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Umami and bitterness profile of enzymatic protein hydrolysates from cultured Takifugu obscurus by-products

  • Yunfeng Yang
  • Wenli Wang
  • Yuan LiuEmail author
Original Paper
  • 35 Downloads

Abstract

Takifugu obscurus is popular in Asian markets because of its delicious taste and high nutrition. The fish heads and bones which account for a significant proportion of whole fish, are rich in protein. The objective of this study was to investigate umami and bitterness profile of protein hydrolysates from cultured Takifugu obscurus by-products. In order to obtain hydrolysates with higher the degree of hydrolysis (DH) and better sensory characteristic, its hydrolysis condition was optimized using a response surface methodology (RSM). The model equations for the effects of temperature, pH and enzyme/substrate ratio (w/w, %, smashed head and bone as substrate) on DH, umami and bitterness were proposed. Protamex as the suitable enzyme was used to improve the taste profile of hydrolyzed product. The optimized model was significant and produced an experimental value (DH 48.62 ± 0.67, umami 5.25 ± 0.42 and bitterness 2.34 ± 0.39) in good agreement with the predicted value (49.21%, 5.50 and 2.04). The hydrolysate exhibited higher free amino acids (1733.19 mg/100 g) than the untreated sample (267.49 mg/100 g). The results indicate that protein hydrolysates from Takifugu obscurus by-products may potentially serve as a good source of desirable peptide and amino acids and can be used as the precursor of seafood flavoring agent.

Keywords

Takifugu obscurus by-products Protein hydrolysate Umami profile Sensory evaluation 

Notes

Acknowledgements

This work was supported by The National Key R&D Program of China (2016YFD0400803) and National Natural Science Foundation of China (Grant No. 31622042).

Compliance with ethical standards

Competing interests

The authors declared no competing financial interest.

Supplementary material

11694_2019_118_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 KB)

