Advertisement

Journal of Food Measurement and Characterization

, Volume 13, Issue 3, pp 1797–1804 | Cite as

A fluorescence nanobiosensor for detection of Campylobacter jejuni DNA in milk based on Au/Ag bimetallic nanoclusters

  • Zahra Dehghani
  • Morteza HosseiniEmail author
  • Javad Mohammadnejad
Original Paper
  • 50 Downloads

Abstract

We designed a rapid method for DNA sensing for detection of campylobacter jejuni based on a selective DNA probe. A sequence containing 12 cytosine nucleotides was applied as a scaffold for making Au/Ag nanocluster. This sequence along with a 30 nucleotides selective probe was used for campylobacter jejuni DNA detection. The Au/Ag nanocluster showed a turn-off fluorescence response in presence of target DNA and probe/target hybridization process. Our result showed that with gradual increase in target concentration, intensity of fluorescence sequentially decreased. Increase in target concentration from 10 to 300 pM showed linear decrease in Au/Ag nanocluster fluorescence emission. Limit of detection of this method was 4.4 pM and capability of this method in detection of selective target from non-selective DNA was considerable. This nanobiosensor was used for evaluation of bacterial target DNA spiked in milk sample and results indicated applicability of this structure for bacterial detection in medical, environmental and food samples.

Keywords

Campylobacter jejuni Fluorescence Bimetallic Au/Ag nanocluster 

Notes

Acknowledgements

The authors thank the research Council of University of Tehran (Grant 28645/01/02) and Tehran University of Medical Sciences for financial support of this work.

Funding

Financial support was provided by the Faculty of new sciences and technologies, Tehran University, Tehran (Iran).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11694_2019_98_MOESM1_ESM.docx (165 kb)
Supplementary material 1 (DOCX 165 KB)

