Marmara region’s Beekeeper Associations and the beekeepers who helped us collect propolis samples from their hives and also thanks helping us for analyses Hasan Hüseyin Ünal and Erol Kabil from Istanbul Pendik Veterinary Control Institute.

Funding

The project was supported by TÜBİTAK (1120017).

Supplementary material

11694_2019_166_MOESM1_ESM.docx (1004 kb)
Supplementary file1 Experimental details relating to this article are available in Supplementary Material, alongside Supplementary Table 1-3 and Supplementary Fig. 1-6 (DOCX 1003 kb)

References

  1. 1.
    E. Ghisalberti, Bee world 60, 59 (1979)CrossRefGoogle Scholar
  2. 2.
    V.S. Bankova, S.L. de Castro, M.C. Marcucci, Apidologie 31, 3 (2000)CrossRefGoogle Scholar
  3. 3.
    M.I. Isla, J.F. Paredes-Guzman, M.I. Nieva-Moreno, H. Koo, Y.K. Park, J. Agric. Food Chem. 53, 1166 (2005)CrossRefGoogle Scholar
  4. 4.
    S. Kumazawa, J. Nakamura, M. Murase, M. Miyagawa, M.R. Ahn, S. Fukumoto, Naturwissenschaften 95, 781 (2008)CrossRefGoogle Scholar
  5. 5.
    A.H. Banskota, Y. Tezuka, S. Kadota, Phytother. Res. 15, 561 (2001)CrossRefGoogle Scholar
  6. 6.
    A. Borges, C. Ferreira, M.J. Saavedra, M. Simoes, Microb. Drug Resist. 19, 256 (2013)CrossRefGoogle Scholar
  7. 7.
    T.W. Chung, S.K. Moon, Y.C. Chang, J.H. Ko, Y.C. Lee, G. Cho, S.H. Kim, J.G. Kim, C.H. Kim, Faseb. J. 18, 1670 (2004)CrossRefGoogle Scholar
  8. 8.
    T.P.T. Cushnie, A.J. Lamb, Int. J. Antimicrob. Ag. 26, 343 (2005)CrossRefGoogle Scholar
  9. 9.
    K.H. Janbaz, S.A. Saeed, A.H. Gilani, Fitoterapia 73, 557 (2002)CrossRefGoogle Scholar
  10. 10.
    R. Liu, M. Gao, Z.H. Yang, G.H. Du, Brain Res. 1216, 104 (2008)CrossRefGoogle Scholar
  11. 11.
    K.S.D. Mota, G.E.N. Dias, M.E.F. Pinto, A. Luiz-Ferreira, A.R.M. Souza-Brito, C.A. Hiruma-Lima, J.M. Barbosa, L.M. Batista, Molecules 14, 979 (2009)CrossRefGoogle Scholar
  12. 12.
    E.N. Quiroga, D.A. Sampietro, J.R. Soberon, M.A. Sgariglia, M.A. Vattuone, J. Appl. Microbiol. 101, 103 (2006)CrossRefGoogle Scholar
  13. 13.
    V. Bankova, J. Ethnopharmacol. 100, 114 (2005)CrossRefGoogle Scholar
  14. 14.
    H.H. Oruc, A. Sorucu, H.H. Unal, L. Aydin, Vet. J. Ankara Univ. 64, 13 (2017)Google Scholar
  15. 15.
    M. Popova, S. Silici, O. Kaftanoglu, V. Bankova, Phytomedicine 12, 221 (2005)CrossRefGoogle Scholar
  16. 16.
    L. Stan, L.A. Mărghitaş, D. Dezmirean, Sci. Papers Anim. Sci. Biotechnol. 44, 137 (2011)Google Scholar
  17. 17.
    B. Andelkovic, L. Vujisic, I. Vuckovic, V. Tesevic, V. Vajs, D. Godevac, J. Pharmaceut. Biomed. 135, 217 (2017)CrossRefGoogle Scholar
  18. 18.
    B. Bueno-Silva, A. Marsola, M. Ikegaki, S.M. Alencar, P.L. Rosalen, Nat. Prod. Res. 31, 1318 (2017)CrossRefGoogle Scholar
  19. 19.
