New selective media for isolation and enumeration of Lactobacillus rhamnosus and Streptococcus thermophilus

  • Chenchen Zhang
  • Liting Yang
  • Zixuan Ding
  • Boxing Yin
  • Dawei Chen
  • Chengran Guan
  • Ruixia GuEmail author
Original Paper


To improve the quality and function of fermented products, Lactobacillus rhamnosus and Streptococcus thermophilus are often used together. However, it was found that MRS agar medium was not efficient in distinguishing between L. rhamnosus LV108 and S. thermophilus grx02. Several modified MRS agar media were investigated in this study. MRS agar supplemented with sodium acetate could not inhibit the growth of S. thermophilus grx02. Though S. thermophilus grx02 did not grow on low pH MRS (pH 5.0), L. rhamnosus grew slowly on this medium, and its CFU count was lower than that on MRS. MRS without glucose (Gly-MRS) didn’t support the growth of S. thermophilus, but L. rhamnosus could produce well-defined colonies as it did on MRS, which were not influenced by the growth phases or culture media for the tested strains. In addition, when cocultured with L. rhamnosus LV108, the viable count of S. thermophilus grx02 could be enumerated by controlling the culture time on LM17. By restricting the carbon source and culture time, mixtures of L. rhamnosus LV108 and S. thermophilus grx02 could be enumerated rapidly and easily in 48 h (minimum 36 h), which could benefit the development, production and storage of probiotic dairy products containing these two species.


Selective plate count Lactobacillus rhamnosus Streptococcus thermophilus Gly-MRS LM17 



This work was supported by Natural Science Foundation of Jiangsu Province (CN) (BK20180910, BK20170496) and National Natural Science Foundation of China (No. 31571855, 31701627).

Compliance with ethical standards

Conflict of interest

No conflict of interest declared.


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Authors and Affiliations

  1. 1.College of Food Science and EngineeringYangzhou UniversityYangzhouPeople’s Republic of China
  2. 2.Jiangsu Key Laboratory of Dairy Biotechnology and Safety ControlYangzhouPeople’s Republic of China
  3. 3.Jiangsu Dairy Biotechnology Engineering Research CenterYangzhouPeople’s Republic of China

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