References

  1. 1.
    X.Q. Yu, L.J. Zhang, X.D. Miao, Y.Y. Li, Y. Liu, Food Chem. 221, 599–605 (2017)CrossRefGoogle Scholar
  2. 2.
    G. Su, C. Cui, L. Zheng, B. Yang, J. Ren, M. Zhao, Food Chem. 135, 479–485 (2012)CrossRefGoogle Scholar
  3. 3.
    Q. Shen, R. Guo, Z.Y. Dai, Y.P. Zhang, Food Chem. 60, 5192–5198 (2012)CrossRefGoogle Scholar
  4. 4.
    P.J. García-Moreno, I. Batista, C. Pires, N.M. Bandarra, F.J. Espejo-Carpio, A. Guadix, E.M. Guadix, Food Res. Int. 65, 469–476 (2014)CrossRefGoogle Scholar
  5. 5.
    X. Tan, L. Qi, F.F. Fan, Z.X. Guo, Z.Y. Wang, W. Song, M. Du, Food Chem. 264, 350–357 (2018)CrossRefGoogle Scholar
  6. 6.
    S. Egerton, S. Culloty, J. Whooley, C. Stanton, R.P. Ross, Food Chem. 245, 698–706 (2018)CrossRefGoogle Scholar
  7. 7.
    Y.P. Huang, G. Ruan, Z.J. Qin, H.Y. Li, Y.J. Zheng, Food Chem. 223, 89–95 (2017)CrossRefGoogle Scholar
  8. 8.
    Z.R. Li, B. Wang, C.F. Chi, Y.D. Gong, H.Y. Luo, G.F. Ding, Food Res. Int. 51, 283–293 (2013)CrossRefGoogle Scholar
  9. 9.
    S. Choonpicharn, S. Jaturasitha, N. Rakariyatham, N. Suree, H. Niamsup, J. Food Sci. Technol. 52, 3134–3139 (2015)CrossRefGoogle Scholar
  10. 10.
    M. Chenna, K. Messaoudi, N. Drouiche, H. Lounici, J. Ind. Eng. Chem. 33, 307–315 (2016)CrossRefGoogle Scholar
  11. 11.
    L.N. Murthy, G.G. Phadke, P. Unnikrishnan, J. Annamalai, C.G. Joshy, A.A. Zynudheen, C.N. Ravishankar, Waste Biomass Valorization. 9, 1735–1746 (2018)CrossRefGoogle Scholar
  12. 12.
    G.S. Hema, C.G. Joshy, K. Shyni, N. Chatterjee, G. Ninan, S. Mathew, J. Food Sci. Technol. 54, 488–496 (2017)CrossRefGoogle Scholar
  13. 13.
    H. Fan, M.J. Dumont, B.K. Simpson, J. Food Sci. Technol. 54, 4000–4008 (2017)CrossRefGoogle Scholar
  14. 14.
    F. Guerard, L. Guimas, A. Binet, J. Mol. Catal. B 19, 489–498 (2002)CrossRefGoogle Scholar
  15. 15.
    N. Laohakunjit, O. Selamassakul, O. Kerdchoechuen, Food Chem 158, 162–170 (2014)CrossRefGoogle Scholar
  16. 16.
    H.Y. Jung, H.S. Kwak, M.J. Kim, Y. Kim, K.O. Kim, S.S. Kim, J. Sens. Stud. 32, 1–11 (2017)CrossRefGoogle Scholar
  17. 17.
    R. da Silva, V.P.R. Minim, A.A. Simiqueli, L.E.D. Moraes, A.I. Gomide, L.A. Minim, Food Qual. Prefer. 24, 190–200 (2012)CrossRefGoogle Scholar
  18. 18.
    Q. Xia, L.P. Wang, C.C. Xu, J. Mei, Y.F. Li, Food Chem. 214, 533–542 (2017)CrossRefGoogle Scholar
  19. 19.
    N.P. Tao, L.Y. Wang, X. Gong, Y. Liu, J. Food Compos. Anal. 28, 40–45 (2012)CrossRefGoogle Scholar
  20. 20.
    Q. Sun, B. Zhang, Q.J. Yan, Z.Q. Jiang, Food Chem. 213, 708–713 (2016)CrossRefGoogle Scholar
  21. 21.
    I.W.Y. Cheung, E.C.Y. Li-Chan, Food Chem. 122, 1003–1012 (2010)CrossRefGoogle Scholar
  22. 22.
    J.A. Vazquez, M. Blanco, A.E. Massa, I. Rodriguez Amado, R.I. Perez-Martin, Mar. Drugs. 15, 1–15 (2017)Google Scholar
  23. 23.
    F. Yu, C. Zong, S. Jin, J. Zheng, N. Chen, J. Huang, Y. Chen, F.F. Huang, Z. Yang, Y.P. Tang, G.F. Ding, Mar. Drugs. 16, 1–12 (2018)CrossRefGoogle Scholar
  24. 24.
    Y. Guo, D. Pan, M. Tanokura, Food Chem. 114, 328–333 (2009)CrossRefGoogle Scholar
  25. 25.
    S.F. See, L.L. Hoo, A.S. Babji, Int. Food Res. J. 4, 1359–1365 (2011)Google Scholar
  26. 26.
    J. Roslan, S.M.M. Kamal, K.F.M. Yunos, N. Abdullah, Sains Malays. 43, 1715–1723 (2014)Google Scholar
  27. 27.
    J. Wasswa, J. Tang, X.H. Gu, J. Food Biochem. 32, 460–473 (2008)CrossRefGoogle Scholar
  28. 28.
    Y. Komata, The Taste and Constituents of Foods (Nippon Shokuhin Kogyo GakkaishI, 1969), p. 26Google Scholar
  29. 29.
    W. He, R. Yang, W. Zhao, Food Chem. 277, 655–663 (2019)CrossRefGoogle Scholar
  30. 30.
    M.R. Rhyu, M.J. Kim, H.J. Son, Y. Kim, T. Misaka, Chem. Senses 40, 660–660 (2015)Google Scholar
  31. 31.
    M. Tamura, T. Nakatsuka, M. Tada, Y. Kawasaki, E. Kikuchi, H. Okai, Agric. Biol. Chem. 53, 319–325 (1989)Google Scholar
  32. 32.
    K. Maehashi, M. Matsuzaki, Y. Yamamoto, S. Udaka, Biosci. Biotechnol. Biochem. 63, 555–559 (1999)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Food Science and TechnologyShanghai Ocean UniversityShanghaiChina
  2. 2.Department of Food Science and Engineering, School of Agriculture & BiologyShanghai Jiao Tong UniversityShanghaiChina

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