References

  1. 1.
    M.C. Peterson, Western J. Med. 161, 148 (1994)Google Scholar
  2. 2.
    Campylobacter, Campylobacter jejuni infection and the association with Guillain-Barré syndrome, 2nd edn. (ASM Press, Washington, DC, 2000), pp. 155–175Google Scholar
  3. 3.
    M.B. Skirrow, M.J. Blaser, I. Nachamkin, M.J. Blaser, L.S. Tompkins, Clinical and epidemiologic considerations (American Society for Microbiology(Washington, D.C., 1992)Google Scholar
  4. 4.
    M.C. Samuel, D.J. Vugia, S. Shallow, R. Marcus, S. Segler, T. McGivern, H. Kassenborg, K. Reilly, M. Kennedy, F. Angulo, R.V. Tauxe, Clin. Infect. Diseases 38, S165 (2004)CrossRefGoogle Scholar
  5. 5.
    J. Neimann, J. Engberg, K. Mølbak, H.C. Wegener, Epidemiol. Infect. 130, 353 (2003)CrossRefGoogle Scholar
  6. 6.
    M.H. Josefsen, A.K. Bhunia, E.O. Engvall, M.S. Fachmann, J. Hoorfar, J. Microbiol. Methods 112, 118 (2015)CrossRefGoogle Scholar
  7. 7.
    F. Ceylan, M.G. Akpınar, A.M. Cherciov, B. Özkan, M. Gül, Int. J. Agric. Life. Sci 2(2), 47 (2018)Google Scholar
  8. 8.
    X. Yang, J. Kirsch, A. Simonian, J. Microbiol. Methods 95, 48 (2013)CrossRefGoogle Scholar
  9. 9.
    T.J. Gnanaprakasa, O.A. Oyarzabal, E.V. Olsen, V.A. Pedrosa, A.L. Simonian, Sens. Actuators B 156, 304 (2011)CrossRefGoogle Scholar
  10. 10.
    J.G. Bruno, T. Phillips, M.P. Carrillo, R. Crowell, J. Fluorescence 19, 427 (2009)CrossRefGoogle Scholar
  11. 11.
    N.A. Masdor, Z. Altintas, I.E. Tothill, Biosens. Bioelectron. 78, 328 (2016)CrossRefGoogle Scholar
  12. 12.
    J. Huang, G. Yang, W. Meng, L. Wu, A. Zhu, X.A. Jiao, Biosens. Bioelectron. 25, 1204 (2010)CrossRefGoogle Scholar
  13. 13.
    T.G. Drummond, M.G. Hill, J.K. Barton, Nat. Biotechnol. 21, 1192 (2003)CrossRefGoogle Scholar
  14. 14.
    D. Wei, O.A. Oyarzabal, T.S. Huang, S. Balasubramanian, S. Sista, A.L. Simonian, J. Microbiol. Methods 69, 78 (2007)CrossRefGoogle Scholar
  15. 15.
    A.D. Taylor, J. Ladd, Q. Yu, S. Chen, J. Homola, S. Jiang, Biosens. Bioelectron. 22, 752 (2006)CrossRefGoogle Scholar
  16. 16.
    N.A. Masdor, Z. Altintas, I.E. Tothill, Chemosensors 5, 16 (2017)CrossRefGoogle Scholar
  17. 17.
    F.E. Ahmad, J.E. Wiley, D.A. Weidner, C. Bonnerup, H. Mota, Cancer Genomics-Proteomics 7, 303 (2010)Google Scholar
  18. 18.
    M.E. Yakovleva, A.P. Moran, G.R. Safina, T. Wadström, B. Danielsson, Anal. Chim. Acta 694, 1 (2011)CrossRefGoogle Scholar
  19. 19.
    M. Manzano, F. Cecchini, M. Fontanot, L. Iacumin, G. Comi, P. Melpignano, Biosens. Bioelectron. 66, 271 (2015)CrossRefGoogle Scholar
  20. 20.
    G.K. Darbha, E. Lee, Y.R. Anderson, P. Fowler, K. Mitchell, P.C. Ray, IEEE Sens. J. 8, 693 (2008)CrossRefGoogle Scholar
  21. 21.
    X. Qu, Y. Li, L. Li, Y. Wang, J. Liang, J. Nanomater. 2015, 4 (2015)Google Scholar
  22. 22.
    A. Ahmed, J.V. Rushworth, N.A. Hirst, P.A. Millner, Clinical Microbiol. Rev. 27, 631 (2014)CrossRefGoogle Scholar
  23. 23.
    Y. Tao, M. Li, J. Ren, X. Qu, Chem. Soc. Rev. 44, 8636 (2015)CrossRefGoogle Scholar
  24. 24.
    Y.S. Borghei, M. Hosseini, M.R. Ganjali, Microchim. Acta 184, 2671 (2017)CrossRefGoogle Scholar
  25. 25.
    Y.S. Borghei, M. Hosseini, M. Khoobi, M.R. Ganjali, J. Fluorescence 27, 529 (2017)CrossRefGoogle Scholar
  26. 26.
    M. Hosseini, S. Mohammadi, Y.S. Borghei, M.R. Ganjali, J. Fluorescence 27, 1443 (2017)CrossRefGoogle Scholar
  27. 27.
    Y.S. Borghei, M. Hosseini, M.R. Ganjali, S. Hosseinkhani, Sens. Actuators B 248, 133 (2017)CrossRefGoogle Scholar
  28. 28.
    T. Zhang, H. Xu, S. Xu, B. Dong, Z. Wu, X. Zhang, L. Zhang, H. Song, RSC Adv. 6, 51609 (2016)CrossRefGoogle Scholar
  29. 29.
    C.M. Ritchie, K.R. Johnsen, J.R. Kiser, Y. Antoku, R.M. Dickson, J.T. Petty, J. Phys. Chem. C 111, 175 (2007)CrossRefGoogle Scholar
  30. 30.
    E. Shokri, M. Hosseini, F. Faridbod, M. Rahaie, J. Fluorescence 26, 51795 (2017)Google Scholar
  31. 31.
    H.A. Kermani, M. Hosseini, M. Dadmehr, M.R. Ganjali, Anal. Bioanal. Chem. 408, 4311 (2016)CrossRefGoogle Scholar
  32. 32.
    E. Shokri, M. Hosseini, F. Faridbod, M. Rahaie, RSC Adv. 6, 99437 (2016)CrossRefGoogle Scholar
  33. 33.
    M. Dadmehr, M. Hosseini, S. Hosseinkhani, M.R. Ganjali, R. Sheikhnejad, Biosens. Bioelectron. 73, 108 (2015)CrossRefGoogle Scholar
  34. 34.
    M. Hosseini, A. Akbari, M.R. Ganjali, M. Dadmehr, A.H. Rezayan, J. Fluorescence 25, 925 (2015)CrossRefGoogle Scholar
  35. 35.
    M. Hosseini, E. Ahmadi, Y.S. Borghei, M.R. Ganjali, Methods Appl. Fluoresce 5, 015005 (2017)CrossRefGoogle Scholar
  36. 36.
    J.M. Obliosca, C. Liu, R.A. Batson, M.C. Babin, J.H. Werner, H.C. Yeh, Biosensors 3, 185–200 (2013)CrossRefGoogle Scholar
  37. 37.
    Z.X. Wang, C.L. Zheng, S.N. Ding, RSC Adv. 4, 9825 (2014)CrossRefGoogle Scholar
  38. 38.
    Y. Dou, X. Yang, Anal. Chim. Acta 784, 53 (2013)CrossRefGoogle Scholar
  39. 39.
    C. Zheng, A.X. Zheng, B. Liu, X.L. Zhang, Y. He, J. Li, H.H. Yang, G. Chen, Chem. Commun. 50, 13103 (2014)CrossRefGoogle Scholar
  40. 40.
    S.Y. New, S.T. Lee, X.D. Su, Nanoscale 8, 17729 (2016)CrossRefGoogle Scholar
  41. 41.
    H. Huang, H. Li, J.J. Feng, A.J. Wang, Sens. Actuators B 223, 550 (2016)CrossRefGoogle Scholar
  42. 42.
    O.A. Oyarzabal, H. Fernández, (Springer International Publishing, New York 2016) pp. 19–35Google Scholar
  43. 43.
    S.W. Yang, T. Vosch, Anal. Chem. 83, 6935 (2011)CrossRefGoogle Scholar
  44. 44.
    E. Shokri, M. Hosseini, F. Faridbod, M. Rahaie, J. Fluorescence 26, 1795 (2016)CrossRefGoogle Scholar
  45. 45.
    G.S. Banting, C. Braithwaite Scott, J. Kim, B. Jeon, N. Ashbolt, N. Ruecker, L. Tymensen, J. Charest, K. Pintar, S. Checkley, Appl. Environ. Microbiol. 82, 4743 (2016)CrossRefGoogle Scholar
  46. 46.
    A.C. Thomas, Chem. Commun. 48, 6845 (2012)CrossRefGoogle Scholar
  47. 47.
    J. Yamagishi, Y. Sato, N. Shinozaki, B. Ye, A. Tsuboi, M. Nagasaki, R. Yamashita, PLoS ONE 11, e0154389 (2016)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zahra Dehghani
    • 1
  • Morteza Hosseini
    • 1
    • 2
    Email author
  • Javad Mohammadnejad
    • 1
  1. 1.Department of Life Science Engineering, Faculty of New Sciences & TechnologiesUniversity of TehranTehranIran
  2. 2.Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of PharmacyTehran University of Medical SciencesTehranIran

Personalised recommendations