Journal of Food Measurement and Characterization

, Volume 13, Issue 3, pp 2203–2210 | Cite as

Quantification and monitoring the heat-induced formation of trans fatty acids in edible oils by Raman Spectroscopy

  • Weichao Gong
  • Ruyi Shi
  • Min Chen
  • Jianyuan Qin
  • Xiangjiang LiuEmail author
Original Paper


Trans fatty acids (TFAs), a type of unsaturated fatty acids, has been linked to increased risks of coronary heart disease and other health problems. Thus, efficient and reliable detection methods for TFAs are of great importance for both industrial applications and research purposes. In this study, a rapid and pretreatment-free quantification approach was developed for TFAs in 13 edible oils (including 2 soybean oils, 2 peanut oils, 2 rapeseed oils, blend oil, oleum camelliae, sunflower oil, rice oil, sesame oil, olive oil and corn oil) with Raman spectroscopy and chemometric methods. The commercial oils contained 0–0.397% (w/w) of trielaidin and each of the oils were further added with 0.1–5% of trielaidin, resulting in a total of 130 samples. Results show that the concentration of TFAs in various oils can be well-predicted based on the Raman spectral features in the region of 1640–1680 cm−1 (characteristic to C=C stretching modes), yielding a residual predictive deviation (RPD) of 4.639. This approach exhibits high sensitivity in quantification, and TFAs added in different kinds of edible oils combined with their initial levels can be successfully quantified with good descriptive statistics (Rc = 0.9657, Rcv = 0.9598, Rp = 0.9634, RMSEC = 0.351, RMSECV = 0.391 and RMSEP = 0.302). Furthermore, in order to understand the heat-induced formation of TFAs during food preparation, the TFA concentrations of the oil heated under different temperatures was investigated based on the above predication model. The results indicate that the concentration of TFAs increased sharply when the oils were heated at 169–250 °C for 10 min.


Trans fatty acid Raman spectroscopy Vegetable oil Oxidative products Quantification Heat-induced 



The authors gratefully acknowledge the financial support of National key R&D plan of China (2017YFC1601700) and Natural Sciences Fund of Zhejiang Province (No. LY17C130005). We all appreciate the experimental equipment support provided by Key Laboratory of on Site Processing Equipment for Agricultural Products, Ministry of Agriculture, P. R. China.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human or animal subjects.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Weichao Gong
    • 1
  • Ruyi Shi
    • 1
  • Min Chen
    • 1
  • Jianyuan Qin
    • 1
  • Xiangjiang Liu
    • 1
    Email author
  1. 1.College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhouChina

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