    M.A. Calegari, A. Prasniewski, C. da Silva, R.Y. Sado, F.M.C. Maia, L.M.S. Tonial, T.L.C. Oldoni, An Acad. Bras. Cienc. 89, 45 (2017)CrossRefGoogle Scholar
  20. 20.
    M.L. Castro, J.A. Cury, P.L. Rosalen, S.M. Alencar, M. Ikegaki, S. Duarte, H. Koo, Quim. Nova. 30, 1512 (2007)CrossRefGoogle Scholar
  21. 21.
    D. Goðevac, B. Anðelković, V. Vajs, V. Tešević, Planta Med. 82, 215 (2016)Google Scholar
  22. 22.
    M.I. Isla, I.C. Zampini, R.M. Ordonez, S. Cuello, B.C. Juarez, J.E. Sayago, M.I.N. Moreno, M.R. Alberto, N.R. Vera, E. Bedascarrasbure, A. Alvarez, F. Cioccini, L.M. Maldonado, J. Med. Food. 12, 1334 (2009)CrossRefGoogle Scholar
  23. 23.
    C.A. Nunes, M.C. Guerreiro, J. Sci. Food Agric. 92, 433 (2012)CrossRefGoogle Scholar
  24. 24.
    J.M. Sforcin, A. Fernandes, C.A.M. Lopes, V. Bankova, S.R.C. Funari, J. Ethnopharmacol. 73, 243 (2000)CrossRefGoogle Scholar
  25. 25.
    E.A. Souza, R. Zaluski, N. Veiga, R.O. Orsi, Braz. J. Biol. 76, 396 (2016)CrossRefGoogle Scholar
  26. 26.
    E.W. Teixeira, D. Message, G. Negri, A. Salatino, P.C. Stringheta, Evid Based Complement Altern. Med. 7, 307 (2010)CrossRefGoogle Scholar
  27. 27.
    D. Valencia, E. Alday, R. Robles-Zepeda, A. Garibay-Escobar, J.C. Galvez-Ruiz, M. Salas-Reyes, M. Jimenez-Estrada, E. Velazquez-Contreras, J. Hernandez, C. Velazquez, Food Chem. 131, 645 (2012)CrossRefGoogle Scholar
  28. 28.
    M.P. Popova, V.S. Bankova, S. Bogdanov, I. Tsvetkova, C. Naydenski, G.L. Marcazzan, A.G. Sabatini, Apidologie 38, 306 (2007)CrossRefGoogle Scholar
  29. 29.
    S. Erdogan, B. Ates, G. Durmaz, I. Yilmaz, T. Seckin, Food Chem. Toxicol. 49, 1592 (2011)CrossRefGoogle Scholar
  30. 30.
    B. Trusheva, M. Popova, V. Bankova, S. Simova, M.C. Marcucci, P.L. Miorin, F.D. Pasin, I. Tsvetkova, Evid Based Complement. Altern. Med. 3, 249 (2006)CrossRefGoogle Scholar
  31. 31.
    F. Pellati, G. Orlandini, D. Pinetti, S. Benvenuti, J. Pharm. Biomed. Anal. 55, 934 (2011)CrossRefGoogle Scholar
  32. 32.
    M.C. Marcucci, F. Ferreres, C. Garcia-Viguera, V.S. Bankova, S.L. de Castro, A.P. Dantas, P.H.M. Valente, N. Paulino, J. Ethnopharmacol. 74, 1052 (2001)CrossRefGoogle Scholar
  33. 33.
    R. Aliyazicioglu, H. Sahin, O. Erturk, E. Ulusoy, S. Kolayli, Int. J. Food. Prop. 16, 277 (2013)CrossRefGoogle Scholar
  34. 34.
    L.C. Nunes, A.B. Galindo, S.R. Lustosa, M.T. Brasileiro, A.A. Do Egito, R.M. Freitas, K.P. Randau, P.J. Rolim Neto, Adv. Stud. Biol. 5, 119 (2013)Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Veterinary Pharmacology and Toxicology, Faculty of Milas Veterinary MedicineMuğla Sıtkı Koçman UniversityMilasTurkey
  2. 2.Department of Pharmacology and Toxicology, Faculty of Veterinary MedicineUludag UniversityNiluferTurkey

Personalised recommendations