Journal of Food Measurement and Characterization

, Volume 13, Issue 3, pp 1797–1804 | Cite as

A fluorescence nanobiosensor for detection of Campylobacter jejuni DNA in milk based on Au/Ag bimetallic nanoclusters

  • Zahra Dehghani
  • Morteza HosseiniEmail author
  • Javad Mohammadnejad
Original Paper


We designed a rapid method for DNA sensing for detection of campylobacter jejuni based on a selective DNA probe. A sequence containing 12 cytosine nucleotides was applied as a scaffold for making Au/Ag nanocluster. This sequence along with a 30 nucleotides selective probe was used for campylobacter jejuni DNA detection. The Au/Ag nanocluster showed a turn-off fluorescence response in presence of target DNA and probe/target hybridization process. Our result showed that with gradual increase in target concentration, intensity of fluorescence sequentially decreased. Increase in target concentration from 10 to 300 pM showed linear decrease in Au/Ag nanocluster fluorescence emission. Limit of detection of this method was 4.4 pM and capability of this method in detection of selective target from non-selective DNA was considerable. This nanobiosensor was used for evaluation of bacterial target DNA spiked in milk sample and results indicated applicability of this structure for bacterial detection in medical, environmental and food samples.


Campylobacter jejuni Fluorescence Bimetallic Au/Ag nanocluster 



The authors thank the research Council of University of Tehran (Grant 28645/01/02) and Tehran University of Medical Sciences for financial support of this work.


Financial support was provided by the Faculty of new sciences and technologies, Tehran University, Tehran (Iran).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11694_2019_98_MOESM1_ESM.docx (165 kb)
Supplementary material 1 (DOCX 165 KB)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zahra Dehghani
    • 1
  • Morteza Hosseini
    • 1
    • 2
    Email author
  • Javad Mohammadnejad
    • 1
  1. 1.Department of Life Science Engineering, Faculty of New Sciences & TechnologiesUniversity of TehranTehranIran
  2. 2.Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of PharmacyTehran University of Medical SciencesTehranIran

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